Real-time RT-PCR Detection of HCN4 and ADAM8 genes in ventilator-associated pneumonia patients Hospitalized in intensive care unit

authors:

avatar Hamidreza Jamaati 1 , * , avatar Naghmeh Bahrami , avatar Masoum Abniki 2 , avatar Payam Tabarsi 3 , avatar Behrooz Farzanegan 4 , avatar Atosa Doroudinia 5 , avatar Abdolreza Mohamadnia ORCID 6

Chronic Respiratory Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Biotechnology, Faculty of Sciences, Islamic Azad University, Tehran, Iran
Clinical Tuberculosis and Epidemiology Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
Tracheal Diseases Research Center, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
Pathologist, National Research Institute of Tuberculosis and Lung Diseases (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran
PhD of Molecular Medicine, Virology Research Center, National Research Institute of Tuberculosis & Lung Disease (NRITLD), Shahid Beheshti University of Medical Sciences, Tehran, Iran, Islamic Republic of
Journal of Cellular & Molecular Anesthesia: Vol.1, issue 4; e149519
published online: August 26, 2016
article type: Original Articles
DOI: https://doi.org/10.22037/jcma.v1i4.13372

how to cite: Jamaati H, Bahrami N, Abniki M, Tabarsi P, Farzanegan B, et al. Real-time RT-PCR Detection of HCN4 and ADAM8 genes in ventilator-associated pneumonia patients Hospitalized in intensive care unit. J Cell Mol Anesth. 2016;1(4):e149519. https://doi.org/10.22037/jcma.v1i4.13372.

Abstract

IntroductionVentilator-associated pneumonia (VAP) ?is One of the most serious and? prevalence of? complication? among patients in the intensive care unit nosocomial infections, which are often recognized after detecting symptoms. To date, there has been no proper clinical and diagnostic marker for early detection of this disease. In this study, two HCN4 and ADAM8 genes in patients with VAP were assessed to be used as a biomarker to recognize and distinguish the disease. ??MethodologyThis study was done in Masih Daneshvari Hospital affiliated to Shahid Beheshti University of Medical Sciences,Tehran,Iran since 2015-2016 and was case /control study . current study was administered by using peripheral blood samples, including 30 patients with VAP and 30 Healthy person. First, the peripheral blood samples were taken and then RNA was extracted and then synthesis CDNA. Finally, the evaluation of genes was performed by Realtime PCR method. ??FindingsIn peripheral blood samples, patients individuals , 10 out of 30 cases had positive HCN4 markers. Among the healthy individuals, 6 out of 30 cases had positive HCN4 ?biomarkers.?Moreover, in ADAM8 marker patients individuals, 13 out of 30 cases had positive ADAM8 marker and 8 out of 30 healthy individuals had positive ADAM8 biomarkers.ConclusionGenes HCN4 and ADAM8 ?assessment with Real Time-PCR? in this study can be used as promising markers in early detection of VAP disease. More extensive studies on larger sample sizes may yield higher sensitivity for these molecular markers.

References

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