The effect of aqueous extract of Malva neglecta on expression of inflammatory biomarkers involved in pain in synoviocytes and THP-1 cells as a model of monocyte/macrophage and human cartilage cells in osteoarthritis

Reza Taherian; Mahdi Taherian; Hossein Maghsoudi; Samaneh Haj-alahyari

doi:10.22037/jcma.v2i4.18291

Journal of Cellular & Molecular Anesthesia

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https://doi.org/10.22037/jcma.v2i4.18291

The effect of aqueous extract of Malva neglecta on expression of inflammatory biomarkers involved in pain in synoviocytes and THP-1 cells as a model of monocyte/macrophage and human cartilage cells in osteoarthritis

Author(s):

Reza Taherian^1,*,

Mahdi Taherian²,

Hossein Maghsoudi³,

Samaneh Haj-alahyari⁴

1Student Research Committee, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2Food and Drug Research Institute, Iran Food and Drug Administration, Ministry of Health and Medical Education, Tehran, Iran

3Biotechnology Research Center, Payamenoor University, Tehran, Iran

4Payamenoor University, Tehran, Iran

Journal of Cellular & Molecular Anesthesia:Vol. 2, issue 4; e149555

Published online:Dec 11, 2017

Article type:Original Articles

How to Cite:Reza TaherianMahdi TaherianHossein MaghsoudiSamaneh Haj-alahyariThe effect of aqueous extract of Malva neglecta on expression of inflammatory biomarkers involved in pain in synoviocytes and THP-1 cells as a model of monocyte/macrophage and human cartilage cells in osteoarthritis.J Cell Mol Anesth.2(4):e149555.https://doi.org/10.22037/jcma.v2i4.18291.

Abstract

Background: Osteoarthritis (OA) is now considered as an active complex of biomechanical, biochemical and cellular processes, not a mere degenerative disorder. Considering the complications of common treatments of OA, including non-steroidal anti-inflammatory drug (NSAIDs) and corticosteroids, establishment of new treatments is crucial. This study aimed to explore the effect of Malva Neglecta extract on the main inflammatory biomarkers in OA.Materials and Methods: Aqueous extract of Malva Neglecta, ibuprofen and betamethasone were prepared to investigate their effects on inflammatory biomarkers. Synoviocytes were obtained from the healthy radiocarpal joint cartilage of an 8-month-old Holstein cow. Human monocyte/macrophage (THP-1) cells were also obtained to investigate the effect of Malva neglecta extract on inflammatory agents. Lipopolysaccharide (LPS) was used to induce production of inflammatory cytokines in both cells. Real-time PCR was used to investigate the effect of Malva Neglecta extract on expression profile of TNF-?, IL-1?, COX-2, IL-18 and iNOS. Production of NO and PGE2 was also investigated in THP-1 cells.Results: Malva Neglecta extract reduced TNF-?, IL-1?, iNOS, IL-18 and COX-2 expression in synoviocytes. Expression of all of these factors was also reduced by the extract in THP-1 cells. Moreover, production of PGE2 and NO in the LPS-induced THP-1 cells was reduced by Malva neglecta extract. Ibuprofen and betamethasone were more effective in reducing inflammatory agents than the extract.Conclusion: According to Malva?s ability to reduce the pro-inflammatory cytokines in the synoviocytes and THP-1 cells, its potential role as a supplement method to common NSAIDs and corticosteroids was confirmed.

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Author(s):

Reza Taherian:[PubMed][Scholar]
Mahdi Taherian:[PubMed][Scholar]
Hossein Maghsoudi:[PubMed][Scholar]
Samaneh Haj-alahyari:[PubMed][Scholar]