Effect of bupivacaine and combination with dexmedetomidine and dexamethasone on mice neural apoptosis

authors:

avatar Mohammadreza Moshari 1 , * , avatar Ali Dabbagh ORCID 2 , avatar Mastaneh Dahi Taleghani ORCID 2 , avatar Maryam Vosoughian 1 , avatar Behnam Hosseini 2 , avatar Fereshteh Baghizadeh 2 , avatar Seyed Mohammad Seyed -Alshohadaei 2

Anesthesiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Anesthesiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Journal of Cellular & Molecular Anesthesia: Vol.4, issue 3; e149589
published online: January 28, 2020
article type: Original Articles
DOI: https://doi.org/10.22037/jcma.v4i3.28282

how to cite: Moshari M, Dabbagh A, Dahi Taleghani M, Vosoughian M, Hosseini B, et al. Effect of bupivacaine and combination with dexmedetomidine and dexamethasone on mice neural apoptosis. J Cell Mol Anesth. 2019;4(3):e149589. https://doi.org/10.22037/jcma.v4i3.28282.

Abstract

Background: Numerous studies have shown the neurotoxicity of anesthetic substances in different age groups. This toxicity is often associated with damage or apoptosis of nerve cells that can lead to various diseases, including Alzheimer's, behavioral changes and transient and even persistent cognitive changes. In this study, it was attempted to evaluate the cytotoxic conditions following the use of three common anesthetic drugs (bupivacaine, dexmedetomidine and dexamethasone) by providing a suitable substrate.Methods and Materials: Mice (Mus musculus) with the same weight (22 to 30 gr) were used for assessment of neurotoxicity in Bupivacaine, Dexmedetomidine and Dexamethasone. Unilateral femoral nerve injections were done; animals were randomly divided into four groups: control, bupivacaine alone, "bupivacaine + dexmedetomidine" and "bupivacaine + dexamethasone". After 24 hours, the mice were sacrificed and the femoral nerve removed. Hematoxylin-eosin tissue staining was used to evaluate changes in the effects of the drugs, and nerve samples were extracted to assess the expression of TLR4 and caspase3. Protein expression level was checked between different groups using Western blot technique.Results: The bupivacaine + dexamethasone group showed better outcomes in terms of cytotoxicity than bupivacaine + dexmedetomidine (p=0.568); also, bupivacaine + dexamethasone reduced neurotoxicity risk (P=0.431).Conclusion: Bupivacaine+dexamethasone leeds to better outcomes in terms of neurotoxicity compared with bupivacaine+dexmedetomidine.

References

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    References are in the PDF file of the article.