The effects of Ropivacaine and its Combination with Dexmedetomidine and Dexamethasone on Neural Apoptosis

Mohammadreza Moshari; Behnam Hosseini; Ali Dabbagh; Faramarz Mosaffa; Shideh Dabir; seyed mohammad seyed alshohadaei; Fereshteh Baghizadeh

doi:10.22037/jcma.v5i3.30086

Journal of Cellular & Molecular Anesthesia

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https://doi.org/10.22037/jcma.v5i3.30086

The effects of Ropivacaine and its Combination with Dexmedetomidine and Dexamethasone on Neural Apoptosis

Author(s):

Mohammadreza Moshari¹,

Behnam Hosseini^1,*,

Ali Dabbagh

²,

Faramarz Mosaffa¹,

Shideh Dabir

¹,

seyed mohammad seyed alshohadaei¹,

Fereshteh Baghizadeh¹

1Anesthesiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

2Anesthesiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Journal of Cellular & Molecular Anesthesia:Vol. 5, issue 3; e149619

Published online:May 25, 2020

Article type:Original Articles

How to Cite:Mohammadreza MoshariBehnam HosseiniAli DabbaghFaramarz MosaffaShideh Dabirseyed mohammad seyed alshohadaeiFereshteh Baghizadehet al.The effects of Ropivacaine and its Combination with Dexmedetomidine and Dexamethasone on Neural Apoptosis.J Cell Mol Anesth.5(3):e149619.https://doi.org/10.22037/jcma.v5i3.30086.

Abstract

Background: Neurotoxicity effects of anesthetics in different age groups is a major concern regarding neural injuries. It is reasonable to recognize the neurotoxicity risks of anesthetic drugs and their combinations. Materials and Methods: A total of 16 mice (Mus musculus) weighing 22 to 30 gr were randomly divided into four drug groups (control, Ropivacaine, Ropivacaine + Dexmedetomidine, Ropivacaine + Dexamethasone). 24 hours after unilateral injection of drugs into the femoral nerve of mice, the mice were killed and their femoral nerve was removed. Hematoxylin-eosin tissue staining was used to evaluate changes in the effects of the drugs, and nerve samples were extracted to measure TLR4 and caspase 3 expressions. After Western blotting, the protein expression level was checked between different groups. Results: Ropivacaine in combination with dexamethasone caused less damage to the rat nerve cells. The combination of ropivacaine with dexamethasone (p=0.53 and p=0.46) compared to the combination of ropivacaine with dexmedetomidine relatively had better results in terms of cytotoxicity. Conclusion: A combination of ropivacaine with dexamethasone reduces neurotoxicity risk.

Keywords

Glyceraldehyde 3-phosphate dehydrogenase

Cysteine-aspartic acid protease

References

1.
References are in the PDF file of the article.

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Author(s):

Mohammadreza Moshari:[PubMed][Scholar]
Behnam Hosseini:[PubMed][Scholar]
Ali Dabbagh:[PubMed][Scholar]
Faramarz Mosaffa:[PubMed][Scholar]
Shideh Dabir:[PubMed][Scholar]
seyed mohammad seyed alshohadaei:[PubMed][Scholar]
Fereshteh Baghizadeh:[PubMed][Scholar]