https://doi.org/10.22037/jcma.v5i3.30086

The effects of Ropivacaine and its Combination with Dexmedetomidine and Dexamethasone on Neural Apoptosis

authors:

avatar Mohammadreza Moshari 1 , avatar Behnam Hosseini 1 , * , avatar Ali Dabbagh ORCID 2 , avatar Faramarz Mosaffa 1 , avatar Shideh Dabir ORCID 1 , avatar seyed mohammad seyed alshohadaei 1 , avatar Fereshteh Baghizadeh 1

Anesthesiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Anesthesiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Journal of Cellular & Molecular Anesthesia: Vol.5, issue 3; e149619
published online: May 25, 2020
article type: Original Articles

How To Cite Moshari M, Hosseini B, Dabbagh A, Mosaffa F, Dabir S, et al. The effects of Ropivacaine and its Combination with Dexmedetomidine and Dexamethasone on Neural Apoptosis. J Cell Mol Anesth. 2020;5(3):e149619. https://doi.org/10.22037/jcma.v5i3.30086.

Abstract

Background: Neurotoxicity effects of anesthetics in different age groups is a major concern regarding neural injuries. It is reasonable to recognize the neurotoxicity risks of anesthetic drugs and their combinations. Materials and Methods: A total of 16 mice (Mus musculus) weighing 22 to 30 gr were randomly divided into four drug groups (control, Ropivacaine, Ropivacaine + Dexmedetomidine, Ropivacaine + Dexamethasone). 24 hours after unilateral injection of drugs into the femoral nerve of mice, the mice were killed and their femoral nerve was removed. Hematoxylin-eosin tissue staining was used to evaluate changes in the effects of the drugs, and nerve samples were extracted to measure TLR4 and caspase 3 expressions. After Western blotting, the protein expression level was checked between different groups. Results: Ropivacaine in combination with dexamethasone caused less damage to the rat nerve cells. The combination of ropivacaine with dexamethasone (p=0.53 and p=0.46) compared to the combination of ropivacaine with dexmedetomidine relatively had better results in terms of cytotoxicity. Conclusion: A combination of ropivacaine with dexamethasone reduces neurotoxicity risk.

References

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    References are in the PDF file of the article.