The effects of Ropivacaine and its Combination with Dexmedetomidine and Dexamethasone on Neural Apoptosis

authors:

avatar Mohammadreza Moshari 1 , avatar Behnam Hosseini 1 , * , avatar Ali Dabbagh ORCID 2 , avatar Faramarz Mosaffa 1 , avatar Shideh Dabir ORCID 1 , avatar seyed mohammad seyed alshohadaei 1 , avatar Fereshteh Baghizadeh 1

Anesthesiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Anesthesiology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Journal of Cellular & Molecular Anesthesia: Vol.5, issue 3; e149619
published online: May 25, 2020
article type: Original Articles
DOI: https://doi.org/10.22037/jcma.v5i3.30086

how to cite: Moshari M, Hosseini B, Dabbagh A, Mosaffa F, Dabir S, et al. The effects of Ropivacaine and its Combination with Dexmedetomidine and Dexamethasone on Neural Apoptosis. J Cell Mol Anesth. 2020;5(3):e149619. https://doi.org/10.22037/jcma.v5i3.30086.

Abstract

Background: Neurotoxicity effects of anesthetics in different age groups is a major concern regarding neural injuries. It is reasonable to recognize the neurotoxicity risks of anesthetic drugs and their combinations. Materials and Methods: A total of 16 mice (Mus musculus) weighing 22 to 30 gr were randomly divided into four drug groups (control, Ropivacaine, Ropivacaine + Dexmedetomidine, Ropivacaine + Dexamethasone). 24 hours after unilateral injection of drugs into the femoral nerve of mice, the mice were killed and their femoral nerve was removed. Hematoxylin-eosin tissue staining was used to evaluate changes in the effects of the drugs, and nerve samples were extracted to measure TLR4 and caspase 3 expressions. After Western blotting, the protein expression level was checked between different groups. Results: Ropivacaine in combination with dexamethasone caused less damage to the rat nerve cells. The combination of ropivacaine with dexamethasone (p=0.53 and p=0.46) compared to the combination of ropivacaine with dexmedetomidine relatively had better results in terms of cytotoxicity. Conclusion: A combination of ropivacaine with dexamethasone reduces neurotoxicity risk.

References

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    References are in the PDF file of the article.