Neuroapoptosis Signaling Pathways in Hippocampus Following Ovariectomy and Its Inhibition by Systemic Estradiol Replacement Therapy

1Laser Application in Medical Sciences Research Center & Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Cardiovascular Disease Research Institute, Tehran Heart Center, Tehran, Iran

2Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3Hearing Disorders Research Center, Loghman Hakim Hospital & Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4Department of Anatomical Sciences, School of Medicine, Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran

5Department of Microbiology, Karaj Branch, Karaj, Islamic Azad University, Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran

6Assistant professor, Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Tehran

7Neuroscience Research Center & Department of Anatomy, School of Medicine, Iran University of Medical Sciences, Tehran, Iran

Journal of Cellular & Molecular Anesthesia:Vol. 8, issue 2; e149685

Published online:Oct 10, 2022

Article type:Original Articles

How to Cite:Mana JameieElahe TorabiMaryam GolmohammadiSaeed Vafaei-NezhadMona FarhadiHojjat-allah AbbaszadehSeyed Behnamedin Jameieet al.Neuroapoptosis Signaling Pathways in Hippocampus Following Ovariectomy and Its Inhibition by Systemic Estradiol Replacement Therapy.J Cell Mol Anesth.8(2):e149685.https://doi.org/10.22037/jcma.v8i2.39264.

Abstract

In recent decades, there is a growing concern about neurodegenerative diseases (NDs) resulting from the increasing pattern of population age and life expectancy. Although there is no medical treatment for NDs, certain studies have suggested the neuroprotective role of estradiol (ES) in the brain. Hence hormone replacement therapy would be a promising therapeutic alternative for NDs. The present study was performed to investigate the beneficial effect of ES in the neurogenesis and neuro-apoptosis signaling pathways in the hippocampus following ovariectomy (OVX). Thirty Wistar rats were randomly assigned to 5 groups: 1) control 2) OVX, 3) OVX and ES, 4) Surgical sham, and 5) vehicle shams for ES. Eight weeks after surgery, OVX and ES group received an intramuscular injection of ES (20?g/rat) for 30 days, then hippocampi were obtained from all rats for molecular and histology studies. Nissl staining of the hippocampus for neuronal cell counting and western blot for detecting the expression of caspase-3 and cytochrome-c were performed. Based on our findings using western blot, overexpression of apoptotic proteins in the hippocampal tissue was observed in the OVX group. Furthermore, Nissl staining revealed a significant hippocampal neuronal cell death in the OVX group. In a nutshell, ES could result in the increase of neuron cells and the prevention of apoptosis in the hippocampus.

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Author(s):

Mana Jameie:[PubMed][Scholar]
Elahe Torabi:[PubMed][Scholar]
Maryam Golmohammadi:[PubMed][Scholar]
Saeed Vafaei-Nezhad:[PubMed][Scholar]
Mona Farhadi:[PubMed][Scholar]
Hojjat-allah Abbaszadeh:[PubMed][Scholar]
Seyed Behnamedin Jameie:[PubMed][Scholar]