Insulin Can Improve the Normal Function of the Brain by Preventing the Loss of the Neurons

authors:

avatar Ali Asghar Peyvandi 1 , avatar Hojjat Allah Abbaszadeh ORCID 2 , avatar Mohammad Golshan Tafti 3 , avatar Mohammad-Amin Abdollahifar ORCID 4 , avatar Hojjat-allah Abbaszadeh ORCID 5

Hearing Disorders Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Pediatrics, Islamic Azad University of Medical Sciences, Yazd, Iran
Hearing Disorders Research Center, Loghman Hakim Hospital and Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Assistant professor, Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Tehran
Warning: No corresponding author defined!
Journal of Cellular & Molecular Anesthesia: Vol.7, issue 4; e149750
published online: June 07, 2022
article type: Original Articles
DOI: https://doi.org/10.22037/jcma.v7i4.38324

how to cite: Peyvandi A A , Abbaszadeh H A, Golshan Tafti M, Abdollahifar M, Abbaszadeh H. Insulin Can Improve the Normal Function of the Brain by Preventing the Loss of the Neurons. J Cell Mol Anesth. 2022;7(4):e149750. https://doi.org/10.22037/jcma.v7i4.38324.

Abstract

Background: Insulin promotes the expression of genes related to brain function, thus preventing the neurodegeneration process. The present study was designed to find the neuroprotective effect of insulin by reducing neuron loss in the brain. Materials and Methods: In this study, 20 adult male NMRI mice were divided into two groups: control and insulin. The control group was intact, and the insulin group received 100 ?L of insulin at a 72-hour interval by intraperitoneal (I.P.) injection for 30 days. At the end of the study, the brain was removed. The volume of the brain and the total number of neurons and glia were estimated by stereological techniques, and also the gene expression of NSR, PI3K, AKT, IGF-1, and FOXO-1 was measured using real-time PCR. Results: The results showed that the total number of neurons decreased in the control group compared to the experimental group. Furthermore, the expression of NSR, PI3K, AKT, IGF-1, and FOXO-1 genes was lower in the control group than in the insulin group. Conclusion: The results showed that treating mice with insulin prevented reducing the number of neurons and gene expression related to normal brain function. So, insulin could have neuroprotective effects against neuron loss. Insulin may be beneficial as a new approach to avoiding neuron loss in regenerative medicine.

References

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    References are in the PDF file of the article.