Abstract
Background: To determine the effects of Apis dorsata honey on the development of morphine tolerance and oxidative stress in rats. Materials and Methods: A total of 40 male Sprague Dawley rats were injected (subcutaneous) with 10 mg/kg of morphine following oral administration of A. dorsata honey (0.5, 1.5, and 2.5g/kg). On day 15, the rats were euthanized, and the thalamus, spinal cord, and hippocampus were homogenized to assess iNOS and MDA using ELISA kits.Results: The honey of A. dorsata significantly prevented morphine tolerance to analgesia in the hotplate test on Day 14 (p<0.05). The biochemical assessment showed that A. dorsata honey significantly reduced MDA formation in the brain regions compared to the morphine control group at dose 2.5g/kg. Elevation of iNOS caused by chronic morphine intake was reduced in A. dorsata honey co-treatment. Conclusion: This study suggests the therapeutic role of A. dorsata honey in preventing morphine tolerance via inhibition of oxidative stress.
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Copyright
© 2021, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.