MDMA-Induced BV2 Microglial Cell Activation in Vitro

Nor Suliana Mustafa; Nor Hidayah Abu Bakar; Ramli Musa; Nasir Mohamad; Nor Zidah Ahmad; Mohd Nazri Mohd Daud

doi:10.22037/jcma.v7i3.38360

Journal of Cellular & Molecular Anesthesia

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https://doi.org/10.22037/jcma.v7i3.38360

MDMA-Induced BV2 Microglial Cell Activation in Vitro

Author(s):

Nor Suliana Mustafa¹,

Nor Hidayah Abu Bakar ²,

Ramli Musa³,

Nasir Mohamad¹,

Nor Zidah Ahmad¹,

Mohd Nazri Mohd Daud⁴

1Faculty of Medicine, Sultan Zainal Abidin University, Medical Campus, 20400, Kuala Terengganu, Terengganu, Malaysia

2Faculty of Medicine, Sultan Zainal Abidin University, Medical Campus, Kuala Terengganu, Terengganu, Malaysia

3Kulliyyah of Medicine, International Islamic University Malaysia, Jalan Sultan Ahmad Shah, Bandar Indera Mahkota, 25200 Kuantan, Pahang, Malaysia

4Department Of Community and Family Medicine, Faculty Of Medicine And Health Science, Universiti Malaysia Sabah, Sabah, Malaysia

Journal of Cellular & Molecular Anesthesia:Vol. 7, issue 3; e150206

Published online:May 13, 2022

Article type:Original Articles

How to Cite:Nor Suliana MustafaNor Hidayah Abu Bakar Ramli MusaNasir MohamadNor Zidah AhmadMohd Nazri Mohd Daudet al.MDMA-Induced BV2 Microglial Cell Activation in Vitro.J Cell Mol Anesth.7(3):e150206.https://doi.org/10.22037/jcma.v7i3.38360.

Abstract

Introduction: 3,4-Methylenedioxymethamphetamine (MDMA) is a type of psychostimulant drug that induces neurotoxicity. Even though several psychostimulant substances activate microglia, little is known about MDMA's effects on these cells, and evidence of MDMA-induced microglial activation is equivocal. Material and Methods: This study employed a murine microglial cell line, BV2, to examine the effects of MDMA on the microglia morphological changes and the survival of microglia in vitro. MDMA was incorporated into the media at the time of plating and cell number and levels of mitochondrial dehydrogenase activity (MTT) were determined in vitro. The level of pro-inflammatory cytokine TNF-? was also determined. Result: Treatment of BV2 cells with MDMA resulted in morphological changes, reduced cell viability after 24h incubation with the inhibitory concentration (IC50) value of 243.6 ?g/mL, and increase TNF-? level in a dose-dependent manner. Conclusion: These findings proposed that MDMA could induce BV2 microglial cell activation in vitro and suggested that it has an important role in the development of MDMA use disorder.

Keywords

3

4-methylenedioxymethamphetamine

MDMA

microglial activation

neurotoxicity

References

1.
References are in the PDF file of the article.

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Author(s):

Nor Suliana Mustafa:[PubMed][Scholar]
Nor Hidayah Abu Bakar :[PubMed][Scholar]
Ramli Musa:[PubMed][Scholar]
Nasir Mohamad:[PubMed][Scholar]
Nor Zidah Ahmad:[PubMed][Scholar]
Mohd Nazri Mohd Daud:[PubMed][Scholar]