Evaluation of the Neuroprotective Effect of Chalcone on P53 and Caspase III Expression and D2-Like Dopaminergic Receptor Up-Regulation in vitro Parkinson's Model

Seyed Behnamedin Jameie; Melika Sadat Jamaei; Zahra Azimzadeh; Mona Farhadi; Navid Ahmady Roozbahany; Hojjat Allah Abbaszadeh

doi:10.5812/jcma-151424

Journal of Cellular & Molecular Anesthesia

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https://doi.org/10.22037/jcma.v8i1.38877

Evaluation of the Neuroprotective Effect of Chalcone on P53 and Caspase III Expression and D2-Like Dopaminergic Receptor Up-Regulation in vitro Parkinson's Model

Author(s):

Seyed Behnamedin Jameie^1,*,

Melika Sadat Jamaei²,

Zahra Azimzadeh^{3, 4},

Mona Farhadi⁵,

Navid Ahmady Roozbahany⁴,

Hojjat Allah Abbaszadeh

³

1Neuroscience Research Center, Iran University of Medical Sciences, Tehran, Iran

2Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3Laser Application in Medical Sciences Research Center, Shahid Beheshti University ofMedical Sciences, Tehran, Iran

4Hearing Disorders Research Center, Loghman Hakim Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

5Department of Microbiology, Islamic Azad University, Karaj Branch, Karaj, Iran

Journal of Cellular & Molecular Anesthesia:Vol. 8, issue 1; e151424

Published online:Sep 16, 2022

Article type:Research Article

How to Cite:Seyed Behnamedin JameieMelika Sadat JamaeiZahra AzimzadehMona FarhadiNavid Ahmady RoozbahanyHojjat Allah Abbaszadehet al.Evaluation of the Neuroprotective Effect of Chalcone on P53 and Caspase III Expression and D2-Like Dopaminergic Receptor Up-Regulation in vitro Parkinson's Model.J Cell Mol Anesth.8(1):e151424.https://doi.org/10.22037/jcma.v8i1.38877.

Abstract

Background: Parkinson's disease (PD) is a progressive neurodegenerative disorder of the central nervous system (CNS). Several studies indicated abnormal cell death in neurodegenerative diseases. Chalcone is a compound of natural origin with various properties such as antioxidant, anti-inflammatory, and inhibition of apoptosis. We investigated the impact of chalcone in an in-vitro model of PD.
Materials and Methods: PC12 cells were divided into four groups. Negative control, 6-hydroxy dopamine (6-OHDA) group (treatment with 75µM 6-OHDA), sham (treated with dimethyl sulfoxide), and the experimental groups with different dosages of chalcone treatment. Cell viability and reactive oxygen species (ROS) were assessed by MTT and ROS kit, respectively. The expressions of D2-like receptors, P53, and caspase III were evaluated by Western blotting.
Results: We found that 6-OHDA induced cytotoxicity and ROS production. The viability results showed that all doses of chalcone significantly increased viability after 48 hours compared to the control group (P<0.01). The western blotting results showed that caspase III and P53 expression decreased significantly in the experimental groups compared to the 6-OHDA group. However, D2-like receptor expression did not significantly differ between the experimental and the 6-OHDA group.
Conclusion: Complementary therapies, such as the use of antioxidants and the chalcone family, along with standard treatments for neurodegenerative diseases such as Parkinson's, may reduce the symptoms of the disease.

Keywords

Chalcone

Dopaminergic Receptors

Apoptosis

Parkinson’s Disease

References

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References are in the PDF file of the article.

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Author(s):

Seyed Behnamedin Jameie:[PubMed][Scholar]
Melika Sadat Jamaei:[PubMed][Scholar]
Zahra Azimzadeh:[PubMed][Scholar]
Mona Farhadi:[PubMed][Scholar]
Navid Ahmady Roozbahany:[PubMed][Scholar]
Hojjat Allah Abbaszadeh:[PubMed][Scholar]