Identification of patients with Cutaneous Leishmaniasis using microscopic observation and PCR methods

Alisha  Akia; Yazdan  Hamzavi; Naser  Nazari; Esmat  Rashiditabar

Journal of Clinical Research in Paramedical Sciences

The Official Journal of Paramedical School, KUMS

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Identification of patients with Cutaneous Leishmaniasis using microscopic observation and PCR methods

Author(s):

Alisha Akia¹,

Yazdan Hamzavi^2,*,

Naser Nazari²,

Esmat Rashiditabar³

1Department of Medical Microbiology, School of medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Department of Medical Parasitology and Mycology,School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

3Department of Medical Laboratory Sciences, School of Paramedicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Clinical Research in Paramedical Sciences:Vol. 5, issue 3; e81496

Published online:Sep 03, 2016

Article type:Research Article

Received:Feb 10, 2016

Accepted:Jul 17, 2016

How to Cite:Alisha Akia, Yazdan Hamzavi, Naser Nazari, Esmat Rashiditabar, Identification of patients with Cutaneous Leishmaniasis using microscopic observation and PCR methods.J Clin Res Paramed Sci.2016;5(3):e81496.

Abstract

Introduction: Leishmaniasis is endemic in more than 88 countries and threatens more than 350 million people. Until recently, diagnosis was based primarily on clinical symptoms and microscopic observation of parasites in stained tissue smears.PCR methods using either genomic or kinetoplast DNA (kDNA) are now frequently used as the most sensitive test for screening the suspected patients of cutaneous Leishmaniasis. In this study, we calculated the sensitivity, specificity, positive and negative predictive value of microscopic observation in diagnosis of cutaneous Leishmaniasis compared with the PCR technique as the gold standard.

Methods: Skin biopsies stored in ethanol from 45 patients with suspected cutaneous Leishmaniasis (CL) were tested in a polymerase chain reaction (PCR) assay as the gold standard. Then the sensitivity, specificity, positive and negative predictive value of conventional diagnostic method of microscopic observation of Giemsa stained smears were calculated.

Results: The sensitivity, specificity, positive and negative predictive value of conventional diagnostic method of microscopic observation was 71%, 100%, 100% and 71.9%, respectively.

Conclusion The PCR technique has the more ability in distinguishing healthy subjects from patients than microscopic observation of Giemsa stained smears.

Keywords

sensitivity

specificity

positive and negative predictive value

microscopic observation

cutaneous Leishmaniasis

PCR

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