The spread of aminoglycoside resistant genes in clinical isolates of Klebsiella pneumoniae from Kermanshah medical centers

Alisha  Akya; Roya  Chegene Lorestani; Azam  Elahi

Journal of Clinical Research in Paramedical Sciences

The Official Journal of Paramedical School, KUMS

Accepted Manuscripts Current Issue Archive In Press Search

Journal Information Boards and Committees Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Publication Malpractice Statement Reviewer and AE Registration Form Support Contact Us

Authors Guide Submit Manuscript

The spread of aminoglycoside resistant genes in clinical isolates of Klebsiella pneumoniae from Kermanshah medical centers

Author(s):

Alisha Akya¹,

Roya Chegene Lorestani²,

Azam Elahi^2,*

1Nosocomial Infection Research Center, Faculty of Medicine,Kermanshah University of Medical Sciences, Kermanshah, Iran

2Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Clinical Research in Paramedical Sciences:Vol. 5, issue 4; e81939

Published online:Feb 08, 2017

Article type:Research Article

Received:Jun 14, 2016

Accepted:Dec 09, 2016

How to Cite:Akya A, Chegene Lorestani R, Elahi A. The spread of aminoglycoside resistant genes in clinical isolates of Klebsiella pneumoniae from Kermanshah medical centers. J Clin Res Paramed Sci. 2017;5(4):e81939. doi:

Abstract

Introduction: Resistance of Klebsiella pneumoniae to aminoglycosides is a serious therapeutic problem, especially in hospitals. The aim of this study was to determine resistance to aminoglycosides and frequency of aac (3)-IIa, aac (6')- Ib, aac (3)-IV, aph (3')- Ia and ant (2")- Ia K. pneumoniae isolated from Kermanshah Medical Centers.

Methods: A total of 165 samples from different clinical samples were investigated and among them 100 isolates of K. pneumoniae by Keith API-20E was confirmed. The antibiotic sensitivity test to aminoglycosides by disk diffusion method was performed. After extraction the bacterial genome, the genes of aac (3)- Ia, aac (3)-IV, aph (3')-Ia, aac (6')- Ib and ant (2")- Ia were tested using PCR. Data are statistically analyzed.

Results: Of 100 isolates, 49 isolates (49%) were resistant to tobramycin and / or gentamycin. Frequency of aac (6')-Ib, aph (3')-Ia and ant (2")-Ia among isolates were 87.7%, 42.8% and 24.4%, respectively. But aac (3)-IV and aac (3)- IIa genes were not found in any of the isolates.

Conclusion: K.pneumoniae isolates phenotypically showed lower resistance aminoglyocosides tested in compared to the most previous studies. However, the aac (6 ') - Ib, aph (3') - Ia and ant (2 ") - Ia resistant genes had a high prevalence rate. The high prevalence of resistance genes in Kermanshah can be consequences of extensive and inappropriate prescribing of aminoglycosides and the lack of effective measures for infection control in hospitals.

Keywords

Klebsiella pneumoniae

Aminoglycosides genes

Resistance

Fulltext

The full text of this article is available on PDF.

References

1.
The References of this article is available on PDF.

Assessment of 16srRNA Methylase Genes Among Non-ESBL and ESBL-Producing Klebsiella pneumoniae Isolates

Iraj Pakzad,

Naser Samadi,

Mohammad Imanieini,

Morovvat Taherikalani,

Mohammad Rahbar,

Reza Heidari

,et al.

Pakzad I, Samadi N, Imanieini M, Taherikalani M, Rahbar M, et al. Assessment of 16srRNA Methylase Genes Among Non-ESBL and ESBL-Producing Klebsiella pneumoniae Isolates. Arch Clin Infect Dis. 2019;14(6):e84372. doi: https://doi.org/10.5812/archcid.84372

6

Aug

2016

Prevalence of Quinolone Resistance Genes in Klebsiella pneumoniae Strains Isolated from Hospitalized Patients During 2013 - 2014

Mohsen Heidary,

Hossein Goudarzi,

Ali Hashemi,

Gita Eslami,

Mehdi Goudarzi,

Alireza Salimi Chirani

,et al.

Heidary M, Goudarzi H, Hashemi A, Eslami G, Goudarzi M, et al. Prevalence of Quinolone Resistance Genes in Klebsiella pneumoniae Strains Isolated from Hospitalized Patients During 2013 - 2014. Arch Pediatr Infect Dis. 2017;5(4):e38343. doi: https://doi.org/10.5812/pedinfect.38343

8

May

2024

Antibiotic Susceptibility of Klebsiella pneumonia Isolates from Hospitalized Patients in Three Referral Medical Centers in Isfahan, Iran

Sayed Nassereddin Mostafavi Esfahani,

Soodabeh Rostami,

Abtin Astaraki

Mostafavi Esfahani SN, Rostami S, Astaraki A. Antibiotic Susceptibility of Klebsiella pneumonia Isolates from Hospitalized Patients in Three Referral Medical Centers in Isfahan, Iran. Zahedan J Res Med Sci. 2024;26(3):e140226. doi: https://doi.org/10.5812/zjrms-140226

8

Jul

2019

Investigating the Frequency of Klebsiella Infection and Drug Resistance Among Inpatients and Outpatients Referring to Amir Al-Momenin Hospital, Gerash, Iran

Aliyar Piruozi,

Hossein Forouzandeh,

Abbas Farahani,

Zahra Forouzandeh,

Iraj Ahmadi,

Rahman Abdizadeh

,et al.

Piruozi A, Forouzandeh H, Farahani A, Forouzandeh Z, Ahmadi I, et al. Investigating the Frequency of Klebsiella Infection and Drug Resistance Among Inpatients and Outpatients Referring to Amir Al-Momenin Hospital, Gerash, Iran. Gene Cell Tissue. 2019;6(3):e93161. doi: https://doi.org/10.5812/gct.93161

30

Sep

2014

Prevalence of Aminoglycoside Resistance Genes in Acinetobacter baumannii Isolates

Katayun Aliakbarzade,

Safar Farajnia,

Ashraf Karimi Nik,

Farzaneh Zarei,

Asghar Tanomand

Aliakbarzade K, Farajnia S, Karimi Nik A, Zarei F, Tanomand A. Prevalence of Aminoglycoside Resistance Genes in Acinetobacter baumannii Isolates. Jundishapur J Microbiol. 2014;7(10):e11924. doi: https://doi.org/10.5812/jjm.11924

Import into EndNote Import into BibTex

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 0
Last Update: 1 month ago
Scopus by Title: 0
Last Update: 1 month ago
Scopus by Title (Ref): 0
Last Update: 1 month ago
CrossRef: 0
Last Update: 5 days ago

Metrics

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Alisha Akya:[PubMed][Scholar]
Roya Chegene Lorestani:[PubMed][Scholar]
Azam Elahi:[PubMed][Scholar]

The Official Journal of Paramedical School, KUMS

Outlines

The spread of aminoglycoside resistant genes in clinical isolates of Klebsiella pneumoniae from Kermanshah medical centers

Abstract

Fulltext

References

Similar Articles

Assessment of 16srRNA Methylase Genes Among Non-ESBL and ESBL-Producing Klebsiella pneumoniae Isolates

Prevalence of Quinolone Resistance Genes in Klebsiella pneumoniae Strains Isolated from Hospitalized Patients During 2013 - 2014

Antibiotic Susceptibility of Klebsiella pneumonia Isolates from Hospitalized Patients in Three Referral Medical Centers in Isfahan, Iran

Investigating the Frequency of Klebsiella Infection and Drug Resistance Among Inpatients and Outpatients Referring to Amir Al-Momenin Hospital, Gerash, Iran

Prevalence of Aminoglycoside Resistance Genes in Acinetobacter baumannii Isolates

Last Update: 1 month ago

Last Update: 1 month ago

Last Update: 1 month ago

Last Update: 5 days ago