The spread of aminoglycoside resistant genes in clinical isolates of Klebsiella pneumoniae from Kermanshah medical centers

Alisha  Akya; Roya  Chegene Lorestani; Azam  Elahi

Journal of Clinical Research in Paramedical Sciences

The Official Journal of Paramedical School, KUMS

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The spread of aminoglycoside resistant genes in clinical isolates of Klebsiella pneumoniae from Kermanshah medical centers

Author(s):

Alisha Akya¹,

Roya Chegene Lorestani²,

Azam Elahi^2,*

1Nosocomial Infection Research Center, Faculty of Medicine,Kermanshah University of Medical Sciences, Kermanshah, Iran

2Department of Microbiology, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Clinical Research in Paramedical Sciences:Vol. 5, issue 4; e81939

Published online:Feb 08, 2017

Article type:Research Article

Received:Jun 14, 2016

Accepted:Dec 09, 2016

How to Cite:Akya A, Chegene Lorestani R, Elahi A. The spread of aminoglycoside resistant genes in clinical isolates of Klebsiella pneumoniae from Kermanshah medical centers.J Clin Res Paramed Sci.2017;5(4):e81939.

Abstract

Introduction: Resistance of Klebsiella pneumoniae to aminoglycosides is a serious therapeutic problem, especially in hospitals. The aim of this study was to determine resistance to aminoglycosides and frequency of aac (3)-IIa, aac (6')- Ib, aac (3)-IV, aph (3')- Ia and ant (2")- Ia K. pneumoniae isolated from Kermanshah Medical Centers.

Methods: A total of 165 samples from different clinical samples were investigated and among them 100 isolates of K. pneumoniae by Keith API-20E was confirmed. The antibiotic sensitivity test to aminoglycosides by disk diffusion method was performed. After extraction the bacterial genome, the genes of aac (3)- Ia, aac (3)-IV, aph (3')-Ia, aac (6')- Ib and ant (2")- Ia were tested using PCR. Data are statistically analyzed.

Results: Of 100 isolates, 49 isolates (49%) were resistant to tobramycin and / or gentamycin. Frequency of aac (6')-Ib, aph (3')-Ia and ant (2")-Ia among isolates were 87.7%, 42.8% and 24.4%, respectively. But aac (3)-IV and aac (3)- IIa genes were not found in any of the isolates.

Conclusion: K.pneumoniae isolates phenotypically showed lower resistance aminoglyocosides tested in compared to the most previous studies. However, the aac (6 ') - Ib, aph (3') - Ia and ant (2 ") - Ia resistant genes had a high prevalence rate. The high prevalence of resistance genes in Kermanshah can be consequences of extensive and inappropriate prescribing of aminoglycosides and the lack of effective measures for infection control in hospitals.

Keywords

Klebsiella pneumoniae

Aminoglycosides genes

Resistance

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