article information

Jundishapur Journal of Natural Pharmaceutical Products: Vol.11, issue 4; e29911
published online: October 2, 2016
article type: Brief Report
received: May 22, 2015
revised: February 20, 2016
accepted: February 27, 2016
DOI: https://doi.org/10.17795/jjnpp-29911
Crossmark

The Effect of Hydroalcoholic Extract of Artemisia dracunculus L. (Tarragon) on Candida albicans Infection in Mice

authors:

avatar Mahnaz Alasvand Zarasvand 1 , * , avatar Mahboobeh Madani 1 , avatar Mehrdad Modaresi 2

Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, IR Iran
Department of Physiology, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, IR Iran

how to cite: Alasvand Zarasvand M, Madani M, Modaresi M. The Effect of Hydroalcoholic Extract of Artemisia dracunculus L. (Tarragon) on Candida albicans Infection in Mice. Jundishapur J Nat Pharm Prod. 2016;11(4):e29911. https://doi.org/10.17795/jjnpp-29911.

Abstract

Background:

Candida albicans, an opportunistic pathogen, causes disseminated infection in immunocompromised host. Artemisia dracunculus L. (Tarragon) is a plant used as meal and remedy for a long time, and possesses some medicinal characteristics. Its pharmacological properties have been shown in a large number of in-vitro and in-vivo studies.

Objectives:

Regarding the antifungal activity, widespread secondary metabolites, and proved biological activities of tarragon, this study aimed to investigate the effect of hydroalcoholic extract of A. dracunculus L. on C. albicans infection in mice.

Methods:

The mice were divided into six groups including a normal control group, a placebo group, and three treatment groups receiving 50, 100, and 200 mg/kg doses of hydroalcoholic extract via intraperitoneal (ip) injection followed by inoculation with C. albicans. A control+ group, inoculated only with C. albicans, was also employed. After all treatments, the mice were killed by cervical decapitation and the concentration of C. albicans was measured in the liver and kidney homogenates.

Results:

The results showed a significant decrease in C. albicans concentration in the liver and kidney homogenates in the treatment groups (P < 0.05).

Conclusions:

It can be concluded that hydroalcoholic extract of A. dracunculus L. improves C. albicans infection in mice as a result of possessing antifungal effects.

1. Background

Candida albicans is an opportunistic pathogen that causes serious diseases in immunocompromised patients. It was reported that C. albicans is the fourth cause of nosocomial infections, having a mortality rate of up to 40% (1). There is much evidence that show C. albicans proteins have immunosuppression property (2). Pathogenesis of C. albicans is related to virulence factors such as kinds of adhesins, phospholipases, and aspartyl proteases enzyme (3). Also, C. albicans is considered as a dimorphic pathogen because of its ability to transition from yeast to hyphae (4). The risk factors for candidemia include lesion of mucosal surface (due to chemotherapy), surgical intervention, neutropenia, change of digestion system (due to the use of broad-spectrum antibiotics), acquired immune deficiency syndrome (AIDS), neoplastic disorders, and breaking skin. Innate immunity is the dominant protective mechanism against candidiasis (4, 5). Amphotericin B is the common drug applied to treat disseminated candidiasis in spite of its reported toxicity (6). Cell-mediated immunity is essential for the host defense against C. albicans infections. Neutrophils and monocytes can damage and kill yeast cells, hyphae, and pseudohyphae (5). Despite the development of new antifungal drugs, mortality associated with candidiasis remains high (7).

Recently, treating many diseases with medicinal plants has been increased (8). The low efficiency, high doses, and increasing side effects of synthetic medicine has led human to use natural products (9, 10). Also, plant drugs are more economic than synthetic ones (9). Artemisia dracunculus L. (tarragon) is a perennial plant that is classified in the Asteraceae family. French tarragon (also named German tarragon) and Russian tarragon are two main varieties of this plant (11). It is used as an herbal medicine because it provides health benefits. In traditional medicine, it had been used to improve the function of digestive system, flush toxins from the body, treat insomnia, and act as both anesthetic and antiepileptic agent. In the current therapeutics, it is used to alleviate the nervous system (as an antiepileptic agent) and improve the function of digestive and renal systems; it also possesses anti-inflammatory, anticancer, and antibacterial effects (11). The most important biologically active secondary metabolites in tarragon are essential oils, coumarins, flavonoids, and phenolcarbonic acids (12). Considering the therapeutic effects of tarragon, this study was conducted to investigate the effects of hydroalcoholic extract of A. dracunculus L. on C. albicans infection in mice.

2. Methods

2.1. Animals

In this study, 48 male mice (30 ± 5 g) were provided from Pasteur institute of Iran and divided into six groups. They were housed in cages at 22 ± 2°C in a cycle of 12:12 hours light / dark while they had free access to water and food. This study was ethically approved by the Ethical committee of Islamic Azad University, Falavarjan Branch.

2.2. Preparation of Tarragon Extract

The plant was identified by Isfahan center for research of agricultural science and natural resources. The leaves were separated, dried in the shade, and powdered using an electric grinder. Fifty grams of the powder was moved to a sterile Erlenmeyer and mixed with ethyl alcohol until the powder suspended. The mix was shaken for 48 hours and after that, it was filtered using filter paper. The obtained liquid was heated in an oven at 40°C until the alcohol evaporated. The residual was the pure extract of the plant. By adding a given amount of normal saline, three doses of extract (50, 100, and 200 mg/kg) were prepared and refrigerated at 4°C for injections.

2.3. Microorganism

C. albicans (ATCC 1677) was cultured on sabouraud dextrose agar (SDA) media and put in incubator at 37°C for 48 hours. The characteristics of yeast cells were scrutinize by microscopic examination, colony shape on SDA, and germ tubes forming in serum and then culturing on BBL™ CHROMagar™ Candida (a selective medium for the isolation and presumptive identification of yeast and filamentous fungi and differentiation of Candida species).

2.4. Experiment

48 mice were divided into six groups of eight members: a normal control group, a placebo group that received 0.5 mL of normal saline, three treatment groups that received 50, 100, and 200 mg/kg doses of each hydroalcoholic extract by intraperitoneal (ip) injection followed by inoculation with C. albicans (after the fifth injection) to get experimental infection, and a control+ group that was only inoculated with C. albicans. The extract injections were administered in the next 20 days (10 injections). C. albicans was inoculated intraperitoneally with 0.2 mL of 105 CFU/mL suspension of organism in a sterile saline (6). It was confirmed by quantitative culture technique and counting by a hemocytometer.

2.5 Quantification of Microorganism in Organs

After the final injection, the mice were anaesthetized and killed by cervical decapitation. The animals were autopsied aseptically immediately after the death. To measure C. albicans, the liver and kidney were removed, weighed, rinsed, and homogenized in 3 mL normal saline. Afterwards, 1 mL of the dilutions were plated on Petri dishes containing SDA and incubated for 48 hours at 37°C. The CFU/g was determined in both organs.

2.6. Statistical Evaluation

All data were expressed as mean ± SEM for the eight animals in each group. Statistical analysis was conducted using analysis of variance (ANOVA) followed by Tukey’s Post Hoc in SPSS software. A P < 0.05 was considered as significance criterion.

3. Results

Table 1 indicates C. albicans concentrations in the liver and kidney homogenates in all of the groups. It is deduced that there is a more significant decrease ( P < 0.05 ) in the concentration of C. albicans in the two treatment groups receiving 100 and 200 mg/kg doses than the control+ group.

Table 1.

C. albicans Concentration in the Liver and Kidney in the Defined Groups

GroupsLiver Mean CFU, g-1 ± SEMKidney Mean CFU, g-1 ± SEM
Normal Control 0.0 ± 0.00.0 ± 0.0
Placebo0.0 ± 0.00.0 ± 0.0
Treatment, 50 mg/kg34.42 ± 6.85b15.51 ± 2.56b
Treatment, 100 mg/kg2.71 ± 2.21a0.0 ± 0.0a
Treatment, 200 mg/kg16.08 ± 9.32b0.0 ± 0.0a
Control+36.28 ± 7.8053.31 ± 25.70
Between groups Sig.0.0060.021

bP < 0.01 compared to control+ group

aP < 0.05 compared to control+ group

4. Discussion

Plants have the ability to synthesize secondary metabolites like proteins, flavonoids, alkaloids, steroids, and phenolic substances that have medicinal properties. The medicinal plants can enhance the resistance of the body against many infections and disorders because of their immunomodulatory activities (13). Many studies have shown that A. dracunculus L. has therapeutic uses, and a number of studies confirm its beneficial medicinal properties (11). In the present research, the effect of hydroalcoholic extract of A. dracunculus L. was studied on C. albicans infection in mice. The results showed C. albicans cells decreased in the treatment groups in comparison with the control+ group. Experimental candidiasis in this study was a sub-acute systemic infection which was tolerated by the mice (14). Neutrophils and macrophages are responsible for the host defense against a C. albicans infection (7). These defensive cells express surface receptors that recognize foreign substances in blood or tissues and stimulate the phagocytosis procedure. Neutrophils and macrophages also have receptors that activate the cells to produce cytokines (groups of proteins that mediate many of responses in innate and adaptive immunity). C. albicans is killed by oxidative mechanisms, including generation of reactive oxygen and nitrogen intermediate and non-oxidative mechanisms, as well (5). Neutrophils, also called polymorphonuclear (PMN) leucocytes, are recruited by granulocyte-colony stimulating factor (G-CSF). Treatment of mice with recombinant (rG-CSF) can lead to the significant reduction of mortality during disseminated candidiasis (15). As described previously, A. dracunculus L. has secondary metabolites such as essential oils, flavonoids, etc. The extensive researches have shown that the essential oils in Artemisia spp. are effective against C. albicans and volatile fractions of Artemisia plants exhibit antifungal in vitro activity (16, 17). A. dracunculus has been reported to act as antifungal agent against Phythium ultimum, Sclerotinia sclerotiorum, Botrytis spp., Fusarium seminectum, Colletrotichum fragariae, Collectrotichum gloeosporioides, and Collectrotichum acutatum (11). It has been shown that some flavonoid compounds have antifungal and anti-Candida spp. activities (18). In addition, antimicrobial and antioxidant activity of flavonoid components have been confirmed previously (19, 20). As the innate immunity plays the dominant role in candidiasis (5), it seems that A. dracunculus L. might boost the mentioned mechanism, too. The anti-candidiasis effects of other medicinal plants were reported in some researches. It has been shown that the treatment with garlic extract can decrease C. albicans cells in the liver and kidney in the diabetic rats (14). Effects of Aloe vera gel extract on the response of macrophages to C. albicans indicated that R100 fraction is the most effective fraction of A. vera (21). In conclusion, our results suggest that A. dracunculus L. can exhibit anti-fungal activity, and improve C. albicans infection in mice due to possessing effective anti-candida metabolites. To find out the effective component(s) and anti-fungal mechanism(s) of the plant in this approach, we need to isolate and purify tarragon components. Also, investigation on the effect of other extracts (aquatic, acetonic, etc.) of A. dracunculus L. on other infections and diseases is also suggested.

Acknowledgements

References

  • 1.

    Li W, Hu X, Zhang X, Ge Y, Zhao S, Hu Y, et al. Immunisation with the glycolytic enzyme enolase confers effective protection against Candida albicans infection in mice. Vaccine. 2011;29(33):5526-33. [PubMed ID: 21645574]. https://doi.org/10.1016/j.vaccine.2011.05.030.

  • 2.

    Holakoee M, Yadegari MH, Saraf ZH, Mahdavi M, Eskandari A. Evaluation the Effect of Candida Albicans Infections (Candidiasis) on Tumor Mass Status, intra Tumor CD4/CD8 Ratio of the Mammary Tumor in Balb/C Mice. Trauma Monthly. 2007;2007(1, Spring):29-40.

  • 3.

    Calderone RA, Fonzi WA. Virulence factors of Candida albicans. Trends Microbiol. 2001;9(7):327-35. [PubMed ID: 11435107].

  • 4.

    Bineshian F, Yadegari MH, Sharifi Z, Akbari Eidgahi M, Nasr R. Identification of Candida Species Using MP65 Gene and Evaluation of the Candida albicans MP65 Gene Expression in BALB/C Mice. Jundishapur J Microbiol. 2015;8(5):18984. [PubMed ID: 26060567]. https://doi.org/10.5812/jjm.8(5)2015.18984.

  • 5.

    Shoham S, Levitz SM. The immune response to fungal infections. Br J Haematol. 2005;129(5):569-82. [PubMed ID: 15916679]. https://doi.org/10.1111/j.1365-2141.2005.05397.x.

  • 6.

    Dhuley JN. Hamycin treatment of candidiasis in normal and diabetic rats. FEMS Immunol Med Microbiol. 1999;26(2):175-80. [PubMed ID: 10536305].

  • 7.

    Stuyt RJ, Netea MG, van Krieken JH, van der Meer JW, Kullberg BJ. Recombinant interleukin-18 protects against disseminated Candida albicans infection in mice. J Infect Dis. 2004;189(8):1524-7. [PubMed ID: 15073691]. https://doi.org/10.1086/382955.

  • 8.

    Ziaee T, Razavi BM, Hosseinzadeh H. Saffron reduced toxic effects of its constituent, safranal, in acute and subacute toxicities in rats. Jundishapur J Nat Pharm Prod. 2014;9(1):3-8. [PubMed ID: 24644432].

  • 9.

    Kesmati M, Izadi L, Mard-Soltani M. Effect of Matricaria recutita Hydroalcoholic Extract on Anxiety Behavior in Mice by Hole-Board Test. Zahedan J Res Med Sci. 2014;16(3):21-4.

  • 10.

    Petrovska BB. Historical review of medicinal plants' usage. Pharmacogn Rev. 2012;6(11):1-5. [PubMed ID: 22654398]. https://doi.org/10.4103/0973-7847.95849.

  • 11.

    Obolskiy D, Pischel I, Feistel B, Glotov N, Heinrich M, Artemisia dracunculus L. A critical review of its traditional use, chemical composition, pharmacology and safety. J Agr Food Chem. 2011;9(17):45.

  • 12.

    Kadam PD, Chuan HH. Erratum to: Rectocutaneous fistula with transmigration of the suture: a rare delayed complication of vault fixation with the sacrospinous ligament. Int Urogynecol J. 2016;27(3):505. [PubMed ID: 26811110]. https://doi.org/10.1007/s00192-016-2952-5.

  • 13.

    Alamgir M, Uddin SJ. Recent advances on the ethnomedicinal plants as immunomodulatory agents. Ethnomedicine: a source of complementary therapeutics. 2010:227-44.

  • 14.

    Bokaeian M, Nakhaee A, Moodi B, Farhangi A, Akbarzadeh A. Effects of garlic extract treatment in normal and streptozotocin diabetic rats infected with Candida albicans. Indian J Clin Biochem. 2010;25(2):182-7. [PubMed ID: 23105907]. https://doi.org/10.1007/s12291-010-0033-y.

  • 15.

    Kullberg BJ, Netea MG, Vonk AG, van der Meer JWM. Modulation of neutrophil function in host defense against disseminated Candida albicans infection in mice. FEMS Immunology and Med Microbiol. 1999;26(3-4):299-307.

  • 16.

    Kordali S, Kotan R, Mavi A, Cakir A, Ala A, Yildirim A. Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum, and Artemisia spicigera essential oils. J Agric Food Chem. 2005;53(24):9452-8. [PubMed ID: 16302761]. https://doi.org/10.1021/jf0516538.

  • 17.

    Obistioiu D, Cristina RT, Schmerold I, Chizzola R, Stolze K, Nichita I, et al. Chemical characterization by GC-MS and in vitro activity against Candida albicans of volatile fractions prepared from Artemisia dracunculus, Artemisia abrotanum, Artemisia absinthium and Artemisia vulgaris. Chem Cent J. 2014;8(1):6. [PubMed ID: 24475951]. https://doi.org/10.1186/1752-153X-8-6.

  • 18.

    Cushnie Tim TP, Lamb AJ. Antimicrobial activity of flavonoids. Int J Antimicrobial Agents. 2005;26(5):343-56. https://doi.org/10.1016/j.ijantimicag.2005.09.002.

  • 19.

    Kalantari H, Rashidi I, Bazgir S, Dibaei A. Protective effects of hydroalcoholic extract of red grape seed (VITIS VENIFERA) in nephrotoxicity induced by amikacin in mice. Jundishapur J Natural Pharm Prod. 2007;2007(2):87-93.

  • 20.

    Pirvu L, Hlevca C, Nicu I, Bubueanu C. Comparative studies on analytical, antioxidant, and antimicrobial activities of a series of vegetal extracts prepared from eight plant species growing in Romania. J Planar Chromatography – Modern TLC. 2014;27(5):346-56. https://doi.org/10.1556/jpc.27.2014.5.4.

  • 21.

    Farahnejad Z, Ghazanfari T, Yaraee R. Immunomodulatory effects of Aloe vera and its fractions on response of macrophages against Candida albicans. Immunopharmacol immunotoxic. 2011;33(4):676-81.