APPLICATION OF FACTORIAL DESIGN FOR THE OPTIMIZED PRODUCTION OF ANTISTAPHYLOCOCCAL METABOLITE BY AUREOBASIDIUM PULLULANS

E Kalantar; R Deopurkar

Jundishapur Journal of Natural Pharmaceutical Products

The Official Journal of Ahvaz Jundishapur University of Medical Sciences

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APPLICATION OF FACTORIAL DESIGN FOR THE OPTIMIZED PRODUCTION OF ANTISTAPHYLOCOCCAL METABOLITE BY AUREOBASIDIUM PULLULANS

Author(s):

E Kalantar^1,*,

R Deopurkar²

1Department of Medical Microbiology, School of Medicine, Kurdistan University of Medical Sciences, kalantar_enayat@yahoo.com, Iran

2Department of Microbiology, University of Pune, India

Jundishapur Journal of Natural Pharmaceutical Products:Vol. 2, issue 2; 69-77

Article type:Research Article

Received:Sep 09, 2007

Accepted:Dec 08, 2007

How to Cite:E KalantarR DeopurkarAPPLICATION OF FACTORIAL DESIGN FOR THE OPTIMIZED PRODUCTION OF ANTISTAPHYLOCOCCAL METABOLITE BY AUREOBASIDIUM PULLULANS.Jundishapur J Nat Pharm Prod.2(2):69-77.

Abstract

Background: Antimicrobial substances are mainly produced by bacteria and lower fungi, and have great roles in the treatment of most infectious diseases.

Purpose: Production of antistaphylococcal metabolite from Aureobasidium pullulans by development of a cultural medium using response surface methodology. Methods: Production of antistaphylococcal metabolite from Aureobasidium pullulans was optimized in shake flasks using a statistical experimental design approach. Effect of various components in the basal medium, glucose, peptone, KH₂PO₄ as well as initial pH and temperature were statistically combined using a2 level,4 factor experimental design andtested for their influence on maximal antistaphylococcal metabolite production. Results were analyzed using response surface methodology (RSM) software. Results: Optimum production of antistaphylococcal metabolite occurred at glucose 2.0%, peptone 2.5%, KH₂PO₄0.15%, pH 4.0 and temperature 30^°C. The maximum amount of antistaphylococcal metabolite 900 U/flask from about 0.85 g of dry weight biomass was extracted. Conclusion: The antistaphylococcal activity of Aureobasidium pullulans seemed to be associated with primary metabolite rather than secondary metabolite. However, this conclusion should be taken with caution because both secondary metabolites as well as antibiotics are heterogeneous group and our knowledge regarding the exact definitions and of secondary metabolite / antibiotics are far from the perfection.

Keywords

Aureobasidium

Antistaphylococcal activity

Production

Factorial design

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