An investigation into the effect of exogenous melatonin on spermatogenesis of pinealectomised busulfan induced oligospermic adult rats

Ahad Ferdosi Khosoroshahi; Jafar Solimanirad; Seyed Behnamedin Jameie; Mehrdad Bakhtiari; Seyed Morteza  Kruji; Leila Roshangar; Mohamad Taghi Joghataei

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An investigation into the effect of exogenous melatonin on spermatogenesis of pinealectomised busulfan induced oligospermic adult rats

Author(s):

Ahad Ferdosi Khosoroshahi¹,

Jafar Solimanirad¹,

Seyed Behnamedin Jameie^2,*,

Mehrdad Bakhtiari²,

Seyed Morteza Kruji²,

Leila Roshangar¹,

Mohamad Taghi Joghataei²

1Dept. of Anatomy, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

2Dept. of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Journal of Kermanshah University of Medical Sciences:Vol. 16, issue 6; e77335

Published online:Jan 29, 2013

Article type:Research Article

Received:Jan 05, 2012

Accepted:Jun 05, 2012

How to Cite:Ahad Ferdosi KhosoroshahiJafar SolimaniradSeyed Behnamedin JameieMehrdad BakhtiariSeyed Morteza KrujiLeila RoshangarMohamad Taghi Joghataeiet al.An investigation into the effect of exogenous melatonin on spermatogenesis of pinealectomised busulfan induced oligospermic adult rats.J Kermanshah Univ Med Sci.16(6):e77335.

Abstract

Background: Damage to Spermatogenic and fertility disorders are among major side effects of chemotography drugs such as busulfan. To reduce the side effects of these drugs different methods such as protecting and fixing germinal cell epithelium, while employing chemotherapy are used. The aim of this study was to investigate the effect of exogenous melatonin on the treatment of oligospermic in spermatogenesis.

Methods: Sixty adult Wistar rats were used and randomly divided into 6 groups: 1-the control group, 2-recipient busulfan, 3-recipient of the DMSO (Vehicle busulfan) , 4-surgical sham group, 5-Pinealectomized, recipient busulfan and ethanol (Vehicle melatonin), 6-Pinealectomized, recipient busulfan and melatonin as experimental group. Single dose of busulfan (20mg/kg) and melatonin (0.5mg/kg) for 60 days were used by IP injection.

Results: Busulfan significantly reduced sperm parameters (number, motility, form and size) in comparison with that of control group (p<0.05). However, in combined treatment in comparison with recipient busulfan group, melatonin treated germinal cell epithelium and created a Spermatogenic cell mass in the seminiferous tubules.

Conclusion: Melatonin has a protective effect on spermatogenesis via unknown mechanism. It seems that decreasing oxidative damage interfere in this mechanism.

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