Effects of saffron oral gavage on protection of skin flaps against tissue necrosis and oxidative stress in rats

authors:

avatar Majid Banimohammad , avatar Mahtab Farrokhi , avatar Behnam Varshoei , avatar Seyed Abdulmajid Ayatollahi ORCID , *

Koomesh: Vol.21, issue 2; 347-353
published online: July 08, 2019
article type: Research Article
received: February 02, 2018
accepted: July 08, 2018

how to cite: Banimohammad M, Farrokhi M, Varshoei B, Ayatollahi S A. Effects of saffron oral gavage on protection of skin flaps against tissue necrosis and oxidative stress in rats. koomesh. 2019;21(2):e153078. 

Abstract

Introduction: Today, skin flaps are used in many reconstructive surgeries and healing of skin lesions as one of the main methods of treatment. Despite improvements in the fields related to enhancement of skin flap survival, flaps viability and their efficacy are still threatened with issues such as ischemia-reperfusion, oxidative stress, tissue necrosis, and even apoptosis in the affected tissue. Therefore, these threats suggest that research and development areas are needed to increase the survival rate of skin flaps and protect them from mentioned injuries, and efforts should be taken to manage or eliminate these threats. We evaluated the effect of saffron administration, before and after skin flap surgery, on tissue necrosis percentage and oxidative stress in rats. Materials and Methods: Saffron (40 mg/kg/day or 80 mg/kg/day) was administered as gavage for two weeks before and one week after flap surgery in mice. One week after surgery, the percentage of necrosis of the flap (by measuring and dividing the necrotic area over the entire length of the flap, malondialdehyde (MDA) levels and catalase (CAT) activity were measured. Results: Treatment with saffron, before and after surgery, in both doses resulted in a decrease in flap necrosis (P

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