Middle East Journal of Rehabilitation and Health
Middle East Journal of Rehabilitation and Health

article information

Middle East Journal of Rehabilitation and Health Studies: Vol.6, issue 4; e97125
published online: October 15, 2019
article type: Research Article
received: August 11, 2019
revised: September 26, 2019
accepted: September 29, 2019
DOI: https://doi.org/10.5812/mejrh.97125
Crossmark

The Role of Medical Ozone in Improving Antioxidant Status in Multiple Drug-Resistant Tuberculosis Patients: A Quasi-experimental Study

authors:

avatar Mili Arpan Shah 1 , avatar Lalit Kumar Anande 1 , avatar Amar Powar 1 , avatar Jignashah Captain 1 , avatar Pradeep Mk Nair 1 , *

Department of Research and Development, Ozone Forum of India, Mumbai, India

how to cite: Shah M A, Anande L K, Powar A, Captain J, Mk Nair P. The Role of Medical Ozone in Improving Antioxidant Status in Multiple Drug-Resistant Tuberculosis Patients: A Quasi-experimental Study. Middle East J Rehabil Health Stud. 2019;6(4):e97125. https://doi.org/10.5812/mejrh.97125.

Abstract

Background:

Multiple drug-resistant tuberculosis (MDR-TB) is a growing public health threat. Oxidative stress is reckoned as one of the rate-limiting steps in the progression of TB. Oxidative stress increases the level of tissue injury and organ damage. The use of medical ozone therapy is gaining popularity due to its anti-microbial property and its homeostatic potential.

Objectives:

The present study investigates the role of ozone therapy in boosting the antioxidant status of MDR-TB patients.

Methods:

Medical Ozone therapy was given through rectal insufflations for 21 days in seven MDR-TB patients (five males and two females) who were under anti-Koch Treatment-4 were enrolled after obtaining written consent. The sessions were spaced out at a rate of three sessions per week. Blood serum and plasma samples were collected from the patients at the baseline and at the end of the 21st session to assess the antioxidant status of superoxide dismutase (SOD) and glutathione reductase (GR) and other biochemical parameters.

Results:

Statistically significant rise was observed in SOD (P = 0.028), but not in GR (P = 0.058). No other biochemical profiles showed any statistically significant change except for a reduction in serum glutamic-pyruvic transaminase (P = 0.026). Participants also expressed subjective improvement in their mental status. However, we have not used any validated scales to record their mental status.

Conclusions:

The present study suggests that ozone can be a promising medium in tackling the oxidative stress and enhancing the positive mental attitude in ailing MDR-TB patients. Therefore, it can be considered as adjuvant therapy in treating MDR-TB. However, there are major limitations like smaller sample size and lack of control group. Large scale randomized trials are warranted.

1. Background

Tuberculosis (TB) is one of the major public health problems whose burden remains more challenging for health care experts to address (1). Recent estimates show that nearly 1.3 million deaths occurred due to TB during 2017 which is excluding an additional 300000 deaths accounted by TB in the HIV positive population, which makes TB a leading cause of mortality from an infectious disease (2). The World Health Organization (WHO) Report for Global TB Report 2018 reveals that nearly 10 million people acquired TB in 2017 (2). Though the reports suggest a mild decline in the incidence of TB (1), multi-drug resistant tuberculosis (MDR-TB) remains as a significant threat to the accelerated control efforts globally (3). Across the world 3.5% of the new cases and 18% of the previously diagnosed cases have been reported to have MDR-TB, with India remaining the country with highest percentage (24%) of MDR-TB reported (2). In a high burden country like India, the latent TB infection is more prominent than reactive TB cases (4). Further TB infections impair the lungs which makes the patient vulnerable to environmental pathogens and leaves them in an immune-compromised state (5, 6). Oxidative stress is regarded as one of the major rate-limiting steps in the progression of TB (7-9). The enhanced generation of reactive oxygen species (ROS) generated as a host defensive mechanism against mycobacterium increases tissue injury and inflammation which can lead to immunosupression (7). Using a suitable antioxidant therapy is suggested as a beneficial approach for faster recovery (7, 10-12).

Ozone, a molecule discovered in the mid-19th century, has shown to optimize anti-oxidant systems and metabolic functions of the body. The antioxidant property of ozone is attributed to its strong oxidizing property (13, 14). Thus it can restore the cellular redox balance which is impaired in infectious diseases like TB (14, 15). In 1991 Priimak et al. demonstrated that a mixture of ozone and oxygen can reduce the reproductive capacity of Mycobacterium tuberculosis which is suggestive that the use of ozone and oxygen mix can be promising intervention in the management of TB (16). Several in vivo and in vitro experiments in animals and humans advocate dissolved ozone to enhance the effect of drugs in MDR-TB (17, 18).

2. Objectives

We investigated the role of ozone therapy in boosting the anti-oxidant status of MDR-TB patients.

3. Methods

The Institutional Ethics committee of ozone forum of India approved the study protocol. This study included seven patients diagnosed with MDR-TB who were under AKT-4 (Anti Koch’s treatments) in the critical care department of a government tertiary care setting in Mumbai, India, exclusively dedicated to treating TB cases. Written consent was taken from the patients who were included in the study after explaining the role of ozone therapy and its safety considerations. All the patients were given 21 sessions of rectal ozone insufflations at a rate of 3 sessions per week. Additionally, sterile ozonized water wash was given to those patients who had emphysema surgeries.

Rectal insufflation is a method by which an ozone-oxygen mixture is administered through the rectum. The patients were asked to lie in the left lateral position to prevent any gastric discomfort and release of flatus. The interventional dose was 500 mL of ozone-oxygen mixture at 35 mcg/mL concentration; introduced into the rectum using a No. 10 infant feeding tube, up to 4” deep; after lubricating the tip of the tube with ozonated oil. Then the rectal bag was filled with ozone oxygen mixture is attached to the infant feeding tube and the ozone is dispensed slowly into the rectum by squeezing the bag gently and then rolling it circularly. The flow rate was set at 120 mL per minute to achieve the desired concentration. The procedure should be very slow and gentle to avoid any back pressure from the rectum to the bag. The whole procedure should take 3 - 4 minutes. The patient has to lie down on the back for 2 - 3 minutes for better absorption. The ozone/oxygen mixture was generated from an ozone generator for medical use (O3-Ozonics generator, Ozone Forum of India), which is automated and standardized for time, volume and concentration (19).

Blood serum and plasma samples were collected from the patients at the baseline and at the end of the 21st session to assess the anti-oxidant status of superoxide dismutase (SOD) and glutathione reductase (GR). The SODs form the first line of defense against the injuries mediated by reactive oxygen species (ROS) (20). Being an important anti-oxidant defense agent, SOD catalyzes the reaction by which superoxide anion free radical (O2-) dismutase into molecular oxygen and hydrogen peroxide (H2O2). This will decrease O2- level in the cells which is a rate-limiting step in preventing oxidative stress injury (21). In addition, GR is another potent antioxidant agent that protects the cells from oxidative damage. This is achieved by a rise in the level of reduced glutathione which neutralizes the O2- levels (22). Thus an increase in these antioxidants’ levels favors better prognosis in high oxidative stress states like TB.

Serum Superoxide dismutase was analyzed by a commercially available kit from Cayman Chemicals, USA (Cat no 706002). This assay utilized a tetrazolium salt for detection of superoxide radicals generated by xanthine oxidase and hypoxanthine. One unit of SOD is defined as the amount of enzyme needed to exhibit 50% dismutation of the superoxide radical. The SOD activity was expressed as U/mL.

Plasma Glutathione reductase assay was also performed using Cayman chemicals, USA (Cat no 703202) kits. This assay kit measured GR activity by measuring the rate of NADPH oxidation. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm and is directly proportional to the GR activity in the sample. GR activity was expressed as nmol/min/mL.

4. Results

Statistical Package for the Social Sciences (SPSS) 16.0 was used for the data analysis. A paired sample t-test was used for comparison between pre-treatment and post-treatment changes. Out of the seven participants, there were two females and five males with a mean age of 31.86 ± 9.4. Statistically, a significant rise was observed in SOD (P = 0.028), but not in GR (P = 0.058). No other biochemical profiles have shown any statistically significant change except for reduction in serum glutamic-pyruvic transaminase (P = 0.026). The results are tabulated in Table 1.

Table 1.

Pre and Post Evaluation of Ozone Therapy in MDR-TBa

Parameters InvestigatedPre-TreatmentPost-TreatmentP Value
Iron study
RBC4.47 ± 0.534.56 ± 0.290.72
HB11.72 ± 1.9112.07 ± 2.240.60
Immunity
WBC8.21 ± 1.547.74 ± 3.740.70
CRP32.03 ± 32.9834.30 ± 43.830.91
IgG549 ± 216576 ± 2100.75
IgM1.47 ± 33.021.42 ± 58.50.80
IgE3.31 ± 325.43.44 ± 374.680.90
Biochemical
Liver profile
ALP98.57 ± 30.5882.42 ± 46.170.28
Total bilirubin0.62 ± 0.170.64 ± 0.050.85
Direct bilirubin0.37 ± 0.110.32 ± 0.040.36
Indirect bilirubin0.25 ± 0.070.31 ± 0.030.17
SGPT18.14 ± 3.9713.14 ± 2.190.026b
SGOT19.14 ± 6.6620.85 ± 4.670.44
GGT39.85 ± 24.4938.85 ± 29.850.75
Protein
Total protein7.0 ± 0.487.17 ± 0.520.40
Total albumin3.67 ± 0.483.77 ± 0.460.59
Total globulin3.32 ± 0.493.4 ± 0.8320.76
Albumin/globulin ratio1.12 ± 0.281.18 ± 0.520.72
Antioxidant enzyme
SOD, U/mL1.51 ± 0.612.09 ± 1.070.028b
GR, nmol/min/mL16.88 ± 8.9621.67 ± 10.360.058

aValues are expressed as mean ± SD.

bSignificant P value.

5. Discussion

The present study demonstrates the role of ozone therapy in improving the antioxidant activity in immune-compromised conditions such as tuberculosis. The results are identical to the previous in vitro and vivo studies conducted which demonstrated a reduction in oxidative stress and enhanced antioxidant defense mechanisms post ozone interventions (14-18). Furthermore, ozone is reckoned as a strong anti-microbial agent that can destroy the cellular cavities as well as reduce the reproductive capacity of the microbial cells (16, 23). Ozone improves the cellular redox balance and increases the oxygen uptake by the cells (13, 14). This action supplements the innate immunity of the body (24). Basic science research also explains the beneficial effects of ozone therapy in destroying microorganisms including bacteria and viruses (24). MDR-TB is considered emergent problem despite the improvement of public health and medical services (25), and ozone therapy can be taken in to account as a vital complementary therapy that can enhance the potency of existing care.

Published literature suggests that the possible mechanism by which ozone therapy induces beneficial effects is by upregulating cellular antioxidant enzymes and induction of heme-oxygenase-1 (HO-1) and heat-shock proteins (HSP70) (26). These free antioxidants protect the cells from oxidation and inflammation, thereby reverse the chronic oxidative stress (27). Furthermore, it enhances the release of growth factors.

Another possible mechanism by which ozone therapy acts is by influencing the immunoglobulins (Ig). Studies have reported an increase in IgG, IgM, and IgA which is attributed to the prevention of recurrence in infections. Immunoglobulins play a large role in the humoral response of adaptive immunity, which helps in maintaining immune system homeostasis (28-31). Our study has also shown moderate increase in IgG and IgM levels. However, these changes were not statistically significant. The smaller sample size may be a reason for the statistical insignificance.

Besides the improvement in antioxidant markers, the participants expressed positive subjective changes in their mental status. However, this is based on the personal experiences shared by the patients with the investigators. No standardized scales were used to validate these psychological changes, which remains one of the drawbacks of this study. The psychological well-being post ozone therapy is attributed to the effect of ozone therapy on the neuroendocrine system (32).

Though the present study exhibits promising results, the smaller sample size is a limitation which will limit us from generalizing this result to a larger population. This further affects the effect size of the parameters under study as we have observed in GR. Lack of potential control group remains another weakness of this study. Large randomized control trials are warranted to use ozone effectively in MDR-TB.

5.1. Conclusions

In light of the current results, it is evident that ozone can be a promising medium in tackling the oxidative stress and to enhance the positive mental attitude in ailing MDR-TB patients. Medical ozone therapy can, therefore, be considered as an adjuvant in managing multidrug-resistant TB (MDR-TB).

Acknowledgements

References

  • 1.

    Sulis G, Roggi A, Matteelli A, Raviglione MC. Tuberculosis: Epidemiology and control. Mediterr J Hematol Infect Dis. 2014;6(1). e2014070. [PubMed ID: 25408856]. [PubMed Central ID: PMC4235436]. https://doi.org/10.4084/MJHID.2014.070.

  • 2.

    World Health Organization. Global Tuberculosis report 2018. 2019. Available from: https://www.who.int/tb/publications/global_report/en/.

  • 3.

    Goyal V, Kadam V, Narang P, Singh V. Prevalence of drug-resistant pulmonary tuberculosis in India: Systematic review and meta-analysis. BMC Public Health. 2017;17(1):817. [PubMed ID: 29041901]. [PubMed Central ID: PMC5645895]. https://doi.org/10.1186/s12889-017-4779-5.

  • 4.

    Pai M, Rodrigues C. Management of latent tuberculosis infection: An evidence-based approach. Lung India. 2015;32(3):205-7. [PubMed ID: 25983402]. [PubMed Central ID: PMC4429378]. https://doi.org/10.4103/0970-2113.156210.

  • 5.

    Corbett EL, Churchyard GJ, Charalambos S, Samb B, Moloi V, Clayton TC, et al. Morbidity and mortality in South African gold miners: Impact of untreated disease due to human immunodeficiency virus. Clin Infect Dis. 2002;34(9):1251-8. [PubMed ID: 11941552]. https://doi.org/10.1086/339540.

  • 6.

    Fenner L, Reid SE, Fox MP, Garone D, Wellington M, Prozesky H, et al. Tuberculosis and the risk of opportunistic infections and cancers in HIV-infected patients starting ART in Southern Africa. Trop Med Int Health. 2013;18(2):194-8. [PubMed ID: 23199369]. [PubMed Central ID: PMC3553249]. https://doi.org/10.1111/tmi.12026.

  • 7.

    Reddy YN, Murthy SV, Krishna DR, Prabhakar MC. Role of free radicals and antioxidants in tuberculosis patients. India J Tuberculosis. 2004;51:213-8.

  • 8.

    Cheresh P, Kim SJ, Tulasiram S, Kamp DW. Oxidative stress and pulmonary fibrosis. Biochim Biophys Acta. 2013;1832(7):1028-40. [PubMed ID: 23219955]. [PubMed Central ID: PMC3639303]. https://doi.org/10.1016/j.bbadis.2012.11.021.

  • 9.

    Verma I, Jindal SK, Ganguly NK. Oxidative stress in tuberculosis. Studies on Respiratory Disorders 2014. New York, NY: Humana Press; 2014. p. 101-14. https://doi.org/10.1007/978-1-4939-0497-6_6.

  • 10.

    Wiid I, Seaman T, Hoal EG, Benade AJ, Van Helden PD. Total antioxidant levels are low during active TB and rise with anti-tuberculosis therapy. IUBMB Life. 2004;56(2):101-6. [PubMed ID: 15085934]. https://doi.org/10.1080/15216540410001671259.

  • 11.

    Adebimpe WO, Faremi AO, Nassar SA. Effects of treatment on free radicals in patients with pulmonary tuberculosis in South Western Nigeria. Afr Health Sci. 2015;15(4):1256-61. [PubMed ID: 26958028]. [PubMed Central ID: PMC4765427]. https://doi.org/10.4314/ahs.v15i4.26.

  • 12.

    Bhattacharyya R, Banerjee D. Antioxidants: Friend or foe for tuberculosis patients. Adv Biosci Biotechnol. 2013;4(11):10-4. https://doi.org/10.4236/abb.2013.411A2002.

  • 13.

    Bocci V. Does ozone therapy normalize the cellular redox balance? Implications for therapy of human immunodeficiency virus infection and several other diseases. Med Hypotheses. 1996;46(2):150-4. [PubMed ID: 8692040]. https://doi.org/10.1016/s0306-9877(96)90016-x.

  • 14.

    Totolici IP, Pascu AM, Poroch V, Mosoiu D. The impact of ozone therapy on antioxidant status and quality of life in palliative care-exploratory study. Revista De Chimie. 2017;68(10):2416-21.

  • 15.

    Tusat M, Mentese A, Demir S, Alver A, Imamoglu M. Medical ozone therapy reduces oxidative stress and testicular damage in an experimental model of testicular torsion in rats. Int Braz J Urol. 2017;43(6):1160-6. [PubMed ID: 28727368]. [PubMed Central ID: PMC5734081]. https://doi.org/10.1590/S1677-5538.IBJU.2016.0546.

  • 16.

    Priimak AA, Kaliuk AN, Kirgintsev AG. [The effect of an ozone-oxygen mixture on Mycobacterium tuberculosis and conditionally pathogenic microorganisms]. Probl Tuberk. 1991;(4):7-10. Russian. [PubMed ID: 1906618].

  • 17.

    Belianin II, Abdullaev R. [Use of soluble ozone in combined treatment of pulmonary tuberculosis: lipid peroxidation and blood antioxidative defense systems]. Probl Tuberk. 2000;(3):41-4. Russian. [PubMed ID: 10900985].

  • 18.

    Guanche D, Zamora Z, Hernandez F, Mena K, Alonso Y, Roda M, et al. Effect of ozone/oxygen mixture on systemic oxidative stress and organic damage. Toxicol Mech Methods. 2010;20(1):25-30. [PubMed ID: 20017603]. https://doi.org/10.3109/15376510903503107.

  • 19.

    Zaky S, Fouad EA, Kotb HI. The effect of rectal ozone on the portal vein oxygenation and pharmacokinetics of propranolol in liver cirrhosis (a preliminary human study). Br J Clin Pharmacol. 2011;71(3):411-5. [PubMed ID: 21284700]. [PubMed Central ID: PMC3045550]. https://doi.org/10.1111/j.1365-2125.2010.03851.x.

  • 20.

    Kangralkar VA, Patil SD, Bandivadekar RM. Oxidative stress and diabetes: A review. Intl J Pharm Appl. 2010;1:38-45.

  • 21.

    Yasui K, Baba A. Therapeutic potential of superoxide dismutase (SOD) for resolution of inflammation. Inflamm Res. 2006;55(9):359-63. [PubMed ID: 17122956]. https://doi.org/10.1007/s00011-006-5195-y.

  • 22.

    Chang JC, van der Hoeven LH, Haddox CH. Glutathione reductase in the red blood cells. Ann Clin Lab Sci. 1978;8(1):23-9. [PubMed ID: 623427].

  • 23.

    Menandro ML, Martini M, Dotto G, Pasotto D. Assessment of multidrug resistant bacteria removal after environmental ozone treatment. Int J Infect Dis. 2019;79:46. https://doi.org/10.1016/j.ijid.2018.11.125.

  • 24.

    Rowen RJ. Ozone therapy as a primary and sole treatment for acute bacterial infection: Case report. Med Gas Res. 2018;8(3):121-4. [PubMed ID: 30319768]. [PubMed Central ID: PMC6178636]. https://doi.org/10.4103/2045-9912.241078.

  • 25.

    Berry M, Kon OM. Multidrug- and extensively drug-resistant tuberculosis: an emerging threat. Eur Respir Rev. 2009;18(114):195-7. [PubMed ID: 20956144]. https://doi.org/10.1183/09059180.00005209.

  • 26.

    Bocci V, Borrelli E, Travagli V, Zanardi I. The ozone paradox: Ozone is a strong oxidant as well as a medical drug. Med Res Rev. 2009;29(4):646-82. [PubMed ID: 19260079]. https://doi.org/10.1002/med.20150.

  • 27.

    Sagai M, Bocci V. Mechanisms of action involved in ozone therapy: Is healing induced via a mild oxidative stress? Med Gas Res. 2011;1:29. [PubMed ID: 22185664]. [PubMed Central ID: PMC3298518]. https://doi.org/10.1186/2045-9912-1-29.

  • 28.

    Luis JD, Padrón GS, Cepero SM, Macías Abraham C. Immunomodulator effect of ozone therapy in children with deficiency in immunity mediated by phagocytes. Mediciego. 2012;18(1).

  • 29.

    Mandzhgaladze NR, Kharebava ER, Didia Ts G, Ardzhevanishvili MD, Gudzhabidze MV, Chigiashvili Ts N. [Influence of intravenous ozone treatment on the level of different specificity antibodies]. Georgian Med News. 2006;(138):93-5. Russian. [PubMed ID: 17057311].

  • 30.

    J. Smith A, Oertle J, Warren D, Prato D. Ozone therapy: A critical physiological and diverse clinical evaluation with regard to immune modulation, anti-infectious properties, anti-cancer potential, and impact on anti-oxidant enzymes. Open J Mol Integr Physiol. 2015;5(3):37-48. https://doi.org/10.4236/ojmip.2015.53004.

  • 31.

    Díaz-Luis J, Menéndez-Cepero S, Macías-Abraham C, Fariñas-Rodríguez L. Systemic ozone therapy by rectal insufflation for Immunoglobulin A deficiency. MEDICC Rev. 2018;20:29-35.

  • 32.

    Johnson JA, Johnson DA, Kraft AD, Calkins MJ, Jakel RJ, Vargas MR, et al. The Nrf2-ARE pathway: An indicator and modulator of oxidative stress in neurodegeneration. Ann N Y Acad Sci. 2008;1147:61-9. [PubMed ID: 19076431]. [PubMed Central ID: PMC2605641]. https://doi.org/10.1196/annals.1427.036.