Abstract
An antibody-capture enzyme-linked immunosorbent assay (ELISA) based on the reaction between anti human IgM and serum IgM and HSV-1 conjugated with horseradish peroxidase (HRP) was developed. The specificity and sensitivity of the developed HSV-1 IgM ELISA were evaluated and compared with a commercially available test kit by using sets of well defined sera of known HSV-1 primary infection. For validation, 526 serum samples have been tested. The HSV-1 IgM ELISA had acceptable sensitivity and specificity, which were comparable with that of the commercial test kit. Both assays showed some cross-reactivity with sera containing anti-HSV-2 antibodies
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© 2006, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.