Abstract
Introduction: Cytomegalovirus (CMV) has been recognized as the most important viral pathogen in persons undergoing
bone marrow transplantation (BMT). In this study, we present the development of a TaqMan-based real-time PCR
assay to quantify human cytomegalovirus (CMV) DNA in peripheral blood leukocytes (PBLs) of bone marrow transplantation
patients.
bone marrow transplantation (BMT). In this study, we present the development of a TaqMan-based real-time PCR
assay to quantify human cytomegalovirus (CMV) DNA in peripheral blood leukocytes (PBLs) of bone marrow transplantation
patients.
Materials and Methods: A plasmid containing the target sequence from the pp65 region (UL83) of CMV was constructed
as a positive control template. Serial dilutions of 107 to 101 plasmids per assay were prepared. Peripheral blood
samples were collected from patients after transplantation. CMV DNA was quantified by RQ-PCR in parallel with the
pp65 antigenemia assay in PBL samples.
as a positive control template. Serial dilutions of 107 to 101 plasmids per assay were prepared. Peripheral blood
samples were collected from patients after transplantation. CMV DNA was quantified by RQ-PCR in parallel with the
pp65 antigenemia assay in PBL samples.
Results: The real-time PCR assay could detect CMV DNA in patient's samples with a wide linear range, from 10 to over
107 copies of CMV. Real-time PCR assay results correlated with those of the CMV pp65 antigenemia assay (P < 0.0001).
107 copies of CMV. Real-time PCR assay results correlated with those of the CMV pp65 antigenemia assay (P < 0.0001).
Discussion: The TaqMan assay may be a useful tool for rapid quantification of CMV infection and for monitoring of
CMV reactivation in bone marrow transplantation recipients. The results of both quantitative assays were significantly
correlated; however, the RQ-PCR assay was more sensitive than the pp65 antigenemia assay.
CMV reactivation in bone marrow transplantation recipients. The results of both quantitative assays were significantly
correlated; however, the RQ-PCR assay was more sensitive than the pp65 antigenemia assay.
Keywords
Cytomegalovirus TaqMan PCR pp65 Antigenemia Bone Marrow Transplantation
Fulltext
References
-
1.
The references are available in the PDF file.
Copyright
© 2006, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.