Evaluation of Relative Potency of Human Tuberculin Skin Test in the Guinea Pigs Sensitized with Mycobacterium Tuberculosis, M. Bovis BCG and M. Avium

Davoud Sadeghi-Gariz; Nader Mosavari; Behnam Rafiee; Shojaat Dashtipour; Ahmad Sakhravi; Emran Heshmati; Abbas Zare

Zahedan Journal of Research in Medical Sciences

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Evaluation of Relative Potency of Human Tuberculin Skin Test in the Guinea Pigs Sensitized with Mycobacterium Tuberculosis, M. Bovis BCG and M. Avium

Author(s):

Davoud Sadeghi-Gariz¹,

Nader Mosavari^2,*,

Behnam Rafiee³,

Shojaat Dashtipour²,

Ahmad Sakhravi²,

Emran Heshmati¹,

Abbas Zare²

1Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2Razi Vaccine and Serum Research Institute, Karaj, Iran

3Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran

Zahedan Journal of Research in Medical Sciences:Vol. 15, issue 12; e4324

Published online:Apr 15, 2013

Article type:Research Article

Received:Apr 17, 2012

Accepted:Jul 05, 2012

How to Cite:Davoud Sadeghi-GarizNader MosavariBehnam RafieeShojaat DashtipourAhmad SakhraviEmran HeshmatiAbbas Zareet al.Evaluation of Relative Potency of Human Tuberculin Skin Test in the Guinea Pigs Sensitized with Mycobacterium Tuberculosis, M. Bovis BCG and M. Avium.Zahedan J Res Med Sci.15(12):e4324.

Abstract

Background: The tuberculin skin test is the most commonly used test for diagnosing tuberculosis (TB) infection. The basis of tuberculin testing is the induction of a delayed hypersensitivity reaction to the intradermal injection of tuberculin. Unfortunately, this test is incapable of distinguishing Mycobacterium tuberculosis infection from Bacille Calmette-Guerin (BCG) vaccination or infection with non-tuberculous mycobacteria. The aim of this study is to evaluate the relative potency of human tuberculin skin test )produced by Razi Vaccine and Serum Research Institute( in the guinea pigs sensitized with M. tuberculosis, M. bovis BCG and M. avium.

Materials and Methods: For skin test, different groups of guinea pigs were sensitized with M. tuberculosis, M. avium and M. bovis BCG. Guinea pigs were injected intradermally with 0.1 ml of 0.4, 2 and 10 µg/ml of tuberculin. Skin reactions (diameters of erythema, in millimeters) were independently measured 24 h after injection and results were calculated.

Results: The results showed that the specificity index of human tuberculin test for guinea pigs sensitized with M. bovis BCG in compare of guinea pigs sensitized with M. tuberculosis was equal and for guinea pigs sensitized with M. avium was not equal.

Conclusion: This study demonstrated that human tuberculin test produced by Razi Institute for diagnosis of latent infection to M. tuberculosis has lower specificity for M. bovis in comparison with M. avium

Keywords

Human tuberculin skin test

Mycobacterium tuberculosis

Mycobacterium avium

Mycobacterium bovis BCG

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