The effects of concurrent use of vitamin A and very low frequency electromagnetic field (50Hz) on limb bud development in chick embryo

Javad  Baharara; Masomeh Saboori

Zahedan Journal of Research in Medical Sciences

The Official Journal of Zahedan University of Medical Sciences

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The effects of concurrent use of vitamin A and very low frequency electromagnetic field (50Hz) on limb bud development in chick embryo

Author(s):

Javad Baharara^1,*,

Masomeh Saboori²

1Assistant Professor of Biology, School of Basic Sciences, Islamic Azad University Mashhad branch, Mashhad, Iran.

2Msc of Cell & Developmental Biology, School of Basic Sciences, Islamic Azad University Mashhad Branch, Mashhad, Iran.

Zahedan Journal of Research in Medical Sciences:Vol. 13, issue 2; e94059

Published online:Aug 19, 2010

Article type:Research Article

Received:May 11, 2010

Accepted:Jun 15, 2010

How to Cite:Javad BahararaMasomeh SabooriThe effects of concurrent use of vitamin A and very low frequency electromagnetic field (50Hz) on limb bud development in chick embryo.Zahedan J Res Med Sci.13(2):e94059.

Abstract

Background: Electromagnetic fields depending on the intensity and frequency have variety effects on living organisms. Vitamin A has an important role on the growth of living organisms. In this study, the effects of concurrent use of vitamin A and very low frequency electromagnetic field on the growth of limb bud of chick embryo have been investigated.
Materials and Method: This experimental laboratory study was performed on 30 eggs with Gallus gallus fertilized race. The eggs were randomly divided into three equal groups including control, laboratory control and experimental. The samples of experimental group were treated with vitamin A 56 hours after incubation (1 IU). Then eggs were placed in active electromagnetic waves generator machine with 50 HZ frequency and 50 G intensity for 3 hours. On the twelfth day of incubation, embryos were removed from their eggs and measured embryo weight and total length of embryo hindlimb. Then embryos were prepared for histological studies with microscope using measurement software "Image J" in microscope sections at length of tibia, length of ontogenesis zone and also counting of chondrocytes of epiphysis and diaphysis. The resulting data were analyzed with SPSS-15.
Results: The results show a significant decrease in the average of embryo weight, total length of embryo limb, length of tibia, average of chondrocyte zones of epiphysis and diaphysis and average length of osteogenesis zone (p=0.001).
Conclusion: Based on this Study we conclude that concurrent use of vitamin A and very low frequency electromagnetic field has a delayed effect on development of chick embryo and it causes decreased embryo weight, limb length and accumulation of chondrocyte

Keywords

Electromagnetic fields

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