Antioxidant effect of extract of the grape seed in streptozotocin induced diabetic rats

Yousef Doustar; Daryoush Mohajeri

Zahedan Journal of Research in Medical Sciences

The Official Journal of Zahedan University of Medical Sciences

Current Issue All Issues In Press Articles Accepted Manuscripts Search

Journal Information Boards and Committees Indexing and Listing Sources Journal Metrics Open Peer Review (OPR)Publication Ethics and Malpractice Statement Contact Us Support Reviewer and AE Registration Form

APC

Authors Guide Submit Manuscript

Antioxidant effect of extract of the grape seed in streptozotocin induced diabetic rats

Author(s):

Yousef Doustar^1,*,

Daryoush Mohajeri²

1Assistant Professor of Pathology, Dept. of Pathobiology, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

2Associate Professor of Pathology, Dept. of Pathobiology, School of Veterinary Medicine, Islamic Azad University, Tabriz Branch, Tabriz, Iran.

Zahedan Journal of Research in Medical Sciences:Vol. 12, issue 1; e94345

Published online:Jan 23, 2010

Article type:Research Article

Received:Oct 18, 2009

Accepted:Dec 12, 2009

How to Cite:Yousef DoustarDaryoush MohajeriAntioxidant effect of extract of the grape seed in streptozotocin induced diabetic rats.Zahedan J Res Med Sci.12(1):e94345.

Abstract

Background: Diabetes mellitus is a metabolic disorder as old as mankind and its incidence is considered to be high all over the world. Oxidative stress is strongly associated with development and the complications of diabetes. Antioxidant agents, especially with the origin of plants, are of more importance in the treatment of diabetic complications. The main objective of the present study was to evaluate the effect of extract of the grape seed on antioxidants status in streptozotocin induced diabetic rats.

Material and methods: In this laboratory experimental study which conducted in Islamic Azad University of Tabriz research center. Forty male Wistar rats were randomly assigned into four equal groups including healthy control group, healthy group treated with grape seed extract (40 mg/kg), diabetic control group and diabetic group treated with grape seed extract (40 mg/kg). The experimental rats were treated in related groups for 12 weeks and at the end of experiment serum level of malonaldehyde (MDA) and anti-oxidant enzymes activity including superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase (CAT) were measured in red blood cells. Statistically, comparison of the groups was carried out using one-way analysis of variance followed by Bonferroni post-hoc test. Differences were considered statistically significant at p< 0.05.

Results: Diabetic rats showed significant increase in the value of MDA and a decrease in the activities of SOD, GPx and CAT of red blood cells (p<0.001). Oral administration of grape seed extract resulted in significant reduction in the level of MDA and significant increase in the activities of SOD, GPx and CAT of red blood cells (p<0.001).

Conclusion: The results of this study provide confirmatory evidence of oxidative stress in diabetes and show the anti-oxidative effect of grape seed extract.

Keywords

Fulltext

The full text of this article is available on the PDF file.

References

1.
The References of this article are available on the PDF file.

comments