Logo
homeHome
toggle_offCompany ProfilegroupsBoards & Staffsworkspace_premiumMemberships & PartnersschoolAcademy of Journalismperson_addCareerslinkBlog(EN)emailContact Us
list_altOur JournalslightbulbInstruction for AuthorsbookmarkBrieflands PolicieshandshakePublish My Researchplay_circleJournal Management Systemcredit_cardArticle Processing Chargeszoom_inOpen Peer ReviewimagePublishing Services
menu_bookPublish My Book

Archives of Clinical Infectious Diseases

Infectious Diseases and Tropical Medicine Research Center, SBUMS

homeHome
play_arrowCurrent Issuecheck_circleAccepted ManuscriptslistAll Issuesformat_indent_increaseIn PresssearchSearch
settingsInstructions
bookmarkJournal InformationgroupsEditors & BoardsshareIndexing and Listing Sourcesshow_chartJournal MetricsbalancePublication Ethics and Malpractice StatementemailSupportphoneContact Usassignment_indReviewer and AE Registration Formzoom_inOpen Peer Review (OPR)
format_list_bulletedAPC
Authors GuideSubmit Manuscript

Outlines

Identification of Infective Larva (L3) Proteins of Strongyloides Stercoralis by Immunoblot

Author(s):
Soheila RouhaniSoheila Rouhani1,*, Mehri MahmoudiMehri Mahmoudi1
1Department of Parasitology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences., Tehran, IR Iran
Archives of Clinical Infectious Diseases:Vol. 5, issue 2; 101-5
Published online:Apr 30, 2010
Article type:Brief Report
How to Cite:Rouhani S, Mahmoudi M. Identification of Infective Larva (L3) Proteins of Strongyloides Stercoralis by Immunoblot. Arch Clin Infect Dis. 2010;5(2):. doi:

Abstract

Background:

Strongyloides stercoralis is prevalent in tropical and subtropical regions worldwide. This parasite is the only nematode with the ability to multiply in its host's body via autoinfection transmission. Larvae detection in feces is difficult partly because of low egg production and also irregular larvae excretion in feces. Serologic tests (ELISA, IFA) are also diagnostic, however Strongyloides stercoralis antigens are not available as a diagnostic tool. In the present study, we analyzed filariform larva (L3) proteins of Strongyloides stercoralis by the immunoblot technique.

Materials and Methods:

Stool samples were examined by direct smear, formalin-ether and agar plate method to identify infected patients. Sera were also obtained and stored at -20?. Infective larvae were then obtained by agar plate culture, which was incubated for 6-7days at 25C, then frozen at 70?. Finally, larvae were suspended at a concentration level of 12000 in 250?l PBS, containing protease inhibitors and then were sonicated. Protein level was measured by Bradford method. Proteins of Strongyloides stercoralis filariform larvae were separated by SDS-PAGE, blotted onto nitrocellulose paper. Western blot analysis of these antigens was achieved using infected human sera (0.1, 0.01, 0.001 dilution) with strongyloidiasis, toxocariasis, hydatidosis, amebiasis and normal human serum as control.

Results:

Four immunodominant proteins (23, 28, 30, 41 kDa) were recognized with strongyloidiasis sera in 0.1 diluted serum. None of the proteins reacted to normal human and amebiasis serum, but some showed reaction with serum of hydatidosis and toxocariasis. Having increased the level of serum dilution, only 41 kDa protein was recognized by strongyloidiasis sera. Other serums did not represent any reaction to the parasites proteins. Therefore, the 41 kDa protein presents as the most important immunodominant protein in this study.

Conclusion:

The identification of immunodominant proteins adapted to the physiological and genetic conditions of the host is an appropriate diagnostic approach, which could be associated with improved sensitivity and specificity of serologic tests.

Keywords
Strongyloides stercoralis
Infective larva (L3)
SDS-PAGE
Immunoblot

Full Text

Full text is available in PDF

Similar Articles

31
Mar
2020

Parasitological, Immunological, and Molecular Methods in Diagnosis of Human Strongyloidiasis

ZOHREH fakhrieh kashan,
Meysam Sharifdini

fakhrieh kashan Z, Sharifdini M. Parasitological, Immunological, and Molecular Methods in Diagnosis of Human Strongyloidiasis. J Inflamm Dis. 2024;23(6):e156195. doi:

25
Jan
2014

Epidemiological Study of Strongyloides stercoralis With a Comparative Diagnostic Approach, in Lorestan, West of Iran

Ebrahim Badparva,
Hassan Nayebzadeh,
Malek Hossein Barkhordari,
Behrouz Ezatpour

Badparva E, Nayebzadeh H, Barkhordari MH, Ezatpour B. Epidemiological Study of Strongyloides stercoralis With a Comparative Diagnostic Approach, in Lorestan, West of Iran. Arch Clin Infect Dis. 2014;9(2):e16815. doi: https://doi.org/10.5812/archcid.16815

23
Jan
2016

Strongyloides stercoralis in Immunosuppressed Patients

Marcelo Corti

Corti M. Strongyloides stercoralis in Immunosuppressed Patients. Arch Clin Infect Dis. 2016;11(1):e27510. doi: https://doi.org/10.5812/archcid.27510

6
Dec
2021
Arch Clin Infect Dis

The Importance of Screening Strongyloides stercoralis Infection in Solid Organ Transplant Donors and Recipients

Sara Abolghasemi,
Farnaz Zolfaghari,
Shahnaz Sali,
Abdollah Ghaffari,
Mehran Noori

Abolghasemi S, Zolfaghari F, Sali S, Ghaffari A, Noori M. The Importance of Screening Strongyloides stercoralis Infection in Solid Organ Transplant Donors and Recipients. Arch Clin Infect Dis. 2021;16(4):e118047. doi: https://doi.org/10.5812/archcid.118047

14
Aug
2019
Archives of Clinical Infectious Diseases

Prevalence and Associated Risk Factors of Strongyloides sp. Infection in Diabetic Patients in the Central Part of Mazandaran, Northern Iran

Narges Kalantari,
Zeinab Darbandi,
Mohamad Ali Bayani,
Mitra Sharbatkhori,
Masomeh Bayani,
Salman Ghaffari

Kalantari N, Darbandi Z, Bayani MA, Sharbatkhori M, Bayani M, et al. Prevalence and Associated Risk Factors of Strongyloides sp. Infection in Diabetic Patients in the Central Part of Mazandaran, Northern Iran. Arch Clin Infect Dis. 2019;14(4):e86569. doi: https://doi.org/10.5812/archcid.86569

Import into EndNoteImport into BibTex
Share on
Comments
Number of Comments:0
Cited by
Metrics

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC 

Search Relations

Author(s):

Related Articles

Related Article in PubMed

Related Article in Google Scholar

Create Citation Alert via Google Reader