Archives of Clinical Infectious Diseases
Infectious Diseases and Tropical Medicine Research Center, SBUMS
Authors
Trichomonas vaginalis infection is the most prevalent sexually transmitted disease in the world. It causes vaginitis, urethritis and preterm birth. It has been associated with nongonococcal urethritis in men. In this study, a polymerase chain reaction (PCR) targeting the beta-tubulin genes of T. vaginalis was developed for the detection of the organism in both vaginal swab and urine specimens from infected patients.
Random urine samples were collected from 30 patients (23 females and 7 males) tested positive for T. vaginalis by wet preparation and the Inpouch T. vaginalis culture system. Two vaginal swabs were collected by each woman, first before insertion of the speculum and then after the insertion of the speculum. A previously published T.vaginalis specific primer set, (BTUB 9/2), BTUB 9 (5' CAT TGA TAA CGA AGC TCT TTA CGA T 3') and BTUB2 (5' GCA TGT TGT GCC GGA CAT AAC CAT 3') recognizing a 112-bp target within the ?- tubulin gene of the T. vaginalis organism was used for this purpose.
The positive result was reported 28.6% in male urine and 39.1% in female urine samples, first swab 65.2% and second swab 78.3% by wet preparation diagnosis. By the culture test, the male urine samples recorded 42.9% positive, female urine 69.6% while the first swab recorded 86.9% positive and the second swab 91.3% positive. All negative cases by culture in urine and vaginal samples were tested by PCR, which resulted as 2 cases positive in male urine samples and 5 cases were positive in female urine samples, but one case only gave positive with PCR in first swab of vaginal samples and 2 cases of second swab became positive by PCR. No statistical differences were observed in incidences among patients
On conclusion the PCR assay was even more sensitive than wet preparation and culture and afforded the practical advantages of providing results.
© 2006, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Hashemi H, Shakibapour M, Fazeli H, Varshowsaz J, Aliyari Z, et al. Molecular Study of Gardnerella vaginalis Isolated from Vaginal Discharge of Women Referring to Gynecology Clinics in Chaharmahal and Bakhtiari, Iran, in 2017. Arch Clin Infect Dis. 2021;16(2):e92537. doi: https://doi.org/10.5812/archcid.92537
Dehghan Haghighi J, Jafarimodrek M, Sohrabi S, Azizi H, Hatam-Nahavandi K. Trichomoniasis Prevalence at a Care Center Among Women with High-Risk Behaviors in Zahedan, Iran. Int J High Risk Behav Addict. 2019;8(2):e86887. doi: https://doi.org/10.5812/ijhrba.86887
Nourian A, Shabani N, Fazaeli A, Mousavinasab SN. Prevalence of Trichomonas vaginalis in Pregnant Women in Zanjan, Northwest of Iran. Jundishapur J Microbiol. 2013;6(8):e7258. doi: https://doi.org/10.5812/jjm.7258
Zangiabadi M, Iqbal-Qureshi M, Khoushideh M, Roudbari M, Bahrami S. Survey of Sensitivity of Wet smear and Dorset medium in comparison with Diamond medium for Diagnosis of Trichomonas vaginalis. Zahedan J Res Med Sci. 2002;4(3):e95325. doi:
Mirahmadi H, Metanat Y, Azizi SG, Farzaneh F, Metanat M, et al. Frequency of Trichomonas vaginalis in Pap Smears of Women Referring to the Gynecology Clinics in Zahedan from 2013 to 2022. Int J Infect. 2024;11(2):e159802. doi: https://doi.org/10.5812/iji-159802
Last Update: 1 month ago
Last Update: 1 month ago
Last Update: 1 month ago
Last Update: 6 days ago
Last Update: 3 hours ago
Last Update: 3 days ago