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Journal of Semnan University of Medical Sciences

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Cover image of the article: The Effect of Resistance Exercise and Sumac Intake on Serum Levels of Interleukin1- Beta and Nitric Oxide in Male Rats with Alzheimer's Disease

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Outline

https://doi.org/10.69107/koomesh-149432

The Effect of Resistance Exercise and Sumac Intake on Serum Levels of Interleukin1- Beta and Nitric Oxide in Male Rats with Alzheimer's Disease

Authors

Azadeh Naderi1, Abbas SaremiAbbas Saremi ORCID2,*, Mohammad Reza Afarinesh3
1Department of Physical Education, Borujerd Branch, Islamic Azad University, Borujerd, Iran
2Department of Sport Physiology, Faculty of Sport Sciences, Arak University, Arak. Iran
3Neuroscience Research Center, Kerman University of Medical Sciences, Kerman, Iran
*Corresponding Author: Department of Sports Science, Faculty of Physical Education, Arak University, Arak, Iran. Email: a-saremi@araku.ac.ir
Koomesh:Vol. 27, issue 5; e149432
Published online:Sep 16, 2025
Article type:Research Article
How to Cite:Naderi A, Saremi A, Afarinesh MR. The Effect of Resistance Exercise and Sumac Intake on Serum Levels of Interleukin1- Beta and Nitric Oxide in Male Rats with Alzheimer's Disease. Koomesh. 2025;27(5):e149432. doi: https://doi.org/10.69107/koomesh-149432

Abstract

Background: Alzheimer's is one of the diseases related to old age, which is associated with dementia and cognitive problems in
patients. Utilizing safe and low-cost treatment approaches, such as exercise and natural antioxidants, can be a beneficial way to
manage this disease.
Objectives: Therefore, the purpose of this study is to investigate the effect of resistance exercise and receiving sumac on the level
of interleukin1- beta and nitric oxide in Alzheimer's rats.
Methods: In an experimental study, 28 Wistar rats (aged 8 - 10 weeks and with an average weight of 230 ± 30 grams) were selected
and randomly divided into four groups of seven: Alzheimer's control, Alzheimer's induced by resistance exercise, Alzheimer's
resistant + consumption of sumac, and Alzheimer's induced by sumac. 10% of sumac powder was used in rats' food (10 mL/kg). To
induce Alzheimer's in mice, an amount of 8 mg/kg of body weight of trimethyltin chloride was used, along with 200 microliters of
Thermal saline as a solvent was injected intraperitoneally into the mice. Resistance training was carried out for 12 weeks, 5 days a
week, in the form of climbing with weights attached to the mice's tails from a one-meter-long ladder with 26 steps and an angle of
85 degrees. 48 hours after the last intervention session, serum levels of nitric oxide and interleukin1- beta (IL1B) were measured by
the ELISA method.
Results: The results of this study showed that the consumption of sumac (P < 0.0001, F = 28.157) decreases the amount of interleukin1-
beta. (F = 113.7349, P < 0.0001) and consumption of sumac (P < 0.0001, F = 21.730) was significant in increasing nitric oxide.
Conclusion: The results suggest that resistance exercise and receiving sumac are effective in improving inflammatory factors in
Alzheimer's disease, and combining these two interventions leads to a doubling of the effect.

Highlights

KeywordsAlzheimer DiseaseResistance TrainingsumacNitric OxideIL1B Protein

References

  • 1.
    Breijyeh Z, Karaman R. Comprehensive Review on Alzheimer's Disease: Causes and Treatment. Molecules. 2020;25(24). [PubMed ID:33302541]. [PubMed Central ID:PMC7764106]. https://doi.org/10.3390/molecules25245789.
  • 2.
    Shabab T, Khanabdali R, Moghadamtousi SZ, Kadir HA, Mohan G. Neuroinflammation pathways: a general review. Int J Neurosci. 2017;127(7):624-33. [PubMed ID:27412492]. https://doi.org/10.1080/00207454.2016.1212854.
  • 3.
    Basiri A, Izadi S, Kakhki S, Alikahni V, Askarian S, Beheshti F. N‑acetylcysteine prevents hypothyroidism‑induced impairment of learning and memory in adolescent male rats via affecting oxidative status, inflammatory response and BDNF in hippocampal tissues. Acta Neurobiol Exp (Wars). 2024;84(2):218-29. [PubMed ID:39087834]. https://doi.org/10.55782/ane-2024-2587.
  • 4.
    R AA. Risk factors for Alzheimer's disease. Folia Neuropathol. 2019;57(2):87-105. [PubMed ID:31556570]. https://doi.org/10.5114/fn.2019.85929.
  • 5.
    Bai R, Guo J, Ye XY, Xie Y, Xie T. Oxidative stress: The core pathogenesis and mechanism of Alzheimer's disease. Ageing Res Rev. 2022;77:101619. [PubMed ID:35395415]. https://doi.org/10.1016/j.arr.2022.101619.
  • 6.
    Azargoonjahromi A. Dual role of nitric oxide in Alzheimer's disease. Nitric Oxide. 2023;134-135:23-37. [PubMed ID:37019299]. https://doi.org/10.1016/j.niox.2023.03.003.
  • 7.
    Dubey H, Gulati K, Ray A. Alzheimer's Disease: A Contextual Link with Nitric Oxide Synthase. Curr Mol Med. 2020;20(7):505-15. [PubMed ID:31782366]. https://doi.org/10.2174/1566524019666191129103117.
  • 8.
    Rudrapal M, Khairnar SJ, Khan J, Dukhyil AB, Ansari MA, Alomary MN, et al. Dietary Polyphenols and Their Role in Oxidative Stress-Induced Human Diseases: Insights Into Protective Effects, Antioxidant Potentials and Mechanism(s) of Action. Front Pharmacol. 2022;13:806470. [PubMed ID:35237163]. [PubMed Central ID:PMC8882865]. https://doi.org/10.3389/fphar.2022.806470.
  • 9.
    Salisbury D, Bronas U. Reactive oxygen and nitrogen species: impact on endothelial dysfunction. Nurs Res. 2015;64(1):53-66. [PubMed ID:25502061]. https://doi.org/10.1097/NNR.0000000000000068.
  • 10.
    Khalilpour S, Behnammanesh G, Suede F, Ezzat MO, Muniandy J, Tabana Y, et al. Neuroprotective and Anti-Inflammatory Effects of Rhus coriaria Extract in a Mouse Model of Ischemic Optic Neuropathy. Biomedicines. 2018;6(2). [PubMed ID:29690612]. [PubMed Central ID:PMC6027176]. https://doi.org/10.3390/biomedicines6020048.
  • 11.
    Hillman CH, Erickson KI, Kramer AF. Be smart, exercise your heart: exercise effects on brain and cognition. Nat Rev Neurosci. 2008;9(1):58-65. [PubMed ID:18094706]. https://doi.org/10.1038/nrn2298.
  • 12.
    Kennedy G, Hardman RJ, Macpherson H, Scholey AB, Pipingas A. How Does Exercise Reduce the Rate of Age-Associated Cognitive Decline? A Review of Potential Mechanisms. J Alzheimers Dis. 2017;55(1):1-18. [PubMed ID:27636853]. https://doi.org/10.3233/JAD-160665.
  • 13.
    Alsubaie SF, Alkathiry AA, Abdelbasset WK, Nambi G. The Physical Activity Type Most Related to Cognitive Function and Quality of Life. Biomed Res Int. 2020;2020:8856284. [PubMed ID:33381590]. [PubMed Central ID:PMC7765724]. https://doi.org/10.1155/2020/8856284.
  • 14.
    Basso JC, Suzuki WA. The Effects of Acute Exercise on Mood, Cognition, Neurophysiology, and Neurochemical Pathways: A Review. Brain Plast. 2017;2(2):127-52. [PubMed ID:29765853]. [PubMed Central ID:PMC5928534]. https://doi.org/10.3233/BPL-160040.
  • 15.
    Loriette C, Ziane C, Ben Hamed S. Neurofeedback for cognitive enhancement and intervention and brain plasticity. Rev Neurol (Paris). 2021;177(9):1133-44. [PubMed ID:34674879]. https://doi.org/10.1016/j.neurol.2021.08.004.
  • 16.
    Philippe AG, Py G, Favier FB, Sanchez AM, Bonnieu A, Busso T, et al. Modeling the responses to resistance training in an animal experiment study. Biomed Res Int. 2015;2015:914860. [PubMed ID:25695093]. [PubMed Central ID:PMC4324815]. https://doi.org/10.1155/2015/914860.
  • 17.
    Nagib R. Hypolipidemic Effect of Sumac (Rhus Coriaria L) Fruit Powder and Extract on Rats Fed High Cholesterol Diet. Bulletin National Nutrition Institute Arab Republic Egypt. 2017;50(1):119-43. https://doi.org/10.21608/bnni.2017.6726.
  • 18.
    Kjolhede T, Dalgas U, Gade AB, Bjerre M, Stenager E, Petersen T, et al. Acute and chronic cytokine responses to resistance exercise and training in people with multiple sclerosis. Scand J Med Sci Sports. 2016;26(7):824-34. [PubMed ID:26105554]. https://doi.org/10.1111/sms.12504.
  • 19.
    Tsai CL, Pai MC, Ukropec J, Ukropcova B. Distinctive Effects of Aerobic and Resistance Exercise Modes on Neurocognitive and Biochemical Changes in Individuals with Mild Cognitive Impairment. Curr Alzheimer Res. 2019;16(4):316-32. [PubMed ID:30819077]. https://doi.org/10.2174/1567205016666190228125429.
  • 20.
    Moradian H, Hosseinpour Delavar S, Zabet A. [The effects of eight weeks circuit resistance training on some endothelial markers, blood pressure and lipid profiles in pre-hypertensive obese women]. J Sport Exercise Physiol. 2022;15(2):84-94. Persian. https://doi.org/10.52547/joeppa.15.2.84.
  • 21.
    Hashiguchi D, Campos HC, Wuo-Silva R, Faber J, Gomes da Silva S, Coppi AA, et al. Resistance Exercise Decreases Amyloid Load and Modulates Inflammatory Responses in the APP/PS1 Mouse Model for Alzheimer's Disease. J Alzheimers Dis. 2020;73(4):1525-39. [PubMed ID:31958083]. https://doi.org/10.3233/JAD-190729.
  • 22.
    Liu Y, Chu JMT, Yan T, Zhang Y, Chen Y, Chang RCC, et al. Short-term resistance exercise inhibits neuroinflammation and attenuates neuropathological changes in 3xTg Alzheimer's disease mice. J Neuroinflammation. 2020;17(1):4. [PubMed ID:31900170]. [PubMed Central ID:PMC6942350]. https://doi.org/10.1186/s12974-019-1653-7.
  • 23.
    Kametani F, Hasegawa M. Reconsideration of Amyloid Hypothesis and Tau Hypothesis in Alzheimer's Disease. Front Neurosci. 2018;12:25. [PubMed ID:29440986]. [PubMed Central ID:PMC5797629]. https://doi.org/10.3389/fnins.2018.00025.
  • 24.
    Heneka MT, Carson MJ, El Khoury J, Landreth GE, Brosseron F, Feinstein DL, et al. Neuroinflammation in Alzheimer's disease. Lancet Neurol. 2015;14(4):388-405. [PubMed ID:25792098]. [PubMed Central ID:PMC5909703]. https://doi.org/10.1016/S1474-4422(15)70016-5.
  • 25.
    De Sousa RAL, Rodrigues CM, Mendes BF, Improta-Caria AC, Peixoto MFD, Cassilhas RC. Physical exercise protocols in animal models of Alzheimer's disease: a systematic review. Metab Brain Dis. 2021;36(1):85-95. [PubMed ID:33095371]. https://doi.org/10.1007/s11011-020-00633-z.
  • 26.
    de Almeida EJR, Ibrahim HJ, Chitolina Schetinger MR, de Andrade CM, Cardoso AM. Modulation of Inflammatory Mediators and Microglial Activation Through Physical Exercise in Alzheimer's and Parkinson's Diseases. Neurochem Res. 2022;47(11):3221-40. [PubMed ID:35962936]. https://doi.org/10.1007/s11064-022-03713-x.
  • 27.
    Li MH, Xiao R, Li JB, Zhu Q. Regenerative approaches for cartilage repair in the treatment of osteoarthritis. Osteoarthritis Cartilage. 2017;25(10):1577-87. [PubMed ID:28705606]. https://doi.org/10.1016/j.joca.2017.07.004.
  • 28.
    Bronzuoli MR, Iacomino A, Steardo L, Scuderi C. Targeting neuroinflammation in Alzheimer's disease. J Inflamm Res. 2016;9:199-208. [PubMed ID:27843334]. [PubMed Central ID:PMC5098782]. https://doi.org/10.2147/JIR.S86958.
  • 29.
    Zaros PR, Pires CE, Bacci M, Moraes C, Zanesco A. Effect of 6-months of physical exercise on the nitrate/nitrite levels in hypertensive postmenopausal women. BMC Womens Health. 2009;9:17. [PubMed ID:19545388]. [PubMed Central ID:PMC2714506]. https://doi.org/10.1186/1472-6874-9-17.
  • 30.
    Song W, Kwak HB, Kim JH, Lawler JM. Exercise training modulates the nitric oxide synthase profile in skeletal muscle from old rats. J Gerontol A Biol Sci Med Sci. 2009;64(5):540-9. [PubMed ID:19304939]. [PubMed Central ID:PMC2800810]. https://doi.org/10.1093/gerona/glp021.
  • 31.
    Behjati Ardakani A, Qassemian A, Koushki M, Shakour E, Mehrez A. [The Effect of a Resistance Training Course on Blood Pressure and Nitric Oxide Levels in Elderly Women]. Salmand: Iran J Ageing. 2018;13(1):16-27. Persian. https://doi.org/10.21859/sija.13.1.16.
  • 32.
    Guzel NA, Hazar S, Erbas D. Effects of different resistance exercise protocols on nitric oxide, lipid peroxidation and creatine kinase activity in sedentary males. J Sports Sci Med. 2007;6(4):417-22. [PubMed ID:24149472]. [PubMed Central ID:PMC3794479].
  • 33.
    Jensen CS, Bahl JM, Ostergaard LB, Hogh P, Wermuth L, Heslegrave A, et al. Exercise as a potential modulator of inflammation in patients with Alzheimer's disease measured in cerebrospinal fluid and plasma. Exp Gerontol. 2019;121:91-8. [PubMed ID:30980923]. https://doi.org/10.1016/j.exger.2019.04.003.
  • 34.
    Barrientos RM, Frank MG, Crysdale NY, Chapman TR, Ahrendsen JT, Day HE, et al. Little exercise, big effects: reversing aging and infection-induced memory deficits, and underlying processes. J Neurosci. 2011;31(32):11578-86. [PubMed ID:21832188]. [PubMed Central ID:PMC3171131]. https://doi.org/10.1523/JNEUROSCI.2266-11.2011.
  • 35.
    Ozbeyli D, Sari G, Ozkan N, Karademir B, Yuksel M, Cilingir Kaya OT, et al. Protective effects of different exercise modalities in an Alzheimer's disease-like model. Behav Brain Res. 2017;328:159-77. [PubMed ID:28390878]. https://doi.org/10.1016/j.bbr.2017.03.044.
  • 36.
    Bozoki A, Giordani B, Heidebrink JL, Berent S, Foster NL. Mild cognitive impairments predict dementia in nondemented elderly patients with memory loss. Arch Neurol. 2001;58(3):411-6. [PubMed ID:11255444]. https://doi.org/10.1001/archneur.58.3.411.
  • 37.
    Eskandari Z, Mirzaei B, Arazi H. [Comparison of an aerobic training course with a herbal supplementation (Valeriana wallichii and Melissa officinalis) regarding serotonin levels, nitric oxide and headache indices in women with migraine]. Daneshvar Med. 2018;26(2):51-64. Persian.
  • 38.
    Pourahmad J, Eskandari MR, Shakibaei R, Kamalinejad M. A search for hepatoprotective activity of aqueous extract of Rhus coriaria L. against oxidative stress cytotoxicity. Food Chem Toxicol. 2010;48(3):854-8. [PubMed ID:20036300]. https://doi.org/10.1016/j.fct.2009.12.021.
  • 39.
    Mansouri MT, Naghizadeh B, Ghorbanzadeh B, Farbood Y, Sarkaki A, Bavarsad K. Gallic acid prevents memory deficits and oxidative stress induced by intracerebroventricular injection of streptozotocin in rats. Pharmacol Biochem Behav. 2013;111:90-6. [PubMed ID:24036471]. https://doi.org/10.1016/j.pbb.2013.09.002.
  • 40.
    Golshani Y, Mohammadi S, Golshani M. [Effects of Rhus Coriaria essential oil on depression and anxiety in male rats]. Feyz Med Sci J. 2019;23(5):476-84. Persian.
  • 41.
    Chen CH, Liu TZ, Chen CH, Wong CH, Chen CH, Lu FJ, et al. The efficacy of protective effects of tannic acid, gallic acid, ellagic acid, and propyl gallate against hydrogen peroxide-induced oxidative stress and DNA damages in IMR-90 cells. Mol Nutr Food Res. 2007;51(8):962-8. [PubMed ID:17628875]. https://doi.org/10.1002/mnfr.200600230.

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© 2025, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.

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