TiO2 Nanoparticle as a Sensitizer Drug in Radiotherapy: in Vitro Study

Mostafa Rezaei-Tavirani; Elham Dolat; Hadi Hasanzadeh; Samaneh-Sadat Seyyedi; Vahid Semnani; Sara Sobhi

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

Outlines

Abstract
Copyright

TiO2 Nanoparticle as a Sensitizer Drug in Radiotherapy: in Vitro Study

Author(s):

Mostafa Rezaei-Tavirani¹,

Elham Dolat²,

Hadi Hasanzadeh^3,*,

Samaneh-Sadat Seyyedi⁴,

Vahid Semnani⁵,

Sara Sobhi⁶

1Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2Dept. of Radiology, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3Dept. of Medical Physics, Semnan University of Medical Sciences, Semnan, Iran

4Faculty of medicine, Tehran University of Medical Sciences, Tehran, Iran

5Dept. of Pathology, Semnan University of Medical Sciences, Semnan, Iran

6Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

International Journal of Cancer Management:Vol. 6, issue Supplement; e80460

Published online:Dec 30, 2013

Article type:Research Article

Received:Jan 02, 2013

Accepted:Feb 20, 2013

How to Cite:Mostafa Rezaei-TaviraniElham DolatHadi HasanzadehSamaneh-Sadat SeyyediVahid SemnaniSara Sobhiet al.TiO2 Nanoparticle as a Sensitizer Drug in Radiotherapy: in Vitro Study.Int J Cancer Manag.6(Supplement):e80460.

Abstract

Background: Radiosensitizer drugs are used to enhance the efficiency of radiotherapy. Some nanoparticles can be considered as radiosensitizers, because they enhance cytotoxicity due to oxidative stress and increase free radical yield, especially ROS, within cells resulting to cell death.

Methods: In this study, synergistic effect of TiO2 nanoparticles was evaluated in presence of 60Co gamma rays on human breast cancer (MCF-7) and gastric cancer (MKN-45) cell lines. After cell culture, cells were exposed to several doses of gamma rays and a dose of 2Gy was selected due to survival analysis. Next, several doses of nanoparticle from each type was applied and cell survival was analyzed from which a dose of 30µg/ml was selected for the remainder of study. Finally, synergistic effect of gamma rays and nanoparticles was evaluated in two time delay groups using MTT assay.

Results: Viability of cells in presence of gamma radiation and nanoparticles, significantly reduced compared to viability of cells exposed only to radiation or nanoparticle, alone (P-value≤0.05). The effect was dependent on nanoparticle type, time between addition of nanoparticle to cells and exposure to gamma rays and also cell dependent.

Conclusion: TiO2 increased sensitivity of cancer cells to gamma radiation, due to an increase in ROS production and cytotoxicity. Anatase crystals have more severe effects than Rutile crystal because of having a larger surface area and creation of more free radicals. Therefore, this nanoparticle has the potential to be used as a radiosensitizer and further studies should be considered on other cell lines and in vivo.

Keywords

Fulltext

The Full text is available in PDF.

comments