Lead exposure impairs the NMDA agonist-induced NOS expression in pyramidal hippocampal cells

Seyed Nasser Ostad; Mohammad Hossain Ghahremani

doi:10.22037/ijpr.2010.687

IJ Pharmaceutical Research

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https://doi.org/10.22037/ijpr.2010.687

Lead exposure impairs the NMDA agonist-induced NOS expression in pyramidal hippocampal cells

Author(s):

Seyed Nasser Ostad^1,*,

Mohammad Hossain Ghahremani¹

1Cellular and Molecular Research Laboratory, Department of Toxicology and Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences Research Center, Medical Sciences, University of Tehran, Iran

IJ Pharmaceutical Research:Vol. 5, issue 4; 261-267

Published online:Nov 20, 2010

Article type:Research Article

Received:Nov 21, 2005

Accepted:Jul 21, 2006

How to Cite:Seyed Nasser OstadMohammad Hossain GhahremaniLead exposure impairs the NMDA agonist-induced NOS expression in pyramidal hippocampal cells.Iran J Pharm Res.5(4):e128297.https://doi.org/10.22037/ijpr.2010.687.

Abstract

Chronic exposure to lead (Pb) affects neural functions in central nervous system (CNS) particularly the learning and memory. On the other hand, alteration of calcium level in the CNS results in activation of NOS. It has been shown that lead enters the neurons through calcium channels and displaces Ca²⁺ from calcium binding proteins such as calmodulin and troponin C thereby affecting calcium-mediated processes.

Our recently data showed that no prodaction due to NMDA receptor simulation in cultured CA1 pyramidal cells has been diminished in the presence of 10 nM of Lead acetate. Therefore, it is possible that Lead can inhibit the elevation of NO through blockade of NMDA receptor and interference of LTP through this mechanism. This finding may attribute to the effect of lead on the NOS activity or expression as key enzyme producing NO. In this study we have examined the effect of lead acetate on the NOS expression in the presence of NMDA agonist using immunocytochemical analysis. Expression of nNOS were examined in the CA1 pyramidal cells exposed to 10 and 100 nM lead acetate and 40 µM ACBD (NMDA agonist). The result of this experiment showed that the enhanced nNOS expression induced by ACBD significantly diminished by lead acetate. The trend of this inhibition is similar to amount of NO production indicating that the decrease of expression may major reason of decrease in NO production.

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Author(s):

Seyed Nasser Ostad:[PubMed][Scholar]
Mohammad Hossain Ghahremani:[PubMed][Scholar]