Prevalence of Extended-Spectrum Beta-lactamases (ESBLs) in  Pseudomonas aeruginosa Isolated from Hospitalized Patients in  Imam Reza and Imam Khomeini hospitals in Kermanshah, 2013‎

Fardin  Zoghi Maleki‎; Mohammad Reza  Nahaei‎; Nasrollah  Sohrabi‎; Laya   Rezavand‎

Journal of Clinical Research in Paramedical Sciences

The Official Journal of Paramedical School, KUMS

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Prevalence of Extended-Spectrum Beta-lactamases (ESBLs) in Pseudomonas aeruginosa Isolated from Hospitalized Patients in Imam Reza and Imam Khomeini hospitals in Kermanshah, 2013‎

Author(s):

Fardin Zoghi Maleki‎¹,

Mohammad Reza Nahaei‎^2,*,

Nasrollah Sohrabi‎³,

Laya Rezavand‎⁴

1Faculty of Basic Sciences,‎‏ ‏Ahar Islamic of Azad‏ ‏University, Ahar‎, Iran

2Department of‏ ‏Microbiology, School of‏ ‏Medicine, Tabriz University‏ ‏of Medical Sciences, ‎Tabriz‎, Iran

3Department of Medical‏ ‏Laboratory Sciences, School‏ ‏of Paramedicine,‎‏ ‏Kermanshah ‎University of‏ ‏Medical Sciences,‎‏ ‏Kermanshah, Iran

4Kermanshah University of‏ ‏Medical Sciences,‎‏ ‏Kermanshah‎, Iran

Journal of Clinical Research in Paramedical Sciences:Vol. 4, issue 3; e82033

Published online:Oct 10, 2015

Article type:Research Article

Received:Jan 05, 2015

Accepted:Aug 13, 2015

How to Cite:Fardin Zoghi Maleki‎Mohammad Reza Nahaei‎Nasrollah Sohrabi‎Laya Rezavand‎Prevalence of Extended-Spectrum Beta-lactamases (ESBLs) in Pseudomonas aeruginosa Isolated from Hospitalized Patients in Imam Reza and Imam Khomeini hospitals in Kermanshah, 2013‎.J Clin Res Paramed Sci.4(3):e82033.

Abstract

Background: Pseudomonas aeruginosa is one of the most common Gram negative bacteria involved in nosocomial infections. Presence of Extended-spectrum Beta-lactamases (ESBLs) genes, play an important role in induction of resistance in beta-lactamase producing species to beta lactam antibiotics. Frequency of resistance to these antibiotics is increasing. In this study, antibiotic sensitivity and frequency of ESBLs in Pseudomonas aeruginosa which had been isolated from clinical specimens, was assessed by phenotypic
methods.
Methods: This study was performed on 200 isolated Pseudomonas aeruginosa from clinical specimens in Imam Khomeini and Imam Reza hospitals, Kermanshah during 2013. Specimens were determined and cultured by standard conventional methods. Antibiotic sensitivity test on 14 antibacterial agents was performed according to CLSI criteria.
Then, frequency of ESBLs producing isolates was determined by Combined Disk Test (CDT) and Double Disk Synergy Test (DDST) methods.
Results: This study revealed that the highest and lowest resistance rate were against to cefpodoxime (100%) and piperacilin-tazobactam (34%), respectively. Using CDT and DDST methods, 41% of isolates were ESBL positive.
Conclusion: Considering high resistance rate of Pseudomonas aeruginosa to majority of antibiotics and increasing frequency of ESBLs producing isolates, we need for infection control criteria and use of appropriate therapeutic protocols according to antibiogram
test patterns.

Keywords

Pseudomonas aeruginosa

Extended-Spectrum Betalactamases

Antibiotic resistance

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