Keywords
Dear Editor,
Nowadays, essential oils are used in different products such as food as natural antioxidants, antibacterial and flavoring compounds (1). Essential oils are used as a replacement for chemical preservatives such as butylated hydroxytoluene (BHT) and tertiary butyl hydroquinone (TBHQ) that can threaten human health as cancer promoters (2). Nearly 3000 different essential oils are known today, and 300 are available and used commercially (3). They are obtained from aromatic plants and their various parts including roots, stems, leaves, flowers, fruits and seeds (4). The aim of this study was to evaluate the antioxidant activity and antibacterial effects of Apium petroselinum and Portulaca oleracea essential oils on food spoilage bacteria. A. petroselinum and P. oleracea were purchased from the local grocery stores of Shahrekord city and authenticated by standard botanic work at the Medical Plants Research Center of Shahrekord University of Medical Sciences, Iran. Essential oils were then extracted by steam distillation for three hours using a Clevenger-type apparatus and dried by adding anhydrous sodium sulfate and stored at 4°C, before being used for the assay. Total phenolic contents in the obtained essence were examined by the Folin Ciocalteu method and the results were expressed as milligrams of gallic acid equivalents (GAEs)/gram of essential oil. The total antioxidant capacities of their essential oils were assayed using β-carotene and linoleic acid (5).
Cultures of Alcaligenes faecalis (PTCC 1624), Providencia rettgeri (PTCC 1512), Serratia marcescens (PTCC 1621), Klebsiella oxytoca (PTCC 1402), Staphylococcus aureus (PTCC 1169), Shigella dysenteriae (PTCC 1188) and Listeria monocytogenes (PTCC 1163) were purchased from the Iranian Research Organization for Science and Technology (IROST). The micro-dilution method in sterile 96-microwell plates was used to obtain the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) (6). Each experiment was done in triplicates. Based on the obtained results, the two essential oils demonstrated antioxidant and antibacterial activities. The most sensitive bacteria were A. faecalis and S. dysenteriae, which are Gram-negative bacteria (Table 1). The results obtained in this work are in agreement with pervious studies. Earlier studies have reported that various bacteria such as S. dysenteriae, E. coli, Helicobacter pylori and S. aureus were sensitive to essential oils and extracts of the above mentioned plants (7, 8). However, the Gram-negative bacteria were more resistant than Gram-positive species, due to restricted diffusion of the hydrophobic compounds by the hydrophilic cell wall structure containing lipo-polysaccharide (LPS) (9). In conclusion, the study results suggest that replacement of synthetic preservatives with natural additives as a good source of healthy compounds, namely phenols (10), can be useful in the food industry to increase shelf life of food products.
Antioxidant and Antibacterial Characteristics of Apium petroselinum and Portulaca oleracea Essential Oils a
| Apium petroselinum | Portulaca oleracea | BHTa | |
|---|---|---|---|
| Total Phenolic Content (mg GAEs/g) | 8.18 ± 0.45 | 7.8 ± 0.2 | - |
| Antioxidant Capacity, % | 45 ± 1.52 | 56.5 ± 4.19 | 90.6 ± 3.30 |
| Antibacterial activity | |||
| Alcaligenes faecalis | |||
| MIC mg/mL | 1.562 | 6.25 | - |
| MBC, mg/mL | 3.125 | 12.5 | - |
| Providencia rettgeri | |||
| MIC, mg/mL | 6.25 | 12.5 | - |
| MBC, mg/mL | 12.5 | 25 | - |
| Serratia marcescens | |||
| MIC mg/mL | 3.125 | 12.5 | - |
| MBC, mg/mL | 6.25 | 25 | - |
| Klebsiella oxytoca | |||
| MIC, mg/mL | 12.5 | 12.5 | - |
| MBC, mg/mL | 25 | 25 | - |
| Staphylococcus aureus | |||
| MIC, mg/mL | 12.5 | 12.5 | - |
| MBC, mg/mL | 25 | 25 | - |
| Shigella dysenteriae | |||
| MIC, mg/mL | 1.562 | 6.25 | - |
| MBC, mg/mL | 3.125 | 12.5 | - |
| Listeria monocytogenes | |||
| MIC, mg/mL | 12.5 | 6.25 | - |
| MBC, mg/mL | 25 | 12.5 | - |
Acknowledgements
References
-
1.
Shaaban HAE, El-Ghorab AH, Shibamoto T. Bioactivity of essential oils and their volatile aroma components: Review. J Essent Oil Res. 2012;24(2):203-12. https://doi.org/10.1080/10412905.2012.659528.
-
2.
Hyldgaard M, Mygind T, Meyer RL. Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Front Microbiol. 2012;3:12. [PubMed ID: 22291693]. https://doi.org/10.3389/fmicb.2012.00012.
-
3.
Burt S. Essential oils: their antibacterial properties and potential applications in foods--a review. Int J Food Microbiol. 2004;94(3):223-53. [PubMed ID: 15246235]. https://doi.org/10.1016/j.ijfoodmicro.2004.03.022.
-
4.
Sanchez E, Garcia S, Heredia N. Extracts of edible and medicinal plants damage membranes of Vibrio cholerae. Appl Environ Microbiol. 2010;76(20):6888-94. [PubMed ID: 20802077]. https://doi.org/10.1128/AEM.03052-09.
-
5.
Sharafati Chaleshtori R, Sharafati Chaleshtori F, Rafieian Kopae M. Biological characterization of Iranian walnut (Juglans regia) leaves. Turk J Biol. 2011;35:635-9.
-
6.
Sharafati Chaleshtori R, Rokni N, Razavilar V, Rafieian Kopaei M. The Evaluation of the Antibacterial and Antioxidant Activity of Tarragon (Artemisia dracunculus L.) Essential Oil and Its Chemical Composition. Jundishapur J Microbiol. 2013;6(9). https://doi.org/10.5812/jjm.7877.
-
7.
Nakhaei Moghaddam M. In vitro anti-bacterial activity of methanolic extract of Apium petroselinum L. seed against clinical isolates of Helicobacter pylori. Daneshvar Med. 2010;17:63-70.
-
8.
Bae JH. Antimicrobial Effect of Portulaca oleracea Extracts on Food-Borne Pathogens. J Food Sci Nutr. 2004;9(4):306-11. https://doi.org/10.3746/jfn.2004.9.4.306.
-
9.
Sulaiman S, Ibrahim D, Kassim J, Sheh-Hong L. Antimicrobial and antioxidant activities of condensed tannin from Rhizophora apiculata barks. J Chem Pharm Res. 2011;3(4):436-44.
-
10.
Rafieian-Kopaei M, Baradaran A, Rafieian M. Plants antioxidants: From laboratory to clinic. J Nephropathol. 2013;2(2):152-3. [PubMed ID: 24475444]. https://doi.org/10.12860/JNP.2013.26.