Quantitative and Qualitative Analyses of the Constituents of the Hydroalcoholic Extract of Quercus infectoria Gall from Kermanshah and Evaluation of Its Antioxidant and Antibacterial Activities

Negar Mohammadzadeh; Masoud Ghiasian; Javad Faradmal; Dara Dastan

doi:10.4103/jrptps.JRPTPS_36_21

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https://doi.org/10.4103/jrptps.JRPTPS_36_21

Quantitative and Qualitative Analyses of the Constituents of the Hydroalcoholic Extract of Quercus infectoria Gall from Kermanshah and Evaluation of Its Antioxidant and Antibacterial Activities

Author(s):

Negar Mohammadzadeh¹,

Masoud Ghiasian²,

Javad Faradmal³,

Dara Dastan^1,*

1Department of Pharmacognosy, School of Pharmacy, Medicinal Plants and Natural Products Research Center, Hamadan, Iran

2Department of Neurology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

3Department of Biostatistics and Epidemiology, School of Public Health, Hamadan University of Medical Sciences, Hamadan, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 10, issue 2; 287-93

Published online:Dec 17, 2021

Article type:Research Article

Received:Mar 15, 2021

Accepted:Aug 14, 2021

How to Cite:Negar MohammadzadehMasoud GhiasianJavad FaradmalDara DastanQuantitative and Qualitative Analyses of the Constituents of the Hydroalcoholic Extract of Quercus infectoria Gall from Kermanshah and Evaluation of Its Antioxidant and Antibacterial Activities.J Rep Pharm Sci.10(2):e146968.https://doi.org/10.4103/jrptps.JRPTPS_36_21.

Abstract

Background and Purpose: Quercus infectoria is a species of Quercus genus (Fagaceae) whose galls are known in traditional medicine for their antibacterial, analgesic, anti-inflammatory, and astringent effects. The present study aimed to carry out quantitative and qualitative analyses of the constituents of the hydroalcoholic extract of the Q. infectoria galls from Kermanshah and to evaluate its antioxidant and antibacterial activities.
Materials and Methods: Following the extraction process using ethanol/water (70/30), phytochemical tests were done. Total phenol and flavonoid and antioxidant and antibacterial activities against specific strains of bacteria were evaluated. Some of the constituents of the extract were identified using high-performance liquid chromatography-photodiode array, and their amount was obtained.
Results: The phytochemical tests proved that the extract contained alkaloid, flavonoid, tannin, saponin, and phenolic compounds. The amount of total phenolic and flavonoid compounds was 16.21 and 1.78 mg/g dried galls, respectively. The IC50 value of the antioxidant constituents of the extract was 47 μg/ mL. The results of the antimicrobial assay showed the high activity of the extract against Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, and S. epidermidis. The quantitative analysis of the extract confirmed the presence of gallic acid, rutin, quercetin, benzoic acid, and caffeic acid (12.30, 10.72, 5.00, 9.25, and 3.94 mg/g dried galls, respectively).
Conclusion: Considering the results of this study, the extract of Q. infectoria galls could be used as a primary substance in treating bacterial infections and oxidative stress-related diseases.

Keywords

phytochemical screening

Quercus infectoria

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Author(s):

Negar Mohammadzadeh:[PubMed][Scholar]
Masoud Ghiasian:[PubMed][Scholar]
Javad Faradmal:[PubMed][Scholar]
Dara Dastan:[PubMed][Scholar]