Simultaneous RP-HPLC Estimation and Validation of Metronidazole, Furazolidone, and Dicyclomine in Capsule

Amuthalakshmi Sivaperuman; Nalini Calambur Nagarajan; Uma Jayapaul

doi:10.4103/jrptps.JRPTPS_104_19

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https://doi.org/10.4103/jrptps.JRPTPS_104_19

Simultaneous RP-HPLC Estimation and Validation of Metronidazole, Furazolidone, and Dicyclomine in Capsule

Author(s):

Amuthalakshmi Sivaperuman^1,*,

Nalini Calambur Nagarajan¹,

Uma Jayapaul²

1Department of Pharmaceutical Analysis, C. L. Baid Metha College of Pharmacy, Thorapakkam, India

2Twenty First Century Pharmaceuticals Private Limited, Ambattur, Chennai, Tamil Nadu, India

Journal of Reports in Pharmaceutical Sciences:Vol. 10, issue 1; 15-21

Published online:May 31, 2021

Article type:Research Article

Received:Oct 30, 2019

Accepted:Mar 02, 2021

How to Cite:Amuthalakshmi SivaperumanNalini Calambur NagarajanUma JayapaulSimultaneous RP-HPLC Estimation and Validation of Metronidazole, Furazolidone, and Dicyclomine in Capsule.J Rep Pharm Sci.10(1):e146975.https://doi.org/10.4103/jrptps.JRPTPS_104_19.

Abstract

For the first time, a new, simple, precise reversed-phase high-performance liquid chromatography method was developed for the simultaneous estimation of metronidazole, furazolidone, and dicyclomine hydrochloride in capsule dosage form. The method was performed with Thermo, C8 (150 mm×4.6) column. The best separation was achieved by gradient elution with mobile phase of acetonitrile, water (40:60), and 20 mm phosphate buffer with 10% w/v sodium hydroxide (pH 7.5) with a detection wavelength of 215 nm. The separation was completed within 15 min of runtime. The retention time of metronidazole, furazolidone, and dicyclomine hydrochloride was found to be 1.79, 2.45, and 11.50 min, respectively. The proposed method was found to be linear. The method was statistically validated as per the ICH guidelines and shown to be simple, accurate, precise, linear, and reproducible in the range of 40.2–60, 40.2–60.4, and 3–5 μg/mL for metronidazole, furazolidone, and dicyclomine, respectively. For the first time, the developed method foretells the suitability of the method for the simultaneous estimation of three drugs in the commercially available dosage forms.

Keywords

Dicyclomine hydrochloride

furazolidone

metronidazole

RP-HPLC

simultaneous estimation

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Author(s):

Amuthalakshmi Sivaperuman:[PubMed][Scholar]
Nalini Calambur Nagarajan:[PubMed][Scholar]
Uma Jayapaul:[PubMed][Scholar]