The Wound Healing Effect of Allogeneic Freeze-Dried Platelet-Rich Plasma in a Full-Thickness Wound Animal Model

Iswinarno Saputro; Andi Muhammad Ardan; Wiwik M Yuniarti; Oki Nugraha Putra

doi:10.4103/jrptps.JRPTPS_77_19

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https://doi.org/10.4103/jrptps.JRPTPS_77_19

The Wound Healing Effect of Allogeneic Freeze-Dried Platelet-Rich Plasma in a Full-Thickness Wound Animal Model

Author(s):

Iswinarno Saputro¹,

Andi Muhammad Ardan¹,

Wiwik M Yuniarti²,

Oki Nugraha Putra^3,*

1Faculty of Medicine, Airlangga University - General Hospital of Dr. Soetomo, Surabaya, Indonesia

2Department of Veterinary Clinic, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia

3Study Program of Pharmacy, Faculty of Medicine, Hang Tuah University, Surabaya, Indonesia

Journal of Reports in Pharmaceutical Sciences:Vol. 10, issue 1; 71-6

Published online:May 31, 2021

Article type:Research Article

Received:Jul 24, 2019

Accepted:Aug 05, 2020

How to Cite:Iswinarno SaputroAndi Muhammad ArdanWiwik M YuniartiOki Nugraha PutraThe Wound Healing Effect of Allogeneic Freeze-Dried Platelet-Rich Plasma in a Full-Thickness Wound Animal Model.J Rep Pharm Sci.10(1):e147092.https://doi.org/10.4103/jrptps.JRPTPS_77_19.

Abstract

Background: Epithelialization is an indicator of wound healing. Platelet-rich plasma (PRP) may accelerate the epithelialization of the wound due to high amount of growth factors. Generally, allogeneic PRP provides a smaller immunological effect than autologous PRP. By a freeze-drying method, it is assumed that allogeneic PRP has lower allergenic activity.
Aims and Objectives: The aim of this study was to investigate the effect of allogeneic freeze-dried PRP on wound healing of a full-thickness wound in New Zealand rabbits. About 2 × 2 cm2 full-thickness wounds were created on rabbits using a template on both sides of the dorsum and divided into treatment group and control group. The treatment group was treated with allogeneic freeze-dried PRP and the control group was treated with moist dressing. Acceleration of wound healing was shown by the epithelialization and measured on day 7 using digital Visitrak. Nine New Zealand rabbits were used in this study.
Results: In the treatment group, the epithelialization was significantly higher 3.00 ± 0.96 cm2 than the control group 1.35 ± 0.85 cm2 (P < 0.000) by independent t test. Another finding of our study was the allergic reaction was not observed after the administration of allogeneic freeze-dried PRP in rabbits.
Conclusion: Our results indicate that allogeneic freeze-dried PRP accelerates epithelialization compared to the control group and does not cause an allergic reaction in full-thickness wounds in rabbits. The effect of allogeneic PRP, prepared with a freeze-drying method, on the process of wound healing is reported for the first time in this article.

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Author(s):

Iswinarno Saputro:[PubMed][Scholar]
Andi Muhammad Ardan:[PubMed][Scholar]
Wiwik M Yuniarti:[PubMed][Scholar]
Oki Nugraha Putra:[PubMed][Scholar]