Apoptotic Effects of Ginger Extract (Zingiber officinale) on Esophageal Cancer Cells ESO26: An In Vitro Study

Abbas Abbasi; Ali Azizi; Seyed Mostafa Nachvak; Elaheh Alizadeh; Rezvan Abbsavaran; Elham Mirtaheri; Mehdi Moradinazar; Mehrali Rahimi

doi:10.4103/jrptps.JRPTPS_98_19

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https://doi.org/10.4103/jrptps.JRPTPS_98_19

Apoptotic Effects of Ginger Extract (Zingiber officinale) on Esophageal Cancer Cells ESO26: An In Vitro Study

Author(s):

Abbas Abbasi¹,

Ali Azizi

²,

Seyed Mostafa Nachvak¹,

Elaheh Alizadeh¹,

Rezvan Abbsavaran¹,

Elham Mirtaheri¹,

Mehdi Moradinazar³,

Mehrali Rahimi^4,*

1Faculty of Nutrition and Food Industry, Pharmaceutical Science Research Center, Kermanshah, Iran

2Department of Community Medicine, Faculty of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

3Assistant Professor in Epidemiology, Behavioral Disease Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

4Diabetes Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 9, issue 2; 183-8

Published online:Oct 07, 2020

Article type:Research Article

Received:Sep 14, 2019

Accepted:May 10, 2020

How to Cite:Abbas AbbasiAli AziziSeyed Mostafa NachvakElaheh AlizadehRezvan AbbsavaranElham MirtaheriMehdi MoradinazarMehrali Rahimiet al.Apoptotic Effects of Ginger Extract (Zingiber officinale) on Esophageal Cancer Cells ESO26: An In Vitro Study.J Rep Pharm Sci.9(2):e147111.https://doi.org/10.4103/jrptps.JRPTPS_98_19.

Abstract

Aim: Ginger is a natural dietary rhizome with antioxidant, anti-inflammatory, and anticarcinogenic properties. It has many medical beneficial properties such as anti-proliferation and antiapoptotic effects on cancerous esophageal cells.
Materials and Methods: Esophageal cancer cells ESO26 were cultured in the presence and absence of ginger extract at various concentrations for 12, 18, and 24 h. Then, the viability was determined by 3-(4,5-dimethylthiazol)-2,5-diphenyl tetrazolium bromide (MTT) assay. Western blot analysis of caspase-3 was performed to detect apoptosis. p21, Bax, and Bcl-2 gene expression was measured using quantitative polymerase chain reaction (PCR). Data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey test.
Results: The ginger extract increased the cleavage of caspase-3 in cells (P < 0.05). Results of real-time PCR have shown that ginger decreased the expression of Bcl-2 and increased Bax and p21 gene expression (P < 0.05).
Conclusions: Results showed that the process of cell proliferation has been stopped. Also, this study indicated that ginger might exert a chemopreventive effect on esophageal cancer through the suppression of proliferation and the growth of tumor cells as well as the induction of apoptosis.

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Author(s):

Abbas Abbasi:[PubMed][Scholar]
Ali Azizi:[PubMed][Scholar]
Seyed Mostafa Nachvak:[PubMed][Scholar]
Elaheh Alizadeh:[PubMed][Scholar]
Rezvan Abbsavaran:[PubMed][Scholar]
Elham Mirtaheri:[PubMed][Scholar]
Mehdi Moradinazar:[PubMed][Scholar]
Mehrali Rahimi:[PubMed][Scholar]