Fosfomycin Protects Intestinal Cells from Nuclear Changes Suggestive of Deoxynivalenol-induced Apoptosis

Denisa Pérez Gaudio; Joaquín Mozo; Guadalupe Martínez; María B. Fernández Paggi; Julieta M. Decundo; Agustina Romanelli; Susana Dieguez; Alejandro Soraci

doi:10.4103/jrptps.JRPTPS_124_19

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https://doi.org/10.4103/jrptps.JRPTPS_124_19

Fosfomycin Protects Intestinal Cells from Nuclear Changes Suggestive of Deoxynivalenol-induced Apoptosis

Author(s):

Denisa Pérez Gaudio^{1, 2,*},

Joaquín Mozo³,

Guadalupe Martínez^{1, 2},

María B. Fernández Paggi^{1, 2},

Julieta M. Decundo^{1, 2},

Agustina Romanelli^{1, 2},

Susana Dieguez^{1, 4},

Alejandro Soraci^{1, 2}

1Área de Toxicología, Depto. de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Tandil, Argentina

2Centro de Investigación Veterinaria de Tandil (CIVETAN-CONICET), Tandil, Argentina

3Agencia Nacional de Promoción Científica y Tecnológica (ANPCyT), Ciudad Autónoma de Buenos Aires, Tandil, Argentina

4Comisión de Investigaciones Científicas de la Provincia de Buenos Aires, La Plata, Argentina

Journal of Reports in Pharmaceutical Sciences:Vol. 9, issue 2; 209-14

Published online:Oct 07, 2020

Article type:Research Article

Received:Nov 15, 2019

Accepted:May 14, 2020

How to Cite:Denisa Pérez GaudioJoaquín MozoGuadalupe MartínezMaría B. Fernández PaggiJulieta M. DecundoAgustina RomanelliSusana DieguezAlejandro Soraciet al.Fosfomycin Protects Intestinal Cells from Nuclear Changes Suggestive of Deoxynivalenol-induced Apoptosis.J Rep Pharm Sci.9(2):e147115.https://doi.org/10.4103/jrptps.JRPTPS_124_19.

Abstract

Background: Fosfomycin (FOS) is a broad-spectrum antibiotic that inhibits cell wall synthesis. It has bactericidal activity against both gram-positive and gram-negative bacteria. FOS also promotes phagocytosis, has immunomodulatory effects, and protects against the toxicity caused by other drugs. On the contrary, deoxynivalenol (DON) causes cytotoxicity on tissues of rapid growth and fast turnover.
Objectives: The aim of this study was to determine the percentage of nuclear changes indicative of DONinduced apoptosis on intestinal cell cultures (Caco-2) and to evaluate the protective effect of FOS on mycotoxin-exposed cells.
Materials and Methods: Cell cultures were treated as follows: (1) DON: 2.8 μg/mL, (2) calcium FOS: 580 μg/mL, (3) DON 2.8 μg/mL + calcium FOS 580 μg/mL, and (4) negative control. Nuclear morphology was evaluated in fixed cells stained with 4′,6-diamino-2-phenylindol and then visualized under an immunofluorescence microscope.
Results: Percentages of cells with nuclear changes were significantly higher in cells treated with DON (31.53% } 4.17%) compared to those incubated with the antibiotic in conjunction with the mycotoxin (5.63% } 4.23%). On the contrary, there were no significant differences between cells incubated with DON + FOS and cells incubated only with the antibiotic (1.10% } 1.55%) when compared to the negative control (3.50% } 0.09%).
Conclusion: The results from this study showed that DON induces nuclear changes suggestive of apoptosis in intestinal cells and that FOS can protect cells from DNA damage. Further studies are needed to determine whether DON induces apoptosis only on cells of epithelial origin and to understand the implications of FOS protective effect under in vivo conditions.

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Author(s):

Denisa Pérez Gaudio:[PubMed][Scholar]
Joaquín Mozo:[PubMed][Scholar]
Guadalupe Martínez:[PubMed][Scholar]
María B. Fernández Paggi:[PubMed][Scholar]
Julieta M. Decundo:[PubMed][Scholar]
Agustina Romanelli:[PubMed][Scholar]
Susana Dieguez:[PubMed][Scholar]
Alejandro Soraci:[PubMed][Scholar]