In Vitro Cytotoxic Activity of Verbascum alceoides against Cervix Carcinoma Cells

Masoud Sadeghi Dinani; Samin Malakooti; Vajihe Akbari

doi:10.4103/jrptps.JRPTPS_65_19

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Abstract
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https://doi.org/10.4103/jrptps.JRPTPS_65_19

In Vitro Cytotoxic Activity of Verbascum alceoides against Cervix Carcinoma Cells

Author(s):

Masoud Sadeghi Dinani¹,

Samin Malakooti¹,

Vajihe Akbari^2,*

1Department of Pharmacognosy, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

2Department of Pharmaceutical Biotechnology, and Isfahan Pharmaceutical Research Center, Faculty of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 9, issue 1; 19-24

Published online:Jun 26, 2020

Article type:Research Article

Received:Jun 19, 2019

Accepted:Feb 26, 2020

How to Cite:Masoud Sadeghi DinaniSamin MalakootiVajihe AkbariIn Vitro Cytotoxic Activity of Verbascum alceoides against Cervix Carcinoma Cells.J Rep Pharm Sci.9(1):e147276.https://doi.org/10.4103/jrptps.JRPTPS_65_19.

Abstract

Background: Verbascum species showed various pharmacological activities including anti-inflammatory, antitussive, antiulcerogenic, immunomodulatory, antimicrobial, antimalarial, antioxidant, and anticancer activities.
Objectives: The aim of this work was to evaluate cytotoxicity of different fractions of Verbascum alceoides, which belongs to this genus.
Materials and Methods: Aerial parts of this plant were collected from Doveiseh area in Kordestan province. The plant was extracted using a four-step extraction method with increasing solvent polarity (i.e., hexane, dichloromethane, chloroform-methanol [9:1], and methanol). The methanol extract was finally separated between water and butanol. Hexane, dichloromethane, chloroformmethanol, butanol, and aqueous partitions were then subjected to cytotoxicity evaluation. Showing the most potent cytotoxic effects, the butanolic partition was further fractionated by medium-performance liquid chromatography and similar eluates were pooled to prepare five final butanolic fractions, named A–E.
Results: In vitro cytotoxicity of these fractions against human cervical epithelioid carcinoma (HeLa) and human umbilical vein endothelial cell (HUVEC) was evaluated using 3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide assay. Fractions D, E, and A showed a significant and dose-dependent inhibition of cell proliferation (half maximal inhibitory concentration [IC50] of 30, 39.8, and 188.6 μg/mL, respectively). According to the preliminary thin-layer chromatography analysis, these cytotoxic effects may be mainly due to presence of saponin and flavonoid compounds.
Conclusion: Future studies will be aimed to isolate and purify active constituents and investigate the effect of them on more different kinds of cancer cells.

Keywords

Cytotoxic effect

human cervical epithelioid carcinoma (HeLa)

human umbilical vein endothelial cell (HUVEC)

Verbascum alceoides

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Author(s):

Masoud Sadeghi Dinani:[PubMed][Scholar]
Samin Malakooti:[PubMed][Scholar]
Vajihe Akbari:[PubMed][Scholar]