Improvement of Antiproliferative Activity of Recombinant Truncated Form of Pseudomonas aeruginosa Exotoxin (PE38) by Vitamin E in MCF-7 Cells

Vahid Asgary; Razieh Bigdeli; Fahimeh Baghbani-Arani; Atieh Hashemi

doi:10.4103/jrptps.JRPTPS_114_19

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https://doi.org/10.4103/jrptps.JRPTPS_114_19

Improvement of Antiproliferative Activity of Recombinant Truncated Form of Pseudomonas aeruginosa Exotoxin (PE38) by Vitamin E in MCF-7 Cells

Author(s):

Vahid Asgary¹,

Razieh Bigdeli¹,

Fahimeh Baghbani-Arani²,

Atieh Hashemi

^3,*

1Research and Development Laboratory, Javid Biotechnology Institute, Tehran, Iran

2Department of Genetics and Biotechnology, School of Biological Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran

3Department of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 9, issue 1; 41-5

Published online:Jun 26, 2020

Article type:Research Article

Received:Oct 13, 2019

Accepted:Mar 02, 2020

How to Cite:Vahid AsgaryRazieh BigdeliFahimeh Baghbani-AraniAtieh HashemiImprovement of Antiproliferative Activity of Recombinant Truncated Form of Pseudomonas aeruginosa Exotoxin (PE38) by Vitamin E in MCF-7 Cells.J Rep Pharm Sci.9(1):e147279.https://doi.org/10.4103/jrptps.JRPTPS_114_19.

Abstract

Background: In addition to beneficial roles of vitamin E in many metabolic processes and its antitumor activities, vitamin E derivatives have extensively been considered as permeability enhancers. Using these enhancers, permeability of a wide spectrum of drugs was reported to be significantly increased. PE38, a toxic substance with a potential application in cancer therapy, is a truncated form of Pseudomonas exotoxin A (PE), which lacks Ia and a portion of domain Ib.
Objective: Here, the antiproliferative potential of PE38 and alpha-tocopherol (αT) form of vitamin E were assessed in MCF-7 cells. The role of vitamin E in PE38 cytotoxicity level was also evaluated.
Materials and Methods: The antiproliferative potential of PE38, vitamin E, and a combination of them were colorimetrically evaluated in a cellular breast cancer model (MCF-7), using the MTT assay. P values of <0.05 were considered significant.
Results: Compared to control cells, the PE38 inhibited the proliferation of MCF-7 cells (80% ± 1.37% cell viability) only at the highest concentration used (500 μg/mL) (P < 0.05). MTT assay also showed that 0.1, 1, and 10mg/mL of vitamin E could significantly (P < 0.001) decrease the cell viability of MCF-7 cells to 57% ± 1.37%, 26.8% ± 1.37%, and 14.7% ± 1.37% at 24 h, respectively. Moreover, the coadministration of vitamin E (0.1 mg/mL) with 31.25, 62.5, 125, 250, and 500 μg/mL concentrations of PE38 decreases in cell viability from 100% in control cells to 35.61% ± 4.29%, 37.8% ± 6.45%, 36.42% ± 5.79%, 32.33% ± 4.62%, and 29.97% ± 5.07% at 24 h, respectively (P < 0.001).
Conclusion: The results of this study suggest that vitamin E can enhance the antiproliferative activity of PE38 toward MCF-7 cells.

Keywords

Antiproliferative activity

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Author(s):

Vahid Asgary:[PubMed][Scholar]
Razieh Bigdeli:[PubMed][Scholar]
Fahimeh Baghbani-Arani:[PubMed][Scholar]
Atieh Hashemi:[PubMed][Scholar]