Angiotensin-converting Enzyme and Renin Inhibition Activities, Antioxidant Properties, Phenolic and Flavonoid Contents of Cuphea ignea A. DC.

Walaa Maged Ismail; Shahira Ezzat; Haidy E. Michel; Kadriya S. El Deeb; Ahlam M. El-Fishawy

doi:10.4103/jrptps.JRPTPS_81_19

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https://doi.org/10.4103/jrptps.JRPTPS_81_19

Angiotensin-converting Enzyme and Renin Inhibition Activities, Antioxidant Properties, Phenolic and Flavonoid Contents of Cuphea ignea A. DC.

Author(s):

Walaa Maged Ismail^1,*,

Shahira Ezzat^{1, 2},

Haidy E. Michel³,

Kadriya S. El Deeb¹,

Ahlam M. El-Fishawy¹

1Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Kasr-El-Ainy Street, Cairo, Egypt

2Department of Pharmacognosy, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), Cairo, Egypt

3Department of Pharmacology and Toxicology, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt

Journal of Reports in Pharmaceutical Sciences:Vol. 9, issue 1; 92-6

Published online:Jun 26, 2020

Article type:Research Article

Received:Aug 02, 2019

Accepted:Oct 10, 2019

How to Cite:Walaa Maged IsmailShahira EzzatHaidy E. MichelKadriya S. El DeebAhlam M. El-FishawyAngiotensin-converting Enzyme and Renin Inhibition Activities, Antioxidant Properties, Phenolic and Flavonoid Contents of Cuphea ignea A. DC..J Rep Pharm Sci.9(1):e147290.https://doi.org/10.4103/jrptps.JRPTPS_81_19.

Abstract

Cuphea ignea A. DC. is an ornamental plant belonging to family Lythraceae distributed in the tropics and temperate zones. Some Cuphea members are traditionally used in treatment of hypertension. The aim of this research was to study the antihypertensive activity of the plant by applying a bio-guided fractionation scheme of the leaves, stems, and flowers using in vitro angiotensin-converting enzyme (ACE) and renin inhibition assays to identify the active organ and its respective active fractions. The aqueous extract and the n-butanol fraction of the leaves showed the highest ACE inhibition activity with IC50 values 0.491 and 0.084 mg/mL, respectively. The renin inhibition activity showed that the alcoholic extract of the leaves had the highest percentage of inhibition (94.82%). Meanwhile, the methylene chloride fraction of the stems showed the highest renin inhibition activity (98.14%) followed by the ethyl acetate fraction of the leaves (93.09%). The antioxidant activity was tested using the 2,2-diphenyl-1-picrylhydrazyl and oxygen radical absorbance activity assays. Correlation studies of the antioxidant activity showed moderate correlation with the phenolic content and strong correlation with the flavonoid content. The renin inhibition activity showed significant positive correlation with the phenolic and flavonoid contents with r values (0.777 and 0.629, respectively).

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Author(s):

Walaa Maged Ismail:[PubMed][Scholar]
Shahira Ezzat:[PubMed][Scholar]
Haidy E. Michel:[PubMed][Scholar]
Kadriya S. El Deeb:[PubMed][Scholar]
Ahlam M. El-Fishawy:[PubMed][Scholar]