Analysis of Phenolics in Calligonum polygonoides In Vitro Cultured Roots

Asmaa I. Owis; Nada S. Abdelwahab; Adel A. Abul-Soad

doi:10.4103/jrptps.JRPTPS_62_18

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https://doi.org/10.4103/jrptps.JRPTPS_62_18

Analysis of Phenolics in Calligonum polygonoides In Vitro Cultured Roots

Author(s):

Asmaa I. Owis^1,*,

Nada S. Abdelwahab²,

Adel A. Abul-Soad³

1Department of Pharmacognosy, Beni-Suef University, Beni-Suef, Egypt

2Department of Pharmacognosy and Analytical Chemistry, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt

3Department of Pharmacognosy and Plant Tissue Culture Laboratory, Horticulture Research Institute, Agricultural Research Center, Giza, Egypt

Journal of Reports in Pharmaceutical Sciences:Vol. 8, issue 2; 124-7

Published online:Dec 31, 2019

Article type:Research Article

How to Cite:Asmaa I. OwisNada S. AbdelwahabAdel A. Abul-SoadAnalysis of Phenolics in Calligonum polygonoides In Vitro Cultured Roots.J Rep Pharm Sci.8(2):e147321.https://doi.org/10.4103/jrptps.JRPTPS_62_18.

Abstract

Background: Calligonum polygonoides L. subsp. Comosum (L’Hér.) Sosk. is an endangered plant species belonging to family Polygonaceae. Although the plant is rich in phytoconstituents and has multipurpose medicinal applications, but in vitro root culture studies and phytochemical investigations of these cultures are rare.
Objectives: To establish in vitro root, callus and cell suspension cultures from in vitro germinated fruits of C. polygonoides to investigate the production of phenolics through root, callus and cell suspension cultures and attempt to enhance cell capacity to accumulate phenolics.
Materials and Methods: Modified Murashige and Skoog medium supplemented with 1 mg l-1 indole-3-butyric acid was used to establish the root culture. Elicitation of cell suspension culture was performed using salicylic acid and yeast extract. The phenolic compounds in root, callus and cell suspension cultures were evaluated using reversed phase high performance liquid chromatography technique.

Results: The unorganized cell suspension culture contained fewer amounts of phenolic compounds than the differentiated roots tissue. Elicitation produced quantitative reprogramming of phenolic content.
Conclusion: The present study provides a chance to improve secondary metabolite yield from this valuable natural plant.

Keywords

Analysis

Calligonum polygonoides

high-performance liquid chromatography

phenolics

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Author(s):

Asmaa I. Owis:[PubMed][Scholar]
Nada S. Abdelwahab:[PubMed][Scholar]
Adel A. Abul-Soad:[PubMed][Scholar]