Acetyl Glycyrrhetinic Acid Methyl Ester as a Promising Glycyrrhizin Derivative against the Breast Cancer Cells (MCF-7)

Mohamed G. Ibrahim; Fardous F. El-Senduny; Magdy M. Youssef; Diaaeldin M. Elimam; Fatma M. Abdel Bar; Farid A. Badria

doi:10.4103/jrptps.JRPTPS_60_18

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https://doi.org/10.4103/jrptps.JRPTPS_60_18

Acetyl Glycyrrhetinic Acid Methyl Ester as a Promising Glycyrrhizin Derivative against the Breast Cancer Cells (MCF-7)

Author(s):

Mohamed G. Ibrahim¹,

Fardous F. El-Senduny¹,

Magdy M. Youssef¹,

Diaaeldin M. Elimam²,

Fatma M. Abdel Bar^{3, 4},

Farid A. Badria^4,*

1Department of Biochemistry, Faculty of Science, Mansoura University, Mansoura, Egypt

2Department of Pharmacognosy, Faculty of Pharmacy, Kafrelsheikh University, Kafr El-sheikh, Egypt

3Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj, Saudi Arabia

4Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura, Egypt

Journal of Reports in Pharmaceutical Sciences:Vol. 8, issue 2; 161-71

Published online:Dec 31, 2019

Article type:Research Article

How to Cite:Mohamed G. IbrahimFardous F. El-SendunyMagdy M. YoussefDiaaeldin M. ElimamFatma M. Abdel BarFarid A. Badriaet al.Acetyl Glycyrrhetinic Acid Methyl Ester as a Promising Glycyrrhizin Derivative against the Breast Cancer Cells (MCF-7).J Rep Pharm Sci.8(2):e147374.https://doi.org/10.4103/jrptps.JRPTPS_60_18.

Abstract

Background: Breast cancer remains the most potent threat to women’s life worldwide. So far, no ideal drug for treatment of breast cancer, all available drugs exhibit severe side effects, poor therapeutic index, and high cost.
Objective: Therefore, this study aimed to investigate the potential use of the natural pentacyclic triterpenoids such as Boswellic, Betulinic (BA), Urosolic, Oleanolic acids, Glycyrrhizin and their derivatives for treatment of breast cancer.
Materials and methods: The cell viability was firstly determined after treatment with 50 μM of each compound. The effect of the treatment on cell cycle, apoptosis, cell migration and colony formation was evaluated. The ability of the new glycyrrhizin derivative to activate p53 was investigated by flow cytometry.
Results: The cytotoxicity assay revealed that glycyrrhizin derivative AM-GA (3-acetyl-18β-glycyrrhetinic-30-methyl ester) and BA were the most cytotoxic against breast cancer cell line MCF-7 with IC50 values 4.5±0.1 and 4±0.1 μM, respectively. Both AM-GA and BA were selective towards breast cancer cells rather than the normal lung fibroblast cell line WI-38. Both AM-GA and BA were able to inhibit the cancer cell migration in the wound healing assay and inhibited colony formation. Studying the mechanism of action revealed that AM-GA inhibited the growth of the breast cancer cells via cell cycle arrest at sub-G1 phase, induction of apoptosis and activation of the tumor suppressor protein p53.
Conclusion: This work highlights the unique role of AM-GA against breast cancer via different mechanisms and will be the gate for new potent analogues and fights different cancer types.

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Author(s):

Mohamed G. Ibrahim:[PubMed][Scholar]
Fardous F. El-Senduny:[PubMed][Scholar]
Magdy M. Youssef:[PubMed][Scholar]
Diaaeldin M. Elimam:[PubMed][Scholar]
Fatma M. Abdel Bar:[PubMed][Scholar]
Farid A. Badria:[PubMed][Scholar]