Comparative Phytochemical Screening, In Vivo Antioxidant and Nephroprotective Effects of Extracts of Cassava Leaves on Paracetamol-intoxicated Rats

Israel O. Okoro; Helen E. Kadiri; Eferhire Aganbi

doi:10.4103/jrptps.JRPTPS_10_19

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https://doi.org/10.4103/jrptps.JRPTPS_10_19

Comparative Phytochemical Screening, In Vivo Antioxidant and Nephroprotective Effects of Extracts of Cassava Leaves on Paracetamol-intoxicated Rats

Author(s):

Israel O. Okoro^1,*,

Helen E. Kadiri¹,

Eferhire Aganbi¹

1Department of Biochemistry, Faculty of Science, Delta State University, Abraka, Delta State, Nigeria

Journal of Reports in Pharmaceutical Sciences:Vol. 8, issue 2; 188-94

Published online:Dec 31, 2019

Article type:Research Article

How to Cite:Israel O. OkoroHelen E. KadiriEferhire AganbiComparative Phytochemical Screening, In Vivo Antioxidant and Nephroprotective Effects of Extracts of Cassava Leaves on Paracetamol-intoxicated Rats.J Rep Pharm Sci.8(2):e147378.https://doi.org/10.4103/jrptps.JRPTPS_10_19.

Abstract

The phytochemical screening, antioxidant, and nephroprotective effects of methanol and acetone extracts of cassava (Manihot esculenta Crantz) leaves were comparatively investigated using standard procedures. Fifty-four male Wistar rats (albino) were divided into nine groups of six rats each. Group 1 = negative control (normal untreated rats + normal saline); group 2 = positive control (rats + 2 g/kg bw acetaminophen + normal saline), groups 3, 4, and 5 = 200 mg/kg bw, 100 mg/kg bw, and 50 mg/kg bw of methanol extract, respectively, + 2 g/kg bw acetaminophen; groups 6, 7, and 8 = 200 mg/kg, 100 mg/kg bw, and 50 mg/ kg bw of acetone extract, respectively, + 2 g/kg bw acetaminophen; and group 9 = 100 mg/kg silymarin + 2 g/kg bw acetaminophen. The phytochemical screening of the methanol and acetone leaves extracts showed the presence of flavonoids, alkaloids, saponins, anthocyanins, tannins, and triterpene, whereas, cardiac glycoside, steroids, and anthraquinone were absent in both extracts. Acetaminophen administration significantly elevated the levels of serum urea, creatinine, sodium, and potassium with a corresponding decrease in the levels of total protein, albumin, and calcium in the group 2 rats compared with that in the group 1 rats. Similarly, the levels of superoxide dismutase, catalase, glutathione peroxidase, glutathione, and glutathione S-transferase were significantly less in the acetaminophen-intoxicated group than that in the negative control group. However, pretreatment with either extracts, dose dependently prevented the acetaminophen-induced derangement of the aforementioned parameters. The extracts showed antioxidant activity similar to the reference drug (silymarin). Comparatively, the methanol extract gave higher in vivo antioxidant and nephroprotective effects than the acetone extract. The results suggest the extracts of cassava leaves have high nephroprotective potential and may be based on their phytoconstituents and antioxidant activity.

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Author(s):

Israel O. Okoro:[PubMed][Scholar]
Helen E. Kadiri:[PubMed][Scholar]
Eferhire Aganbi:[PubMed][Scholar]