Ethanol Extract of Jati Belanda (Guazuma ulmifolia L.) as Therapy for Chronic Kidney Disease in In Vitro Model

Sijani Prahastuti; Meilinah Hidayat; Stella Tinia Hasiana; Wahyu Widowati; Annisa Amalia; Rismawati Laila Qodariah; Rizal Rizal; Hanna Sari Widya Kusuma; Zakiyatul Khoiriyah

doi:10.4103/jrptps.JRPTPS_41_18

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https://doi.org/10.4103/jrptps.JRPTPS_41_18

Ethanol Extract of Jati Belanda (Guazuma ulmifolia L.) as Therapy for Chronic Kidney Disease in In Vitro Model

Author(s):

Sijani Prahastuti¹,

Meilinah Hidayat¹,

Stella Tinia Hasiana¹,

Wahyu Widowati^1,*,

Annisa Amalia²,

Rismawati Laila Qodariah²,

Rizal Rizal²,

Hanna Sari Widya Kusuma²,

Zakiyatul Khoiriyah²

1Faculty of Medicine, Maranatha Christian University, Jl Prof. Drg. Surya Sumantri 65, Bandung 40164, West Java, Indonesia

2Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl Babakan Jeruk II No 9, Bandung 40163, West Java, Indonesia

Journal of Reports in Pharmaceutical Sciences:Vol. 8, issue 2; 229-35

Published online:Dec 31, 2019

Article type:Research Article

How to Cite:Sijani PrahastutiMeilinah HidayatStella Tinia HasianaWahyu WidowatiAnnisa AmaliaRismawati Laila QodariahRizal RizalHanna Sari Widya KusumaZakiyatul Khoiriyahet al.Ethanol Extract of Jati Belanda (Guazuma ulmifolia L.) as Therapy for Chronic Kidney Disease in In Vitro Model.J Rep Pharm Sci.8(2):e147386.https://doi.org/10.4103/jrptps.JRPTPS_41_18.

Abstract

Background: People with diabetes mellitus in Indonesia are predicted to increase until 2035. High glucose in body (hyperglycemia) leads to increased fibronectin synthesis. Fibronectin that gets accumulated in glomerulus (mesangial cells), at the end, will lead to diabetic glomerulosclerosis. Jati belanda (Guazuma ulmifolia L.) leaf is well known as an Indonesian traditional medicine to have effects as antidiabetic by the presence of its secondary metabolites such as alkaloid, tannin, saponin, flavonoid, and terpenoid, which are very important in health recovery.
Objectives: To evaluate the activity of ethanol extract of jati belanda (EEJB) as a protection agent on induced-glucose mesangial cells of SV40 MES 13 cell line (glomerular mesangial kidney, Mus musculus).
Materials and Methods: EEJB (3.125 and 6.25 μg/mL) was extracted based on maceration method using ethanol (70%) as the solvent. Proliferation and viability were performed based on (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)- 2H-tetrazolium) (MTS) method. The level of transforming growth factor β1 (TGF-β1) and fibronectin in glucose-induced mesangial cells was assayed and determined using enzyme-linked immunosorbent assay kit. Reactive oxygen species (ROS) level was measured using flow cytometer.
Results: EEJB (3.125 and 6.25 μg/mL) increased cell proliferation and viability in glucose-induced mesangial cells and significantly reduced the level of TGF-β1, fibronectin, and ROS compared to that in positive control (glucose-induced cells).
Conclusion: Our study suggests that EEJB is able to reduce TGF-β1, fibronectin, and ROS levels in glucose-induced mesangial cells, which correlate to diabetic glomerulosclerosis condition and increase the mesangial cell proliferation and viability.

Keywords

Fibronectin

glomerulosclerosis

Guazuma ulmifolia

reactive oxygen species

transforming growth factor β

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Author(s):

Sijani Prahastuti:[PubMed][Scholar]
Meilinah Hidayat:[PubMed][Scholar]
Stella Tinia Hasiana:[PubMed][Scholar]
Wahyu Widowati:[PubMed][Scholar]
Annisa Amalia:[PubMed][Scholar]
Rismawati Laila Qodariah:[PubMed][Scholar]
Rizal Rizal:[PubMed][Scholar]
Hanna Sari Widya Kusuma:[PubMed][Scholar]
Zakiyatul Khoiriyah:[PubMed][Scholar]