Green Peas Protein Hydrolyzed by Bromelain in Simple Procedure to Improve Kidney Function in Cisplatin-induced Rats

Meilinah Hidayat; Sijani Prahastuti; Teresa Liliana Wargasetia; Kirana Nugraha; Andreanus Andaja Soemardji; Siti Farah Rahmawati; Nova Suliska; Khomaini Hasan

doi:10.4103/jrptps.jrptps_15_17

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https://doi.org/10.4103/jrptps.jrptps_15_17

Green Peas Protein Hydrolyzed by Bromelain in Simple Procedure to Improve Kidney Function in Cisplatin-induced Rats

Author(s):

Meilinah Hidayat^1,*,

Sijani Prahastuti²,

Teresa Liliana Wargasetia³,

Kirana Nugraha¹,

Andreanus Andaja Soemardji⁴,

Siti Farah Rahmawati⁴,

Nova Suliska⁴,

Khomaini Hasan⁵

1Nutrition Department, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia

2Biochemistry Department, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia

3Biology Department, Faculty of Medicine, Universitas Kristen Maranatha, Bandung, Indonesia

4Pharmacology Department, Pharmacy School, Instittut Teknologi Bandung, Bandung, Indonesia

5Biochemistry Department, Jenderal Achmad Yani University, Jawa Barat, Indonesia

Journal of Reports in Pharmaceutical Sciences:Vol. 8, issue 1; 68-77

Published online:Jun 30, 2019

Article type:Research Article

How to Cite:Meilinah HidayatSijani PrahastutiTeresa Liliana WargasetiaKirana NugrahaAndreanus Andaja SoemardjiSiti Farah RahmawatiNova SuliskaKhomaini Hasanet al.Green Peas Protein Hydrolyzed by Bromelain in Simple Procedure to Improve Kidney Function in Cisplatin-induced Rats.J Rep Pharm Sci.8(1):e147479.https://doi.org/10.4103/jrptps.jrptps_15_17.

Abstract

Context: Chronic kidney disease (CKD) can lead to terminal kidney failure. Previous study has shown that protein hydrolysate in yellow peas (Pisum sativum L.) can be used as a natural remedy for CKD.
Aims: To obtain hydrolysate protein that is most effective in improving kidney function of cisplatin (CP)‑induced Wistar rats, based on urea, creatinine, atrial natriuretic peptide (ANP), cyclooxygenase‑1 (COX‑1), and renin levels of CP‑induced nephrotoxicity Wistar rats.
Materials and Methods: Methods of Kjeldahl, Bradford, Kunitz, and sodium dodecyl sulfate‑polyacrylamide gel electrophoresis were used to determine the content of the eight types of protein hydrolysates. In in vivo experiment, the samples were administered to CP‑induced nephrotoxicity Wistar rats, with urea, creatinine, ANP, COX‑1, and renin as parameters.
Results: Total neutrase activity was 40.65 U/mg, and bromelain was 35.77 U/mg. Total specific activities of both enzymes were almost identical. Protein hydrolyzed using bromelain had small fractions (<14.4 kDa). On the 30th day of treatment, urea and creatinine levels of all groups of treatment were significantly different from CP control (P < 0.01). The lowest level was shown by the group which was treated with bromelain‑hydrolyzed green pea protein. Among ANP, COX‑1, and renin measurements, only the result of COX‑1 showed the promising result.
Conclusions: Green peas protein hydrolysate hydrolyzed by bromelain are suggested as the most effective in improving kidney function based on urea, creatinine, and COX-1 levels of CP-induced nephrotoxicity Wistar rats.

Keywords

Atrial natriuretic peptide

bromelain

cisplatin‑induced nephrotoxicity

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Author(s):

Meilinah Hidayat:[PubMed][Scholar]
Sijani Prahastuti:[PubMed][Scholar]
Teresa Liliana Wargasetia:[PubMed][Scholar]
Kirana Nugraha:[PubMed][Scholar]
Andreanus Andaja Soemardji:[PubMed][Scholar]
Siti Farah Rahmawati:[PubMed][Scholar]
Nova Suliska:[PubMed][Scholar]
Khomaini Hasan:[PubMed][Scholar]