β-Hematin Formation Assay for Screening of Potential Antimalarial Activity of The Extracts and Fractions of three Artemisia Species

Fariba Heshmati Afshar; Laleh Khodaie; Abbas Delazar

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β-Hematin Formation Assay for Screening of Potential Antimalarial Activity of The Extracts and Fractions of three Artemisia Species

Author(s):

Fariba Heshmati Afshar^{1, 2},

Laleh Khodaie^{1, 3,*},

Abbas Delazar²

1Medical Philosophy and History Research Center, Tabriz, Iran

2Departments of Pharmacognosy, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

3Departments of Phytopharmacy, Faculty of Traditional Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 7, issue 2; 214-220

Published online:May 22, 2018

Article type:Research Article

Received:Mar 09, 2018

Accepted:May 15, 2018

How to Cite:Fariba Heshmati AfsharLaleh KhodaieAbbas Delazarβ-Hematin Formation Assay for Screening of Potential Antimalarial Activity of The Extracts and Fractions of three Artemisia Species.J Rep Pharm Sci.7(2):e147589.

Abstract

In this study, In-vitro β-hematin formation assay, a spectrophotometric assessment is used to screen potential antimalarial effects of Artemisia extracts as well as their fractions. The n-hexane (n-hex), dichloromethane (DCM) and methanolic (MeOH) extracts obtained from aerial parts of A. splendens and A. austriaca by soxhlet apperatus, also DCM fractions, which were acquired by VLC method using n-hex, ethyl acetate (EtOAC) and MeOH, of A. spicigera, A. splendens and A. austriaca were examined by the mentioned method. N-hex and MeOH extracts of the herbs indicated weak anti-malarial activities, whereas, the DCM extracts of A. spicigera (IC50= 0.99±0.01mg/ml, which had been reported in previous manuscript), A. splendens (IC50= 1.93±0.09 mg/ml) and A. austriaca (IC50= 1.15±0.04mg/ml) showed potent antimalarial effects. Among the all examined DCM fractions with different polarities, DCM F4΄΄ (60% EtOAC/n-hex, the DCM fraction of A. austriaca) with IC50 value of 1.02 ± 0.03 mg/ml, DCM F5 (80% EtOAC/n-Hex, the DCM fraction of A. spicigera) with IC50 value of 1.21± 0.06 mg/ml, DCM F6 (100% EtOAC, the DCM fraction of A. austriaca) and DCM F7΄΄ (100% MeOH, the DCM fraction of A. austriaca) with IC50 values of 1.44 ± 0.04 mg/ml for both, as well as DCM F7΄ (100% MeOH, the DCM fraction of A. splendense) with IC50 value of 1.58 ± 0.03 mg/ml indicated higher activities than the other DCM fractions. The mentioned fractions could be useful to bioassay-guided isolation of bioactive plant constituents.

Keywords

Artemisia

Asteraceae

Dichloromethane Extract

In-Vitro Malaria

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