Two Phase Solvent Extraction Method for Analysis of Methadon in Immature Human Milk, A Breast Feeding Recommendation in Early Postpartum Period

Nazanin Jalilian; Farhad Ahmadi; Elham Farhadi

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Two Phase Solvent Extraction Method for Analysis of Methadon in Immature Human Milk, A Breast Feeding Recommendation in Early Postpartum Period

Author(s):

Nazanin Jalilian¹,

Farhad Ahmadi^{2, 3,*},

Elham Farhadi¹

1Department of Obstetrics and Gynecology, Maternity Research Center, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Department of Medicinal Chemistry, School of Pharmacy-International Campus, University of Medical Sciences, Tehran, Iran

3Nano Drug Delivery Research Center, Kermanshah University of Medical Science, Kermanshah, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 7, issue 2; 9-19

Published online:Oct 11, 2017

Article type:Research Article

Received:Jul 25, 2017

Accepted:Sep 02, 2017

How to Cite:Nazanin JalilianFarhad AhmadiElham FarhadiTwo Phase Solvent Extraction Method for Analysis of Methadon in Immature Human Milk, A Breast Feeding Recommendation in Early Postpartum Period.J Rep Pharm Sci.7(2):e147595.

Abstract

In this work we introduced a new two phase freezing (TPF) method coupled with gas chromatography for the extraction, clean up and determination of methadone (MT) in human milk samples. TPF procedure was optimized for extraction of MT from immature milk sample. The extraction of MT was performed from 1.0 ml of milk that contain 0.2 ml of Briton Robinson buffer (pH=2.5) and 0.3 ml of acetonitrile. For separation of acetonitrile from aqueous solution, the solution was placed in refrigerator at −40 °C. The MT was analyzed by gas chromatography. The results demonstrated that the amount of MT that transferred to milk is significantly different from other published reports. The immature milks of six women who were used MT (dose of 90 mg/day) in duration of 1, 2, 3, and 5 h after consumption were analyzed. Our data demonstrated that, before one hour and also 5 h after MT consumption the breastfeeding is safe and between 2-4 h after consumption dose not safe neither.

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