Effects of Rhus Coriaria L. (Sumac) Extract on Hepatitis B Virus Replication and Hbs Ag Secretion

Amin Gharabolagh Farzanegan; Farzaneh Sabahi; Mohsen Karimi; Mohammad Kamalinejad; Hessam Mirshahabi; Seyed Dawood Mousavi Nasab; Nayeb Ali Ahmadi

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Effects of Rhus Coriaria L. (Sumac) Extract on Hepatitis B Virus Replication and Hbs Ag Secretion

Author(s):

Amin Gharabolagh Farzanegan¹,

Farzaneh Sabahi^1,*,

Mohsen Karimi²,

Mohammad Kamalinejad³,

Hessam Mirshahabi⁴,

Seyed Dawood Mousavi Nasab⁵,

Nayeb Ali Ahmadi⁶

1Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

2Medical Genetics, Department of Medical Neuroscience, Center for Molecular Medicine, Karolinska Institute, Stockholm, Sweden

3Faculty of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4Department of Microbiology and Immunology, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran

5Departments of Research and Development, Production and Research Complex, Pasteur Institute of Iran

6Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 7, issue 1; 100-107

Published online:Jan 07, 2018

Article type:Research Article

Received:Oct 05, 2017

Accepted:Dec 25, 2017

How to Cite:Amin Gharabolagh FarzaneganFarzaneh SabahiMohsen KarimiMohammad KamalinejadHessam MirshahabiSeyed Dawood Mousavi NasabNayeb Ali Ahmadiet al.Effects of Rhus Coriaria L. (Sumac) Extract on Hepatitis B Virus Replication and Hbs Ag Secretion.J Rep Pharm Sci.7(1):e147601.

Abstract

The current therapies against hepatitis B virus (HBV) infection are only effective for a number of patients. Mutant viruses that are resistant to treatment and get created due to the errors in the reverse transcriptase mechanism cause for prolonged treatments. In this regard, recent studies have been focused on new therapeutic approaches especially herbal medications to overcome the problem in resistant patients. The aim of this study was to evaluate the inhibitory effect of Rhus coriaria L. (Sumac) aqueous extract on hepatitis B virus replication and HBsAg secretion in HuH-7 cell line after transfection by HBV. Huh-7 cell line was transfected by a plasmid (pCH-9/3091) containing HBV genome using Lipofectamine 2000. The aqueous extract of Rhus coriaria L.was prepared and its cytotoxicity on transfected cells was assessed by MTT assay. The inhibitory effect of the aqueous extract of Rhus coriaria L.on release of HBsAg as a consequence of HBV replication was measured by ELISA. Our results showed a significant lower concentration of HBsAg after exposure to Rhus coriaria L.extract compared to the untreated control group and positive control group treated with Lamivudine of HBV transfected Huh-7 cell line. This study represents the inhibitory effect of sumac aqueous extract on multiplication of HBV and its antigen secretion.

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