Hepatoprotective Effects of Combination Hydroalcoholic Extracts of Nigella Sativa and Curcuma Longa on Adriamycin- Induced Oxidative Stress in Rat

authors:

avatar Reza Mohebbati 1 , avatar Mohammad Reza Khazdair 2 , 3 , avatar Sareh Karimi 1 , avatar Abbasali Abbasnezhad 4 , *

Department of Physiology, School of Medicine, Medical University of Mashhad, Iran
Pharmaceutical Research Center and Department of Physiology School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran
Department of Basic Sciences, Faculty of Medicine, Gonabad University of Medical Sciences, Gonabad, Iran
Journal of Reports in Pharmaceutical Sciences: Vol.6, issue 2; 105-114
published online: June 10, 2017
article type: Research Article
received: April 18, 2016
revised: June 22, 2016
accepted: August 17, 2016

how to cite: Mohebbati R, Khazdair M R, Karimi S, Abbasnezhad A. Hepatoprotective Effects of Combination Hydroalcoholic Extracts of Nigella Sativa and Curcuma Longa on Adriamycin- Induced Oxidative Stress in Rat. J Rep Pharm Sci. 2017;6(2):e147608. 

Abstract

The ample of studies show that Adriamycin caused hepatotoxicity in rat. The present study investigated the effects of Nigella Sativa (N. Sativa) and combination with Curcuma longa (C. longa) extract on Adriamycin-induced hepatotoxicity in rat.
Rats were divided into eight experimental groups: Control (CO), Adriamycin (ADR), Vitamin C (Vit C), Adriamycin with Vitamin C (ADR+VitC), N. Sativa with and without Adriamycin (NS +ADR, NS -ADR), Combination extract of C. longa and N. Sativa with and without Adriamycin (NS+CL+ADR, NS+CL-ADR). Malondialdehyde (MDA) and thiol levels and also the activities of catalase (CAT) in liver tissue were evaluated.
MDA level in the liver tissue in ADR was increased compared to CO group but in NS+ADR group, ADR+VitC and NS –ADR groups decreased compared to ADR group. Thiol levels in ADR and ADR+VitC groups were decreased compared to CO group. Thiol levels in treatment groups were increased compared to ADR group. The activities of CAT in liver tissue of ADR group were lower than CO group, and increased in treatment groups comparison with ADR group.
The results showed that chronic administration of N. sativa hydroalcoholic extract in Adriamycin-induced hepatotoxicity rats could decrease the oxidative stress injuries in liver tissue.