Evaluation of Cytotoxic Effects of a DinuclearPalladacycle Derivative, Biphosphinic Complex, on Cisplatin-Resistant HT- 29 Cells

Sepideh Shahsavari-Alavijeh; Hojjat Sadeghi-Aliabadi; Ali Jahanian-Najafabadi; mina mirian

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Abstract
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Evaluation of Cytotoxic Effects of a DinuclearPalladacycle Derivative, Biphosphinic Complex, on Cisplatin-Resistant HT- 29 Cells

Author(s):

Sepideh Shahsavari-Alavijeh¹,

Hojjat Sadeghi-Aliabadi^1,*,

Ali Jahanian-Najafabadi^{2, 3},

mina mirian²

1Department of Pharmaceutical Chemistry, Isfahan University of Medical Sciences, Isfahan, Iran

2Department of Pharmaceutical Biotechnology, Isfahan University of Medical Sciences, Isfahan, Iran

3Isfahan Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 6, issue 2; 115-122

Published online:Jun 10, 2017

Article type:Research Article

Received:Mar 09, 2017

Accepted:May 27, 2017

How to Cite:Sepideh Shahsavari-AlavijehHojjat Sadeghi-AliabadiAli Jahanian-Najafabadimina mirianEvaluation of Cytotoxic Effects of a DinuclearPalladacycle Derivative, Biphosphinic Complex, on Cisplatin-Resistant HT- 29 Cells.J Rep Pharm Sci.6(2):e147609.

Abstract

Previous studies showed that palladacycle complexes with three phenyl phosphine ligands and piperidine or biphosphinic based palladacycle complexes were cytotoxic on K562, HT29, and Hela cell lines. In the present study, in order to evaluate the efficacy of the compound on cisplatin resistant cells, first we made HT-29 cells resistant to cisplatin, and then evaluated the potential cytotoxicity of the mentioned plladacylce complex on them. In addition, it was evaluated whether cytotoxic effect of the compound is mediated via apoptosis or necrosis death mechanisms. In this regard, Annexin V/PI by staining followed by flow cytometric analysis was performed. Results showed that palladacycle complexes were 45 times more cytotoxic than cisplatin (P<0.05) on the resistant HT-29 cells. Flow cytometry results also revealed that apoptosis induction was the major cell death mechanism of these compounds. Therefore, it could be concluded that these compounds might be effectively cytotoxic for cisplatin resistant cells. However, further in vitro and in vivo preclinical studies on evaluation of specific and non-specific cytotoxic characteristics of these complexes are necessary.

Keywords

Apoptosis

Cisplatin resistant cells

HT-29 cells

MTT assay

Palladacyclic complex

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