Study of Callus Induction and Cell Culture to Secondary Metabolite Production in Hyssopus officinalis L.

Gulsum Pakseresht; Danial Kahrizi; Mohsen Mansouri; Tayebeh Ghorbani; Nastaran Kazemi

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Abstract
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Study of Callus Induction and Cell Culture to Secondary Metabolite Production in Hyssopus officinalis L.

Author(s):

Gulsum Pakseresht¹,

Danial Kahrizi^{2, 3,*},

Mohsen Mansouri^{3, 4},

Tayebeh Ghorbani^{3, 4},

Nastaran Kazemi^{1, 3}

1Department of Medicinal Plants, Institute of Higher Education, Jahad-e-Daneshgahi, Kermanshah Unit, Kermanshah, Iran

2Agronomy and Plant Breeding Department, Razi University, Kermanshah, Iran

3Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

4Zagros Bioidea Co. Razi University Incubator, Kermanshah, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 5, issue 2; 104-111

Published online:Sep 25, 2016

Article type:Research Article

Received:Jun 27, 2016

Accepted:Sep 05, 2016

How to Cite:Gulsum PaksereshtDanial KahriziMohsen MansouriTayebeh GhorbaniNastaran KazemiStudy of Callus Induction and Cell Culture to Secondary Metabolite Production in Hyssopus officinalis L..J Rep Pharm Sci.5(2):e147650.

Abstract

The Hyssopus officinalis L. is an important medicinal plant. Antimicrobial and antifungal activities of the essential oil of hyssop have been reported. In this article the effects of explants types, plants growth regulators and elicitors on callus induction and cell culture conditions were studied. In this experiment it was found that there were non- significant differences among hypocotyl and leaf H. officinalis explants for callus induction and callus growth rate. There were significant differences among levels of growth regulators and interaction effect between growth regulators and expellant types for callus induction and callus growth rate. There were significant differences among plant growth regulators levels for callus induction and callus growth rate. Results showed that medium supplemented by N2B1 and N0.5B1 showed the highest callus induction and callus growth rate respectively. In this paper it was demonstrated the important role of plant growth regulators and explant types on callus induction in H. Officinalis as a medicinal plants. Also the most important secondary metabolites in H. officinalis were increased in cell culture presence of salicylic acid, citric acid and yeast extract elicitors.

Keywords

Hyssopusofficinalis L.

Plant growth regulator

Elicitor

Yeast

Secondary metabolite

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