The Effect of Cis and Trans Vaccenic Acids on Expression of ICAM-1 And VCAM-1 in Human Microvascular Endothelial Cells (HMEC)

Abbas Abbasi; Ali Mostafaei; Gholamreza Bahrami; Kamran Mansouri; Sajad Sisakhtnejad

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The Effect of Cis and Trans Vaccenic Acids on Expression of ICAM-1 And VCAM-1 in Human Microvascular Endothelial Cells (HMEC)

Author(s):

Abbas Abbasi¹,

Ali Mostafaei^1,*,

Gholamreza Bahrami¹,

Kamran Mansouri^{1, 2,**},

Sajad Sisakhtnejad¹

1Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

2Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Tehran, Iran

Corresponding Authors:

Journal of Reports in Pharmaceutical Sciences:Vol. 4, issue 1; 65-74

Published online:May 23, 2015

Article type:Research Article

Received:Mar 05, 2015

Accepted:May 20, 2015

How to Cite:Abbas AbbasiAli MostafaeiGholamreza BahramiKamran MansouriSajad SisakhtnejadThe Effect of Cis and Trans Vaccenic Acids on Expression of ICAM-1 And VCAM-1 in Human Microvascular Endothelial Cells (HMEC).J Rep Pharm Sci.4(1):e147690.

Abstract

Trans-fatty acids (TFA) are known as a risk factor for coronary artery diseases. Trans-11 vaccenic acid [VA; 18:1(n-9)], known as the major trans fatty acid in milk fat, is a positional and geometric isomer of oleic acid and a precursor of conjugated linoleic acid (CLA) in human . This study was carried out in order to investigate the effect of Cis and Trans Vaccenic Acids on expression of intra cellular adhesion molecul-1 (ICAM-1) and vascular cellular adhesion molecul-1 (VCAM-1) in human Microvascular endothelial cells (HMECs). To induce adhesion molecules expression, TNF-α or LPS were added to HMECs in a cell culture being treated with Cis and Trans vaccenic acid. In the next step, to evaluate ICAM-1 and VCAM-1 expression before and after the treatment, Western blot technique was used. The analyzed results indicated that Cis and Trans vaccenic acids are able to suppress VCAM-1 and ICAM-1 expression on HMEC near to the basal level. Also it has been found that cis vaccenic acid isomer could suppress both VCAM-1 and ICAM-1 expression as well as trans isomer. Together all and considering the role of mentioned adhesion molecules in atherosclerosis; it seems that Cis and trans vaccenic acids could be a possible prophylaxis agents in populations with high risk of atherosclerosis.

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