Detection of corticosteroid compounds and phosphodiester- ase inhibitors (PDH-5) as counterfeit in herbal products available in Iranian market by HPLC method

Ronak Jalili; Shahram Miraghaei; Bahareh Mohamadi; Atefeh Babaei; Gholamreza Bahrami

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Detection of corticosteroid compounds and phosphodiester- ase inhibitors (PDH-5) as counterfeit in herbal products available in Iranian market by HPLC method

Author(s):

Ronak Jalili^1,*,

Shahram Miraghaei¹,

Bahareh Mohamadi²,

Atefeh Babaei¹,

Gholamreza Bahrami^{1, 2}

1Medical Biology Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

2School of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran

Journal of Reports in Pharmaceutical Sciences:Vol. 4, issue 1; 75-81

Published online:May 30, 2015

Article type:Research Article

Received:Feb 05, 2015

Accepted:May 25, 2015

How to Cite:Ronak JaliliShahram MiraghaeiBahareh MohamadiAtefeh BabaeiGholamreza BahramiDetection of corticosteroid compounds and phosphodiester- ase inhibitors (PDH-5) as counterfeit in herbal products available in Iranian market by HPLC method.J Rep Pharm Sci.4(1):e147691.

Abstract

Weight-gain and potency enhancing drugs are the most popular herbal products in Iran which are easily available via the internet and through illegal markets. The content of some of these popularly purchased products were investigated for the presence of illegal substance like phosphodiesterase inhibitors (PDH-5) and corticosteroids by HPLC method. For this study, ten samples of both kind of herbal medicine were obtained from Iranian markets, then chromatographic analysis of corticosteroids was achieved isocratically on a C18 column (C18, 5μm , 150 mm x 4.6 mm) by utilizing a mobile phase of methanol/water (55:45, v/v, pH 7.0) at a flow rate of 1.5 mL/min with UV detection at 250 nm, while analysis of phosphodiesterase inhibitors followed by a C18 column (C18, 5μm, 150 mm x 4.6 mm) using a mobile phase of methanol/water (65:35 v/v, diethyl amine(100μl/l, pH 3.5) at a flow rate of 1.5 mL/min. UV detector operated at 290 nm. After quantitative analysis, different contents of sildenafil and tadalafil in 3 cases of enhancing herbal remedies and dexamethazone in 3cases of weight gaining herbal medicine have been identified.

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